Polymorphisms of the poly(C)-tract of porcine encephalomyocarditis virus (EMCV) isolated in Korea

국내분리 돼지 뇌심근염바이러스의 poly(C)-tract의 다형태성

  • Hyun, Bang-Hun (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Hyo-Jin (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, In-Joong (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Pyo, Hyun-Mi (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Sun-Mi (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Seong-Hee (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Kim, Jae-Jo (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Lim, Seong-In (Virology Department, National Veterinary Research and Quarantine Service) ;
  • Song, Jae-Young (Virology Department, National Veterinary Research and Quarantine Service)
  • 현방훈 (국립수의과학검역원 바이러스과) ;
  • 김효진 (국립수의과학검역원 바이러스과) ;
  • 김인중 (국립수의과학검역원 바이러스과) ;
  • 표현미 (국립수의과학검역원 바이러스과) ;
  • 김선미 (국립수의과학검역원 바이러스과) ;
  • 김성희 (국립수의과학검역원 바이러스과) ;
  • 김재조 (국립수의과학검역원 바이러스과) ;
  • 임성인 (국립수의과학검역원 바이러스과) ;
  • 송재영 (국립수의과학검역원 바이러스과)
  • Accepted : 2010.07.02
  • Published : 2010.09.30


Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.


Supported by : 국립수의과학검역원


  1. 박남용, 정치영, 이창영, 기혜영, 배성열, 이봉주, 하용공, 윤석민, 정병탁, 김동성. 번식장애를 수반한 돼지의 뇌심근염 바이러스 감염증. 대한수의학회지 1990, 30, 441-446.
  2. 박남용, 권영방, 윤지병, 안수환, 김병한, 권창희, 이재진, 강문일, 정병학, 하용공. 돼지 뇌심근염바이러스 감염증의 백신 개발. 과학기술처 특정연구개발사 업 연구보고서. 과학기술처, 서울, 1992.
  3. 하용공, 윤석민, 정병탁, 박남용, 이봉주, 정치영, 기혜영, 배성열. 돼지 뇌심근염 바이러스의 분리 배양. 대한수의학회 1991, 31, 479-484.
  4. Acland HM, Littlejohns IR. Encephalomyocarditis virus infection of pigs. 1. An outbreak in New South Wales. Aust Vet J 1975, 51, 409-415. https://doi.org/10.1111/j.1751-0813.1975.tb15789.x
  5. An DJ, Jeong W, Jeoung HY, Yoon SH, Kim HJ, Choi CU, Park BK. Encephalomyocarditis in Korea: serological survey in pigs and phylogenetic analysis of two historical isolates. Vet Microbiol 2009, 137, 37-44. https://doi.org/10.1016/j.vetmic.2009.01.005
  6. Canelli E, Luppi A, Lavazza A, Lelli D, Sozzi E, Martin AM, Gelmetti D, Pascotto E, Sandri C, Magnone W, Cordioli P. Encephalomyocarditis virus infection in an Italian zoo. Virol J 2010, 7, 64. https://doi.org/10.1186/1743-422X-7-64
  7. Cerutis DR, Bruner RH, Thomas DC, Giron DJ. Tropism and histopathology of the D, B, K, and MM variants of encephalomyocarditis virus. J Med Virol 1989, 29, 63-69. https://doi.org/10.1002/jmv.1890290112
  8. Cui T, Sankar S, Porter AG. Binding of encephalomyocarditis virus RNA polymerase to the 3'- noncoding region of the viral RNA is specific and requires the 3'-poly(A) tail. J Biol Chem 1993, 268, 26093-26098.
  9. Denis P, Liebig HD, Nowotny N, Billinis C, Papadopoulos O, O'Hara RS, Knowles NJ, Koenen F. Genetic variability of encephalomyocarditis virus (EMCV) isolates. Vet Microbiol 2006, 113, 1-12. https://doi.org/10.1016/j.vetmic.2005.10.032
  10. Duke GM, Hoffman MA, Palmenberg AC. Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation. J Virol 1992, 66, 1602-1609.
  11. Duke GM, Osorio JE, Palmenberg AC. Attenuation of Mengo virus through genetic engineering of the 5' noncoding poly(C) tract. Nature 1990, 343, 474-476. https://doi.org/10.1038/343474a0
  12. Gainer JH. Encephalomyocarditis virus infections in Florida, 1960-1966. J Am Vet Med Assoc 1967, 151, 421-425.
  13. Hahn H, Palmenberg AC. Encephalomyocarditis viruses with short poly(C) tracts are more virulent than their mengovirus counterparts. J Virol 1995, 69, 2697- 2699.
  14. Jeoung HY, Lee WH, Jeong W, Ko YJ, Choi CU, An DJ. Immune responses and expression of the viruslike particle antigen of the porcine encephalomyocarditis virus. Res Vet Sci 2010, 89, 295-300. https://doi.org/10.1016/j.rvsc.2010.03.012
