Evaluation of in vitro and in vivo bacteriophage efficacy against Salmonella enterica serovar Enteritidis infection

Bacteriophage의 Salmonella enterica serovar Enteritidis에 대한 in vitro 및 in vivo 효능 평가

  • Cha, Seung-Bin (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Rayamajhi, Nabin (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Lee, Won-Jung (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Shin, Min-Kyoung (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Roh, Yu-Mi (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Jung, Myung-Hwan (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University) ;
  • Myoung, Kil-Sun (R&D center, Korea Yakult) ;
  • Ahn, Young-Tae (R&D center, Korea Yakult) ;
  • Huh, Chul-Sung (R&D center, Korea Yakult) ;
  • Yoo, Han Sang (College of Veterinary Medicine, BK 21 for Veterinary Science and KRF Zoonotic Priority Research Institute, Seoul National University)
  • 차승빈 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소) ;
  • ;
  • 이원정 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소) ;
  • 신민경 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소) ;
  • 노유미 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소) ;
  • 정명환 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소) ;
  • 명길선 (한국 야쿠르트 R&D Center) ;
  • 안영태 (한국 야쿠르트 R&D Center) ;
  • 허철성 (한국 야쿠르트 R&D Center) ;
  • 유한상 (서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 중점연구소)
  • Accepted : 2010.06.29
  • Published : 2010.09.30

Abstract

Salmonella (S.) Enterica infection ranks among the most common food borne bacterial infections worldwide. Although there are six subspecies of S. Enterica, the vast majority of human and animal infections are caused by strains belonging to subspecies 1 serovar Typhimurium and Enteritidis. Recent reports on antibiotic resistance of Salmonella spp. are rising steadily. The increasing problem of antibiotic resistance has rekindled interest in bacteriophage to therapy. Therefore, we investigated the efficacy of bacteriophage in S. enterica serovar Enteritidis infected mice and pigs by measuring of body condition, body weight, bacterial colonization and weight of organs based on the in vitro analysis. In vitro experiment, phage cultured with S. Enteritidis showed clear lysis pattern, the plaque forming unit (PFU) of our phage culture was $1.5{\times}10^{11}PFU/mL$, and phage showed its maximum activity at 4 h post inoculation. In mouse experiment, there was no significant difference among experimental groups in the general body conditions and body weight of mice. However, there was difference in weight of liver and spleen depending on the experimental group (p < 0.05). The weight of liver and spleen were reduced by the phage treatment. Also bacterial colonization in spleen and liver were significantly reduced by the phage treatment. In pig experiment, the general body conditions and body temperature exhibited not much difference among the pigs except few pigs in group 3 which showed poor body conditions. From the feces in each group, we could isolate the S. Enteritidis only from group 3. Bacterial enrichment culture was necessary for isolating the bacteria from 5 dpi and 10 dpi, however direct isolation was possible from 15 dpi feces. In phage treated group, postmortem lesion was better than non-phage treated group. Recently, antibiotic resistance concerns on the food-borne bacterial pathogens have been increasing because of the wide spread of the antibiotics resistance genes. This concern is widely transmitted to the human related public health. As one of the alternative treatments on the bacterial pathogens, attempt using phages have been made to control the bacterial diseases. The positive possibility of the trail using phage was observed to control the S. enterica serovar Enteritidis in this study even though the further analysis has been remained.

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