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Anti-oxidant and Anti-microbial Activities of Seungmakalgeuntang

복합처방인 승마갈근탕(升摩葛根湯)의 항산화 및 항균효과

  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Bae, Ho-Jung (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Tae-Soon (R&D Hanbang Cosmetic Team, Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Kim, Tae-Wan (Department of Food Science & Biotechnology, Andong National University) ;
  • Moon, Doo-Hwan (PioKorea Co., LTD.) ;
  • Kwon, O-Jun (Gyeongbuk Regional Innovation Agency) ;
  • Son, Jun-Ho (R&D Hanbang Cosmetic Team, Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Gun-Hye (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Han-Hyuk (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 이진영 (호서대학교 한방화장품과학과) ;
  • 배호정 (대구한의대학교 화장품약리학과) ;
  • 박태순 (대구경북한방산업진흥원 한방화장품팀) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 문두환 (피오코리아) ;
  • 권오준 (경북전략산업기획단) ;
  • 손준호 (대구경북한방산업진흥원 한방화장품팀) ;
  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 박근혜 (대구한의대학교 화장품약리학과) ;
  • 김한혁 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2009.05.13
  • Accepted : 2009.12.17
  • Published : 2010.03.31

Abstract

Biological activities such as anti-oxidative and anti-microbial of the Seungmakalgeuntang, a traditional prescription, were evaluated. The electron donating ability of water, ethanol, supercritical fluid and 1,3-butylene glycol extract of Seungmakalgeuntang showed more than 50% at a 100 ppm concentration. At a 1000 ppm concentration, the superoxide dismutase-like activities of ethanol and supercritical fluid extract of Seungmakalgeuntang showed less than 50%. xanthine oxidase inhibition effect of the supercritical fluid extract showed more than 70% at a 1,000 ppm concentration, which was higher than vitamin C. From the measurement on lipid oxidation, the $Fe^{2+}$ chelating abilities of the supercritical fluid extract of Seungmakalgeuntang was more than 60% at a 100 ppm concentration. Also the $Cu^{2+}$ chelating abilities of supercritical fluid extract Seungmkalgeuntang was showed more than 60% at a 500 ppm concentration. Clear zones formed by sample against the human skin-resident microflora such as Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acne of ethanol and supercritical fluid extract of Seungmakalgeuntang showed the highest among all the extracts tested using a 4mg/disc. The minimum inhibitory concentration (MIC) against both S. epidermidis and S. aureus showed 2,500 ppm in the extract of the supercritical fluid.

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