  15. Joo HS. Encephalomyocarditis virus. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DJ (eds.). Diseases of Swine. 8th ed. pp. 139-144, Iowa State University Press, Ames, 1999.
  16. Kassimi LB, Boutrouille A, Gonzague M, Mbanda AL, Cruciere C. Nucleotide sequence and construction of an infectious cDNA clone of an EMCV strain isolated from aborted swine fetus. Virus Res 2002, 83, 71-87. https://doi.org/10.1016/S0168-1702(01)00425-7
  17. Kim HS, Christianson WT, Joo HS. Pathogenic properties of encephalomyocarditis virus isolates in swine fetuses. Arch Virol 1989, 109, 51-57. https://doi.org/10.1007/BF01310517
  18. Koenen F. Encephalomyocarditis virus. In: Straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ (eds.). Diseases of Swine. 9th ed. pp. 331-336, Iowa State University Press, Ames, 2006.
  19. Koenen F, De Clercq K, Lefebvre J, Strobbe R. Reproductive failure in sows following experimental infection with a Belgian EMCV isolate. Vet Microbiol 1994, 39, 111-116. https://doi.org/10.1016/0378-1135(94)90091-4
  20. LaRue R, Myers S, Brewer L, Shaw DP, Brown C, Seal BS, Njenga MK. A wild-type porcine encephalomyocarditis virus containing a short poly(C) tract is pathogenic to mice, pigs, and cynomolgus macaques. J Virol 2003, 77, 9136-9146. https://doi.org/10.1128/JVI.77.17.9136-9146.2003
  21. Martin LR, Duke GM, Osorio JE, Hall DJ, Palmenberg AC. Mutational analysis of the mengovirus poly(C) tract and surrounding heteropolymeric sequences. J Virol 1996, 70, 2027-2031.
  22. Martin LR, Neal ZC, McBride MS, Palmenberg AC. Mengovirus and encephalomyocarditis virus poly(C) tract lengths can affect virus growth in murine cell culture. J Virol 2000, 74, 3074-3081. https://doi.org/10.1128/JVI.74.7.3074-3081.2000
  23. Murnane TG, Craighead JE, Mondragon H, Shelokov A. Fatal disease of swine due to encephalomyocarditis virus. Science 1960, 131, 698-499.
  24. Osorio JE, Martin LR, Palmenberg AC. The immunogenic and pathogenic potential of short poly(C) tract Mengo viruses. Virology 1996, 223, 344-350. https://doi.org/10.1006/viro.1996.0485
  25. Palmenberg AC, Kirby EM, Janda MR, Drake NL, Duke GM, Potratz KF, Collett MS. The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Res 1984, 12, 2969-2985. https://doi.org/10.1093/nar/12.6.2969
  26. Paschaleri-Papadopoulou E, Axiotis I, Laspidis C. Encephalomyocarditis of swine in Greece. Vet Rec 1990, 126, 364-365.
  27. Petruccelli MA, Hirasawa K, Takeda M, Itagaki S, Doi K. Cardiac and pancreatic lesions in guinea pigs infected with encephalomyocarditis (EMC) virus. Histol Histopathol 1991, 6, 167-170.
  28. Ramos JK, Contero W, Royna A, Guterrz R. Encephalomyocarditis viral porcina. Considerations clinicas y anatomopathologicas de un brote. Rev Cubana Cienc Vet 1982, 13, 47-52
  29. Song MS, Joo Y, Lee EH, Shin JY, Kim CJ, Shin KS, Sung MH, Choi YK. Genetic characterization of encephalomyocarditis virus isolated from aborted swine fetus in Korea. J Microbiol Biotechnol 2006, 16, 1570-1576.
  30. Sutherland RJ, Horner GW, Hunter R, Fyfe BH. An outbreak of viral Encephalomyocarditis in pig. NZ Vet J 1977, 25, 225. https://doi.org/10.1080/00480169.1977.34412
  31. Williams MC. Encephalomyocarditis virus infection. J South Afr Vet Assoc 1981, 52, 76.
  32. Zhang GQ, Ge XN, Guo X, Yang HC. Genomic analysis of two porcine encephalomyocarditis virus strains isolated in China. Arch Virol 2007, 152, 1209-1213. https://doi.org/10.1007/s00705-006-0930-9
  33. Zimmermann A, Nelsen-Salz B, Kruppenbacher JP, Eggers HJ. The complete nucleotide sequence and construction of an infectious cDNA clone of a highly virulent encephalomyocarditis virus. Virology 1994, 203, 366-372. https://doi.org/10.1006/viro.1994.1495