Characteristics and antimicrobial susceptibility of Escherichia coli isolated from horse

말에서 분리한 Escherichia coli의 특성 및 항생제 감수성

  • Yun, Sung-Wook (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Do-Yeon (Korea Food & Drug Administration) ;
  • Choi, Seong-kyoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Hee-Soo (National Veterinary Research & Quarantine Service) ;
  • Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2010.08.18
  • Published : 2010.09.30

Abstract

This study was conducted to investigate characteristics and antimicrobial susceptibility of Escherichia (E.) coli isolates isolated from vaginal mucosa and clitorial fossa of 105 Thoroughbred mares suspicious of the genital disease in Korea during the period from March 2006 to July 2007. Ninety six E. coli isolates were identified as standard biochemical properties and using BIOLOG system. Fifty three isolates (55.2%) could be classified into a total of 21 O serotypes and forty three isolates (44.8%) were non-typeable with 51 O antisera used in this study. The verotoxin 1 (VT 1) and verotoxin 2 genes were analyzed by multiplex PCR. Among them, one isolate was detected VT 1 gene (130 bp). Most of isolates showed a high susceptibility in ciprofloxacin (100%), enrofloxacin (100%), norfloxacin (100%), cefoxitin (96.9%), gentamicin (96.9%), sulphamethoxazole (96.9%), nitrofurantoin (94.8%), amikacin (93.8%), nalidixic acid (92.7%) and tetracycline (90.6%). These results may provide the basic information to establish strategies for the treatment and prevention of reproductive disease in Thoroughbred mares in Korea.

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