Comparative study of Clostridium perfringens, Salmonella spp. and E. coli focused on characteristics of E. coli O157 isolated from pigs of HACCP- and non-HACCP-accredited swine farms in Korea

  • Keum, Hyun Ok (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Kim, Hye Kwon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Rho, Se Mi (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Moon, Hyoung Joon (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Park, Seong Jun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Park, Bong Kyun (College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
  • Accepted : 2010.03.05
  • Published : 2010.06.30

Abstract

To determine the prevalence of Escherichia (E.) coli O157 : H7 from pigs after the Hazard Analysis and Critical Control Point (HACCP) system has been applied to Korean swine farm since 2006, 291 fecal samples were tested between May and December in 2008. Four E. coli O157:non-H7 (1.4%) were isolated from 4 different non-HACCP-accredited farms and they didn't have virulent genes which can cause illness for human. Also, Clostridium (C.) perfringens, Salmonella spp. and E. coli enterotoxins were tested using multiplex PCR. The positive rate for these pathogens of non-HACCP-accredited farms was higher than that of HACCP-accredited farms, and especially in case of C. perfringens, E. coli enterotoxins LT and STa, it was statistically significant (p < 0.05). Thus, the early implementation of the HACCP program is expected to greatly contribute to the safety of livestock products as well as food hygiene.

Acknowledgement

Supported by : Rural Development Administration

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