Evaluation of character on lymphocyte subpopulations from peripheral blood in Hanwoo and Holstein neonatal calves

한우와 젖소 신생 송아지의 말초혈액 림프구아군 특성비교

  • Jung, Young-Hun (National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Seog-Jin (National Institute of Animal Science, Rural Development Administration) ;
  • Ki, Kwang-Seok (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Sung-Jae (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Myeung-Sik (National Institute of Animal Science, Rural Development Administration) ;
  • Suh, Guk-hyun (College of Veterinary Medicine, Chonnam National University)
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 강석진 (농촌진흥청 국립축산과학원) ;
  • 기광석 (농촌진흥청 국립축산과학원) ;
  • 박성재 (농촌진흥청 국립축산과학원) ;
  • 이명식 (농촌진흥청 국립축산과학원) ;
  • 서국현 (전남대학교 수의과대학)
  • Accepted : 2010.08.04
  • Published : 2010.09.30


The present study was undertaken to establish reference values for the composition blood lymphocyte populations and compare forty three Hanwoo neonatal calves (KC) with twenty one Holstein calves (HC) by blood cell count and immunophynotying. The percentages of CD2+, CD4+, CD8+, CD26+, ACT2+, MHC class, MHC class II and WC1+ T cells, B cells were determined by flow cytometry. The number of lymphocyte and monocyte in HC were higher than those of KC. However, the number of neutrophils was higher in HC than KC. The proportions of CD2+, CD4+, CD8+, MHC class, and WC1+ lymphocytes remained relatively stable during the study period, while there was a moderate increase in the relative percentage of CD26+, ACT2+, MHC class II and B cell from birth to approximately 3 weeks of age. Marked differences in the relative proportions of the lymphocyte subpopulations were noted between the individual calves. The present study shows that the T-cell subpopulations are present in peripheral blood of KC at levels comparable with HC, while the MHC class II and B cell population of KC increases significantly with age. The absolute number of WBC in KC was due to the decrease of absolute number of neutrophil rather than the increase of lymphocyte. The results indicated that KC have significantly higher number of neutrophils, and proportion of MHC class II and B cell than HC.


