Immunomodulating Activity of Laminaria japonica Polysaccharides

참다시마 다당체의 면역 증강 활성

  • Ryu, Deok-Seon (Department of Biomedical Laboratory Science, Inje University) ;
  • Oh, Seung-Min (Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Ki-Hoon (Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Soo-Hwan (Department of Biomedical Laboratory Science, Inje University) ;
  • Choi, Hyun-Ju (Department of Biomedical Laboratory Science, Inje University) ;
  • Lee, Dong-Seok (Department of Biomedical Laboratory Science, Inje University)
  • 류덕선 (인제대학교 의생명공학대학 임상병리학과) ;
  • 오승민 (인제대학교 의생명공학대학 임상병리학과) ;
  • 김기훈 (인제대학교 의생명공학대학 임상병리학과) ;
  • 김수환 (인제대학교 의생명공학대학 임상병리학과) ;
  • 최현주 (인제대학교 의생명공학대학 임상병리학과) ;
  • 이동석 (인제대학교 의생명공학대학 임상병리학과)
  • Received : 2009.12.30
  • Accepted : 2010.03.02
  • Published : 2010.06.30

Abstract

Laminaria japonica polysaccharides (LP) were prepared from L. japonica through hot water extraction, ultrafiltration and gel chromatography. In this study, we investigated the immunomodulating activity of LP (0.25-1 mg/mL) on the mitogen/alloantigen reactive proliferation and killing activity of the Balb/c mouse splenocytes. The LP directly induced the proliferation of splenocytes that was stimulated with mitogen or alloantigen in a dose-dependent manner. The killing activity of cytotoxic T lymphocytes (CTLs) and lymphokine activated killer cells (LAKs) were enhanced significantly in the LP treated cells. Also, the treatment of splenocytes with LP increased production of interleukin-2 (IL-2). These results suggest that polysaccharides from L. japonica show a substantial immunomodulating activity in mouse immune cells.

Keywords

Laminaria japonica;polysaccharides;immunomodulating activity

Acknowledgement

Supported by : 한국학술진흥재단

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