Effect of Korean Red Ginseng on the Stability of the Tight Junction of Intestinal Epithelial Cells

홍삼에 의한 Caco-2 단세포층 간극의 안정화

  • Received : 2009.09.04
  • Accepted : 2010.01.21
  • Published : 2010.06.30

Abstract

Bioactive components involved in the tight junction stabilization of intestinal epithelial cells from Korean red ginseng were studied by analyzing transepithelial electrical resistance (TEER) values of the Caco-2 cell monolayer between the apical and basolateral sides for 96 hr. The treatment with less than $20\;{\mu}g/mL$ of the Korean red ginseng extract to the apical side of Caco-2 cell monolayer gave higher TEER values than the control. However, the treatment with more than $130\;{\mu}g/mL$ of the Korean red ginseng extract drastically decreased the TEER values, and these effects were not due to its cytotoxicity. When fractions of low molecular weight compounds, polysaccharides, proteins, saponins, and polyphenols derived from Korean ginseng were applied to the apical side of the Caco-2 cell monolayer, polyphenols showed high tight junction stabilizing activity and saponins showed low activity, but the others showed no significant activity. These results suggest that Korean red ginseng might be useful for the prevention of food allergy by stabilizing the tight junction of intestinal epithelial cells leading to hindering absorption of food allergens.

Keywords

Korean red ginseng;Caco-2 cell;tight junction;transepithelial electrical resistance (TEER)

References

  1. Fasano A, Shea-Donohue T. Mechanisms of Disease: The role of intestinal barrier function in the pathogenesis of gastrointestinal autoimmune diseases. Nat. Clin. Pract. Gastr. 2: 416-422 (2005)
  2. Lewis SA, Berg JR, Kleine TJ. Modulation of epithelial permeability by extracellular macromolecules. Physiol. Rev. 75: 561-589 (1995)
  3. Majamaa H, Isolauri E. Evaluation of the gut mucosal barrier: Evidence for increased antigen transfer in children with atopic eczema. J. Allergy Clin. Immun. 97: 985-990 (1996) https://doi.org/10.1016/S0091-6749(96)80074-1
  4. Perdue MH. Mucosal immunity and inflammation. III. The mucosal antigen barrier: Cross talk with mucosal cytokines. Am. J. Physiol. 277: G1-G5 (1999)
  5. Gu L, Li N, Li Q, Zhang Q, Wang C, Zhu W, Li J. The effect of berberine in vitro on tight junctins in human Caco-2 intestinal epithelial cells. Fitoterapia 80: 241-248 (2009) https://doi.org/10.1016/j.fitote.2009.02.005
  6. Araki Y, Katoh Y, Ogawa A, Bamba S, Andoh A, Koyama S, Fujiyama Y, Bamba T. Bile acid modulates transepithelial permeability via the generation of reactive oxygen species in the Caco-2 cell line. Free Radical Bio. Med. 39: 769-780 (2005) https://doi.org/10.1016/j.freeradbiomed.2005.04.026
  7. DeMeo MT, Mutlu EA, Keshavarzian A, Tobin MC. Intestinal permeation and gastrointestinal disease. J. Clin. Gastroenterol. 34: 385-396 (2002) https://doi.org/10.1097/00004836-200204000-00003
  8. Shoko T, Michiko W. An active compound against allergen absorption in hypoallergenic wheat flour produced by enzymatic modification. Biosci. Biotech. Bioch. 66: 1930-1935 (2002) https://doi.org/10.1271/bbb.66.1930
  9. Kenarova B, Neychev H, Hadjiivanova C, Petkov VD. Immunomodulating activity of ginsenoside Rg1 from Panax ginseng. Jpn. J. Pharmacol. 54: 447-454 (1990) https://doi.org/10.1254/jjp.54.447
  10. Kang KS, Yokozawa T, Kim HY, Park JH. Study on the nitric oxide scavenging effects of ginseng and its compounds. J. Agr. Food Chem. 54: 2558-2562 (2006) https://doi.org/10.1021/jf0529520
  11. Gao H, Wang F, Lien EJ, Trousdale MD. Immunostimulating polysaccharides from Panax notoginseng. Pharm. Res. 13: 1196-1200 (1996) https://doi.org/10.1023/A:1016060119425
  12. Park S, Yeo M, Jin JH, Lee KM, Jung JY, Choue R, Cho SW, Hahm KB. Rescue of Helicobacter pylori-induced cytotoxicity by red ginseng. Digest. Dis. Sci. 50: 1218-1227 (2005) https://doi.org/10.1007/s10620-005-2763-x
  13. Park S, Yeo M, Jin JH, Lee KM, Kim SS, Chio SY, Hahm KB. Inhibitory activities and attenuated expressions of 5-LOX with red ginseng in Helicobacter pylori-infected gastric epithelial cells. Digest. Dis. Sci. 52: 973-982 (2007) https://doi.org/10.1007/s10620-006-9440-6
  14. Lee JY, Shin JW, Chun KS. Antitumor promotional effects of a novel intestinal bacterial metabolite (IH-901) derived from the protopanaxadiol-type ginsenosides in mouse skin. Carcinogenesis 26: 359-367 (2005)
  15. Bae EA, Hyun YJ, Choo MK, Oh JK. Protective effect of fermented red ginseng on a transient focal ischemic rats. Arch. Pharm. Res. 27: 2236-2240 (2004)
  16. Choo MK, Park EK, Han MJ, Kim DH. Antiallergic activity of ginseng and its ginsenosides. Planta Med. 69: 518-522 (2003) https://doi.org/10.1055/s-2003-40653
  17. Choi CS, Kim KY, Hong HD, Kim YC. Phenolic acid composition and antioxidative activity of white ginseng (Panax ginseng, C.A. Meyer). J. Ginseng Res. 30: 22-30 (2006) https://doi.org/10.5142/JGR.2006.30.1.022
  18. Hashimoto K, Nakayama T, Shimizu M. Effects of beta-lactoglobulin on the tight-junctional stability of Caco-2-SF monolayer. Biosci. Biotech. Bioch. 62: 1819-1821 (1998) https://doi.org/10.1271/bbb.62.1819
  19. Park EK, Choo MK, Kim EJ, Han MJ, Kim DH. Antiallergic activity of ginsenoside Rh2. Biol. Pharm. Bull. 26: 1581-1584 (2003) https://doi.org/10.1248/bpb.26.1581
  20. Park EK, Choo MK, Han MJ, Kim DH. Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities. Int. Arch. Allergy Imm. 133: 113-120 (2004) https://doi.org/10.1159/000076383
  21. Choi HJ, Han HS, Choi C. Antioxidative, phospholipase A2 inhibiting, and anticancer effect of polyphenol rich fractions from Panax ginseng C.A. Meyer. J. Korean Soc. Agric. Chem. Biotechnol. 46: 251-256 (2003)