  1. 김두, 한홍율. 한우 송아지의 초유섭취에 의한 수동면역 획득 상태. 대한수의학회지 1989, 29, 75-81.
  2. 김두, 한홍율. 한우 송아지의 초유섭취에 의한 수동면역이 포유기간 중의 질병발생에 미치는 영향. 대한수의학회지 1989, 29, 91-98.
  3. 김정기, 장국현, 김태종, 윤화중. 강원도 지역 한우의 혈액상에 관한 연구. 대한수의사회지 1989, 25, 102-107.
  4. 문진산, 박용호, 정석찬, 구복경, 강병규. 백혈구 표면항원 특이 단크론항체를 이용한 한우의 말초혈액 백혈구 아군에 대한 연구. 대한수의학회지 1996, 36, 337-348.
  5. 류경표, 이경갑. 포유기 송아지의 혈액화학치의 변화. 한국임상수의학회지 1997, 14, 201-207.
  6. 정창국. 한국성우의 혈액학치 및 혈액화학치에 관한 연구. 대한수의학회지 1965, 5, 61-96.
  7. Adams R, Garry FB, Aldridge BM, Holland MD, Odde KG. Hematologic values in newborn beef calves. Am J Vet Res 1992, 53, 944-950.
  8. Ayoub IA, Yang TJ. Age-dependent changes in peripheral blood lymphocyte subpopulations in cattle: a longitudinal study. Dev Comp Immunol 1996, 20, 353-363. https://doi.org/10.1016/S0145-305X(96)00024-9
  9. Brun-Hansen HC, Kampen AH, Lund A. Hematologic values in calves during the first 6 months of life. Vet Clin Pathol 2006, 35, 182-187. https://doi.org/10.1111/j.1939-165X.2006.tb00111.x
  10. Clevers H, MacHugh ND, Bensaid A, Dunlap S, Baldwin CL, Kaushal A, Iams K, Howard CJ, Morrison WI. Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor $\gamma/\delta^{+}$ T lymphocytes. Eur J Immunol 1990, 20, 809-817. https://doi.org/10.1002/eji.1830200415
  11. Davis WC, Marusic S, Lewin HA, Splitter GA, Perryman LE, McGuire TC, Gorham JR. The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol 1987, 15, 337-376. https://doi.org/10.1016/0165-2427(87)90005-5
  12. Holman HH. The blood picture of the cow. Br Vet J 1955, 111, 440. https://doi.org/10.1016/S0007-1935(17)47004-7
  13. Jain NC. Schalm's Veterinary Hematology. 4th ed. pp. 98-99, Lea & Febiger, Philadelphia. 1986.
  14. Kampen AH, Olsen I, Tollersrud T, Storset AK, Lund A. Lymphocyte subpopulations and neutrophil function in calves during the first 6 months of life. Vet Immunol Immunopathol 2006, 113, 53-63. https://doi.org/10.1016/j.vetimm.2006.04.001
  15. Knowles TG, Edwards JE, Bazeley KJ, Brown SN, Butterworth A, Warriss PD. Changes in the blood biochemical and haematological profile of neonatal calves with age. Vet Rec 2000, 147, 593-598. https://doi.org/10.1136/vr.147.21.593
  16. Kulberg S, Boysen P, Storset AK. Reference values for relative numbers of natural killer cells in cattle blood. Dev Comp Immunol 2004, 28, 941-948. https://doi.org/10.1016/j.dci.2004.02.004
  17. Menge C, Neufeld B, Hirt W, Bauerfeind R, Baljer G, Wieler LH. Phenotypical characterization of peripheral blood leucocytes in the newborn calf. Zentralbl Veterinarmed B 1999, 46, 559-565.
  18. Mohri M, Sharifi K, Eidi S. Hematology and serum biochemistry of Holstein dairy calves: age related changes and comparison with blood composition in adults. Res Vet Sci 2007, 83, 30-39. https://doi.org/10.1016/j.rvsc.2006.10.017
  19. Ohtsuka H, Fukunaga N, Fukuda S, Hatsugaya A, Hayashi T, Hara H, Koiwa M, Abe R, Kawamura S. Effect of nutritional conditions on changes in leukocyte populations in Japanese black calves. J Vet Med Sci 2005, 67, 183-185. https://doi.org/10.1292/jvms.67.183
  20. Ohtsuka H, Fukunaga N, Hara H, Fukuda S, Hayashi T, Hoshi F, Yoshino TO, Koiwa M, Kawamura S. Changes in peripheral leukocyte populations of weak calf syndrome of Japanese black calves. J Vet Med Sci 2003, 65, 793-796. https://doi.org/10.1292/jvms.65.793
  21. Park YH, Fox LK, Hamilton MJ, Davis WC. Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation. J Dairy Sci 1992, 75, 998-1006. https://doi.org/10.3168/jds.S0022-0302(92)77842-4
  22. Pollock JM, Welsh MD. The $WC1^{+}$ $\gamma\delta$ T-cell population in cattle: a possible role in resistance to intracellular infection. Vet Immunol Immunopathol 2002, 89, 105-114. https://doi.org/10.1016/S0165-2427(02)00200-3
  23. Suh GH, Hur TY, Son DS, Choe CY, Jung YH, Ahn BS, Lee CY, Lee CG. Differences in the serum immunoglobulin concentrations between dairy and beef calves from birth to 14 days of age. J Vet Sci 2003, 4, 257-260.
  24. Tizard IR. Veterinary Immunology: An Introduction. pp. 98-103, Saunders, Philadelphia, 2004.
  25. Wilson RA, Zolnai A, Rudas P, Frenyo LV. T-cell subsets in blood and lymphoid tissues obtained from fetal calves, maturing calves, and adult bovine. Vet Immunol Immunopathol 1996, 53, 49-60. https://doi.org/10.1016/0165-2427(95)05543-6
  26. Wyatt CR, Madruga C, Cluff C, Parish S, Hamilton MJ, Goff W, Davis WC. Differential distribution of gamma delta T-cell receptor lymphocyte subpopulations in blood and spleen of young and adult cattle. Vet Immunol Immunopathol 1994, 40, 187-199. https://doi.org/10.1016/0165-2427(94)90019-1