Effect of water extract of Danshen on bone regeneration of rat calvarial defect model

랫드 두개골 결손부에서 단삼 수용성 추출물의 골형성 효과

  • Shim, Kyung Mi (Department of Radiology, Nambu University) ;
  • Kim, Se Eun (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Seong Soo (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2010.07.09
  • Published : 2010.09.30

Abstract

The purpose of this study was to evaluate the osteogenic capacity of water extract of danshen (Salvia miltiorrhiza Bunge). We have established in rat critical-sized calvarial defect model using the combination with collagen scaffold and danshen hydrophilic extract. All rats were extinguished at 8 weeks after bone graft surgery, and the bone regeneration ability of bone grafting sides was evaluated by plain radiography and micro-CT. These results revealed water extract of danshen had the potential to promote osteogenesis especially continuous oral administration with local treatment compared to one-shot local treatment. This compound may provide a new alternative agent for growth factors to promote bone healing and bone regeneration. In conclusion, these results suggest that danshen hydrophilic extract have the potential to promote osteogenesis in bone defects. Further studies about fusion technology with salvianolic acid B, peptides, growth factors, and scaffolds using of the combination of tissue engineering, cell engineering and mechanical engineering are needed.

Acknowledgement

Supported by : 한국연구재단

References

  1. Bolder SB, Schreurs BW, Verdonschot N, van Unen JM, Gardeniers JW, Slooff TJ. Particle size of bone graft and method of impaction affect initial stability of cemented cups: human cadaveric and synthetic pelvic specimen studies. Acta Orthop Scand 2003, 74, 652-657. https://doi.org/10.1080/00016470310018144
  2. Boyce T, Edwards J, Scarborough N. Allograft bone. The influence of processing on safety and performance. Orthop Clin North Am 1999, 30, 571-581. https://doi.org/10.1016/S0030-5898(05)70110-3
  3. Chae HJ, Chae SW, Yun DH, Keum KS, Yoo SK, Kim HR. Prevention of bone loss in ovariectomized rats: the effect of Salvia miltiorrhiza extracts. Immunopharmacol Immunotoxicol 2004, 26, 135-144. https://doi.org/10.1081/IPH-120029951
  4. Chang PN, Mao JC, Huang SH, Ning L, Wang ZJ, On T, Duan W, Zhu YZ. Analysis of cardioprotective effects using purified Salvia miltiorrhiza extract on isolated rat hearts. J Pharmacol Sci 2006, 101, 245-249. https://doi.org/10.1254/jphs.FPJ05034X
  5. Cheng TO. Cardiovascular effects of Danshen. Int J Cardiol 2007, 121, 9-22. https://doi.org/10.1016/j.ijcard.2007.01.004
  6. Clayman L. Implant reconstruction of the bone-grafted maxilla: review of the literature and presentation of 8 cases. J Oral Maxillofac Surg 2006, 64, 674-682. https://doi.org/10.1016/j.joms.2005.12.026
  7. Clokie CM, Sndor GK. Reconstruction of 10 major mandibular defects using bioimplants containing BMP- 7. J Can Dent Assoc 2008, 74, 67-72.
  8. de Boer HH. The history of bone grafts. Clin Orthop Relat Res 1988, 226, 292-298.
  9. Ding Y, Soma S, Takano-Yamamoto T, Matsumoto S, Sakuda M. Effects of salvia miltiorrhiza bunge (SMB) on MC3T3-E1 cells. J Osaka Univ Dent Sch 1995, 35, 21-27.
  10. Dunlop DG, Brewster NT, Madabhushi SP, Usmani AS, Pankaj P, Howie CR. Techniques to improve the shear strength of impacted bone graft: the effect of particle size and washing of the graft. J Bone Joint Surg Am 2003, 85-A, 639-646.
  11. Goldberg VM, Stevenson S. The biology of bone grafts. Semin Arthroplasty 1993, 4, 58-63.
  12. Hayden RJ, Jebson PJ. Wrist arthrodesis. Hand Clin 2005, 21, 631-640. https://doi.org/10.1016/j.hcl.2005.08.004
  13. Hernigou P, Poignard A, Manicom O, Mathieu G, Rouard H. The use of percutaneous autologous bone marrow transplantation in nonunion and avascular necrosis of bone. J Bone Joint Surg Br 2005, 87, 896-902. https://doi.org/10.1302/0301-620X.87B7.16289
  14. Huang X, Jiang H, Liu D, Zhou Z, Wang L. Implantation of calcium phosphate cement/Danshen drug delivery system for avascular necrosis of femoral head. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2008, 22, 307-310.
