Biological activities and single oral dose toxicity in rat of fermented Rhus verniciflua extract

발효 옻 추출물의 생리활성 및 단회 경구 투여 독성시험

  • Choi, Myung-Jin (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Seung-Jin (College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, Seung-Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Reza, Md. Ahsanur (College of Veterinary Medicine, Kyungpook National University) ;
  • Hong, Joo-Heon (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Jung, Hee-Kyoun (Bio Industry Center, Daegu Technopark) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • 최명진 (경북대학교 수의과대학) ;
  • 이승진 (경북대학교 수의과대학) ;
  • 장승희 (경북대학교 수의과대학) ;
  • ;
  • 홍주헌 (대구가톨릭대학교 외식식품산업학부) ;
  • 정희경 (대구테크노파크 바이오산업지원센터) ;
  • 박승춘 (경북대학교 수의과대학)
  • Accepted : 2010.07.27
  • Published : 2010.09.30

Abstract

In this study, we investigated the biological activities such as anti-tumor, anti-oxidant and antiinflammatory activities as well as single oral dose toxicity of fermented Rhus verniciflua extract (FRVE). In order to examine anti-tumor activity of FRVE, the sarcoma 180 cells were treated with FRVE at various concentrations (0.03, 0.3, 3 and 30 mg/mL) in microtetrazolium (MTT) assay. In MTT assay, all the cells treated with FRVE at various concentrations have shown a significant difference compared with control (p < 0.05). In xanthine oxidase inhibition assay to examine the antioxidant activity, the xanthine oxidase inhibition rate of FRVE at 1.5 mg/mL and 15 mg/mL was $85{\pm}15.01%$ and $99{\pm}16.02%$, respectively. Nitric oxide production in RAW 264.7 cells showed that FRVE showed a significant anti-inflammation effect at 3 mg/mL (p < 0.05). In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs, body weight gains, feed and water consumptions. The results indicated that lethal dose 50 ($LD_{50}$) of FRVE was found to be higher than 5,000 mg/kg in this experiment. From the above results, we may suggest that FRVE might have useful as a material for functional food and/or animal pharmaceutics.

Acknowledgement

Supported by : 한국산업단지공단

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