Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea

국내 경북지역 소에서 분리된 브루셀라 분리주의 생물학적 특성

  • Kim, Jeong-Hwa (Northern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Lim, Jeong Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dong Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Jin Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dae Geun (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Sang Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Chang, Hong Hee (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hu Jang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
  • 김정화 (경상북도 가축위생시험소 북부지소) ;
  • 임정주 (경상대학교 수의과대학) ;
  • 김동혁 (경상대학교 수의과대학) ;
  • 이진주 (경상대학교 수의과대학) ;
  • 김대근 (경상대학교 수의과대학) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 김상훈 (충남대학교 수의과대학) ;
  • 장홍희 (경상대학교 농업생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학) ;
  • 민원기 (경상대학교 수의과대학) ;
  • 김석 (경상대학교 수의과대학)
  • Accepted : 2010.02.25
  • Published : 2010.06.30

Abstract

Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

Acknowledgement

Grant : 총체적 단백분석을 통한 국내분리 Brucella abortus 균의 항원성 규명과 고민감도 혈청학적 진단기법 개발

Supported by : 한국연구재단

References

  1. 김우택, 이완수, 김공식. 제주도내 축우 부루셀라병 발생상황 조사. 한국가축위생학회지 1991, 14, 104-109.
  2. 김종만, 정석찬, 박정문. 부루셀라 양성우에서 분리한 균의 성상과 혈청학적 진단법 비교. 농사시험논문집 1988, 30, 1-6.
  3. 농림수산식품부. 결핵병 및 브루셀라병 방역 실시 요령. 농림수산식품부 고시 제2009-147호. 농림수산식품부, 과천, 2009.
  4. 우종태, 서익수. 홀스타인 유우로부터 Brucella abortus 의 분리와 분리균의 성상에 관한 연구. 서울대학교 수의대 논문집 1986, 11, 103-118.
  5. 정종식, 조용준, 박청규. 경북지방 젖소로부터 Brucella abortus의 분리 및 균형별. 대한수의학회지 1988, 28, 339-343.
  6. 심항섭, 고태오, 유성종, 우종태, 박병옥, 김성렬, 박유순. 경기도에서 발생하는 유우부루셀라병에 관한 연구. I. 감염우의 역학조사 및 분리균의 특성에 관하여. 한국가축위생학회지 1996, 19, 189-198.
  7. Alton GG, Jones LM, Angus RD, Berger JM. Techniques for the Brucellosis Laboratory. pp. 1-156, Institut National de la Recherche Agronomique, Paris, 1988.
  8. Alton GG, Jones LM, Pietz DE. Laboratory Techniques in Brucellosis. 2nd ed. pp. 1-163, World Health Organization, Geneva, 1975.
  9. Alton GG, Maw J, Rogerson BA, McPherson GG. The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests. Aust Vet J 1975, 51, 57-63. https://doi.org/10.1111/j.1751-0813.1975.tb09404.x
  10. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 1992, 95, 271-275.
  11. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994, 32, 2660-2666.
  12. Bricker BJ, Halling SM. Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J Clin Microbiol 1995, 33, 1640-1642.
  13. Cloeckaert A, Verger JM, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology 1995, 141, 2111-2121. https://doi.org/10.1099/13500872-141-9-2111
  14. Cloeckaert A, Grayon M, Grepinet O, Boumedine KS. Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests. Microbes Infect 2003, 5, 593-602. https://doi.org/10.1016/S1286-4579(03)00091-1
  15. Corbel MJ, Gill KPW, Redwood DW. Diagnostic Procedures for Non-Smooth Brucella Strains. pp. 74-92, Central Veterinary Laboratory, Weybridge, 1979.
  16. Corbel MJ, Gill KPW, Thomas EL. Methods for the Identification of Brucella. pp. 4-35, Central Veterinary Laboratory, Weybridge, 1978.
  17. Fekete A, Bantle JA, Halling SM, Sanborn MR. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J Appl Bacteriol 1990, 69, 216-227. https://doi.org/10.1111/j.1365-2672.1990.tb01512.x
  18. Finegold SM, Martin WJ, Scott EG. Bailey and Scott's Diagnostic Microbiology. 5th ed. pp. 146-187, C. V. Mosby, St. Louis, 1978.
  19. Garcia-Rodriguez JA, Garcia Sanchez JE, Trujillano I, Garcia Sanchez E, Garcia Garcia MI, Fresnadillo MJ. Susceptibilities of Brucella melitensis isolates to clinafloxacin and four other new fluoroquinolones. Antimicrob Agents Chemother 1995, 39, 1194-1195. https://doi.org/10.1128/AAC.39.5.1194
  20. Herman L, De Ridder H. Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol 1992, 58, 2099-2101.
  21. International Office of Epizootics. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Mammals, Birds and Bees. 6th ed. pp. 624-659, OIE, Paris, 2008.
  22. Kim S, Watarai M, Suzuki H, Makino S, Kodama T, Shirahata T. Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus. Microb Pathog 2004, 37, 11-19. https://doi.org/10.1016/j.micpath.2004.04.002
  23. Leal-klevezas DS, Martinez-vazquez IO, Lopezmerino A, Martinez-Soriano JP. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol 1995, 33, 3087-3090.
  24. Lopez-Goni I, Moriyon I. Brucella: Molecular and Cellular Biology. pp. 1-428, Horizon Bioscience, Norfolk, 2004.
  25. Lord VR, Rolo MR, Cherwonogrodzky JW. Evaluation of humoral immunity to Brucella sp in cattle by use of an agar-gel immunodiffusion test containing a polysaccharide antigen. Am J Vet Res 1989, 50, 1813-1816.
  26. Morata P, Queipo-ortuno MI, De Dios Colmenero J. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol 1998, 36, 2443-2446.
  27. Nielsen K, Cherwonogrodzky JW, Duncan JR, Bundle DR. Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19. Am J Vet Res 1989, 50, 5-9.
  28. Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine. 10th ed. pp. 963-984, Saunders, Philadelphia, 2007.
  29. Romero C, Gamazo C, Pardo M, Lopez-Goni I. Specific detection of brucella DNA by PCR. J Clin Microbiol 1995, 33, 615-617.