- Volume 50 Issue 2
Comparative study of Clostridium perfringens, Salmonella spp. and E. coli focused on characteristics of E. coli O157 isolated from pigs of HACCP- and non-HACCP-accredited swine farms in Korea
- Keum, Hyun Ok (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
- Kim, Hye Kwon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
- Rho, Se Mi (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
- Moon, Hyoung Joon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
- Park, Seong Jun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
- Park, Bong Kyun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
- Accepted : 2010.03.05
- Published : 2010.06.30
To determine the prevalence of Escherichia (E.) coli O157 : H7 from pigs after the Hazard Analysis and Critical Control Point (HACCP) system has been applied to Korean swine farm since 2006, 291 fecal samples were tested between May and December in 2008. Four E. coli O157:non-H7 (1.4%) were isolated from 4 different non-HACCP-accredited farms and they didn't have virulent genes which can cause illness for human. Also, Clostridium (C.) perfringens, Salmonella spp. and E. coli enterotoxins were tested using multiplex PCR. The positive rate for these pathogens of non-HACCP-accredited farms was higher than that of HACCP-accredited farms, and especially in case of C. perfringens, E. coli enterotoxins LT and STa, it was statistically significant (p < 0.05). Thus, the early implementation of the HACCP program is expected to greatly contribute to the safety of livestock products as well as food hygiene.
Supported by : Rural Development Administration
- Botteldoorn N, Heyndrickx M, Rijpens N, Herman L. Detection and characterization of verotoxigenic Escherichia coli by a VTEC/EHEC multiplex PCR in porcine faeces and pig carcass swabs. Res Microbiol 2003, 154, 97-104. https://doi.org/10.1016/S0923-2508(03)00028-7
- Chapman PA, Siddons CA, Cerdan Malo AT, Harkin MA. A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol Infect 1997, 119, 245-250. https://doi.org/10.1017/S0950268897007826
- Feder I, Wallace FM, Gray JT, Fratamico P, Fedorka-Cray PJ, Pearce RA, Call JE, Perrine R, Luchansky JB. Isolation of Escherichia coli O157 : H7 from intact colon fecal samples of swine. Emerg Infect Dis 2003, 9, 380-383. https://doi.org/10.3201/eid0903.020350
- Gannon VP, D'Souza S, Graham T, King RK, Rahn K, Read S. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J Clin Microbiol 1997, 35, 656-662.
- Heuvelink AE, Zwartkruis-Nahuis JT, van den Biggelaar FL, van Leeuwen WJ, de Boer E. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from slaughter pigs and poultry. Int J Food Microbiol 1999, 52, 67-75. https://doi.org/10.1016/S0168-1605(99)00119-1
- Hulebak KL, Schlosser W. Hazard analysis and critical control point (HACCP) history and conceptual overview. Risk Anal 2002, 22, 547-552. https://doi.org/10.1111/0272-4332.00038
- Jo MY, Kim JH, Lim JH, Kang MY, Koh HB, Park YH, Yoon DY, Chae JS, Eo SK, Lee JH. Prevalence and characteristics of Escherichia coli O157 from major food animals in Korea. Int J Food Microbiol 2004, 95, 41-49. https://doi.org/10.1016/j.ijfoodmicro.2004.01.016
- Kim JY, Kim SH, Kwon NH, Bae WK, Lim JY, Koo HC, Kim JM, Noh KM, Jung WK, Park KT, Park YH. Isolation and identification of Escherichia coli O157 : H7 using different detection methods and molecular determination by multiplex PCR and RAPD. J Vet Sci 2005, 6, 7-19.
- Lenahan M, O'brien S, Kinsella K, Sweeney T, Sheridan JJ. Prevalence and molecular characterization of Escherichia coli O157 : H7 on Irish lamb carcasses, fleece and in faeces samples. J Appl Microbiol 2007, 103, 2401-2409. https://doi.org/10.1111/j.1365-2672.2007.03476.x
- Mead PS, Griffin PM. Escherichia coli O157 : H7. Lancet 1998, 352, 1207-1212. https://doi.org/10.1016/S0140-6736(98)01267-7
- Naylor SW, Gally DL, Low JC. Enterohaemorrhagic E. coli in veterinary medicine. Int J Med Microbiol 2005, 295, 419-441. https://doi.org/10.1016/j.ijmm.2005.07.010
- Synge BA. Veterinary significance of verocytotoxin-producing Escherichia coli O157. World J Microbiol Biotechnol 2000, 16, 725-732. https://doi.org/10.1023/A:1008980923261