Evaluation of character on lymphocyte subpopulations from peripheral blood in Hanwoo and Holstein neonatal calves

한우와 젖소 신생 송아지의 말초혈액 림프구아군 특성비교

  • Jung, Young-Hun (National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Seog-Jin (National Institute of Animal Science, Rural Development Administration) ;
  • Ki, Kwang-Seok (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Sung-Jae (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Myeung-Sik (National Institute of Animal Science, Rural Development Administration) ;
  • Suh, Guk-hyun (College of Veterinary Medicine, Chonnam National University)
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 강석진 (농촌진흥청 국립축산과학원) ;
  • 기광석 (농촌진흥청 국립축산과학원) ;
  • 박성재 (농촌진흥청 국립축산과학원) ;
  • 이명식 (농촌진흥청 국립축산과학원) ;
  • 서국현 (전남대학교 수의과대학)
  • Accepted : 2010.08.04
  • Published : 2010.09.30

Abstract

The present study was undertaken to establish reference values for the composition blood lymphocyte populations and compare forty three Hanwoo neonatal calves (KC) with twenty one Holstein calves (HC) by blood cell count and immunophynotying. The percentages of CD2+, CD4+, CD8+, CD26+, ACT2+, MHC class, MHC class II and WC1+ T cells, B cells were determined by flow cytometry. The number of lymphocyte and monocyte in HC were higher than those of KC. However, the number of neutrophils was higher in HC than KC. The proportions of CD2+, CD4+, CD8+, MHC class, and WC1+ lymphocytes remained relatively stable during the study period, while there was a moderate increase in the relative percentage of CD26+, ACT2+, MHC class II and B cell from birth to approximately 3 weeks of age. Marked differences in the relative proportions of the lymphocyte subpopulations were noted between the individual calves. The present study shows that the T-cell subpopulations are present in peripheral blood of KC at levels comparable with HC, while the MHC class II and B cell population of KC increases significantly with age. The absolute number of WBC in KC was due to the decrease of absolute number of neutrophil rather than the increase of lymphocyte. The results indicated that KC have significantly higher number of neutrophils, and proportion of MHC class II and B cell than HC.

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