  15. Kang DG, Oh H, Chung HT, Lee HS. Inhibition of angiotensin converting enzyme by lithospermic acid B isolated from Radix Salviae miltiorrhiza Bunge. Phytother Res 2003, 17, 917-920. https://doi.org/10.1002/ptr.1250
  16. Kim HK, Woo ER, Lee HW, Park HR, Kim HN, Jung YK, Choi JY, Chae SW, Kim HR, Chae HJ. The correlation of Salvia miltiorrhiza extract-induced regulation of osteoclastogenesis with the amount of components tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone. Immunopharmacol Immunotoxicol 2008, 30, 347-364. https://doi.org/10.1080/08923970801949133
  17. Kraus KH, Kirker-Head C. Mesenchymal stem cells and bone regeneration. Vet Surg 2006, 35, 232-242. https://doi.org/10.1111/j.1532-950X.2006.00142.x
  18. Kwak HB, Yang D, Ha H, Lee JH, Kim HN, Woo ER, Lee S, Kim HH, Lee ZH. Tanshinone IIA inhibits osteoclast differentiation through down-regulation of c- Fos and NFATc1. Exp Mol Med 2006, 38, 256-264. https://doi.org/10.1038/emm.2006.31
  19. Linkhart TA, Mohan S, Baylink DJ. Growth factors for bone growth and repair: IGF, TGF beta and BMP. Bone 1996, 19 (Suppl), 1S-12S. https://doi.org/10.1016/S8756-3282(96)00138-X
  20. Liu YR, Qu SX, Maitz MF, Tan R, Weng J. The effect of the major components of Salvia Miltiorrhiza Bunge on bone marrow cells. J Ethnopharmacol 2007, 111, 573-583. https://doi.org/10.1016/j.jep.2007.01.005
  21. Meijer GJ, de Bruijn JD, Koole R, van Blitterswijk CA. Cell-based bone tissue engineering. PLoS Med 2007, 4, e9. https://doi.org/10.1371/journal.pmed.0040009
  22. Munk B, Larsen CF. Bone grafting the scaphoid nonunion: a systematic review of 147 publications including 5,246 cases of scaphoid nonunion. Acta Orthop Scand 2004, 75, 618-629. https://doi.org/10.1080/00016470410001529
  23. Nishida J, Shimamura T. Methods of reconstruction for bone defect after tumor excision: a review of alternatives. Med Sci Monit 2008, 14, RA107-113.
  24. O K, Cheung F, Sung FL, Zhu DY, Siow YL. Effect of magnesium tanshinoate B on the production of nitric oxide in endothelial cells. Mol Cell Biochem 2000, 207, 35-39. https://doi.org/10.1023/A:1007081911734
  25. Pilitsis JG, Lucas DR, Rengachary SS. Bone healing and spinal fusion. Neurosurg Focus 2002, 13, e1.
  26. Safran O, Iannotti JP. Arthrodesis of the shoulder. J Am Acad Orthop Surg 2006, 14, 145-153. https://doi.org/10.5435/00124635-200603000-00005
  27. Sakou T. Bone morphogenetic proteins: from basic studies to clinical approaches. Bone 1998, 22, 591-603. https://doi.org/10.1016/S8756-3282(98)00053-2
  28. Sauer HD, Schoettle H. The stability of osteosyntheses bridging defects. Arch Orthop Trauma Surg 1979, 95, 27-30. https://doi.org/10.1007/BF00379166
  29. Shi H, Ma J, Zhao N, Chen Y, Liao Y. Periodontal regeneration in experimentally-induced alveolar bone dehiscence by an improved porous biphasic calcium phosphate ceramic in beagle dogs. J Mater Sci Mater Med 2008, 19, 3515-3524. https://doi.org/10.1007/s10856-008-3524-0
  30. Shim KM, Kim SE, Yoo KH, Bae CS, Choi SH, Kang SS. Case studies of repair of pathological mandibular fracture due to periodontal disease in dogs. J Vet Clin 2007, 24, 653-657.
  31. Shors EC. Coralline bone graft substitutes. Orthop Clin North Am 1999, 30, 599-613. https://doi.org/10.1016/S0030-5898(05)70113-9
  32. Tiwana PS, Kushner GM, Haug RH. Maxillary sinus augmentation. Dent Clin North Am 2006, 50, 409-424. https://doi.org/10.1016/j.cden.2006.03.004
  33. Wong RW, Rabie AB. Effect of Salvia miltiorrhiza extract on bone formation. J Biomed Mater Res A 2008, 85, 506-512.
  34. Wong RW, Rabie AB. Traditional Chinese medicines and bone $formation^{\circ\;TM}$ a review. J Oral Maxillofac Surg 2006, 64, 828-837. https://doi.org/10.1016/j.joms.2006.01.017
  35. Zhao GR, Zhang HM, Ye TX, Xiang ZJ, Yuan YJ, Guo ZX, Zhao LB. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B. Food Chem Toxicol 2008, 46, 73-81. https://doi.org/10.1016/j.fct.2007.06.034
  36. Zhou L, Zuo Z, Chow MS. Danshen: an overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J Clin Pharmacol 2005, 45, 1345-1359. https://doi.org/10.1177/0091270005282630