Polymorphisms of the poly(C)-tract of porcine encephalomyocarditis virus (EMCV) isolated in Korea

국내분리 돼지 뇌심근염바이러스의 poly(C)-tract의 다형태성

  • Hyun, Bang-Hun (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Hyo-Jin (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, In-Joong (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Pyo, Hyun-Mi (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Sun-Mi (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Seong-Hee (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Jae-Jo (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Lim, Seong-In (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Song, Jae-Young (Virology Department, National Veterinary Research and Quarantine Service)
  • 현방훈 (국립수의과학검역원 바이러스과) ;
  • 김효진 (국립수의과학검역원 바이러스과) ;
  • 김인중 (국립수의과학검역원 바이러스과) ;
  • 표현미 (국립수의과학검역원 바이러스과) ;
  • 김선미 (국립수의과학검역원 바이러스과) ;
  • 김성희 (국립수의과학검역원 바이러스과) ;
  • 김재조 (국립수의과학검역원 바이러스과) ;
  • 임성인 (국립수의과학검역원 바이러스과) ;
  • 송재영 (국립수의과학검역원 바이러스과)
  • Accepted : 2010.07.02
  • Published : 2010.09.30

Abstract

Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.

Acknowledgement

Supported by : 국립수의과학검역원

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