- Volume 37 Issue 6
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Analysis of Micronuclei and Its Association with Genetic Polymorphisms in Hospital Workers Exposed to Ethylene Oxide
에틸렌옥사이드(Ethylene oxide)에 노출된 병원 근로자들의 소핵 빈도와 유전적 감수성 지표와의 연관성
- Lee, Sun-Yeong ;
- Kim, Yang-Jee ;
- Choi, Young-Joo ;
- Lee, Joong-Won ;
- Lee, Young-Hyun ;
- Shin, Mi-Yeon ;
- Kim, Won ;
- Yoon, Chung-Sik ;
- Kim, Sung-Kyoon ;
- Chung, Hai-Won
- Received : 2011.12.07
- Accepted : 2011.12.28
- Published : 2011.12.31
Objectives: Ethylene oxide (EtO) is classified as a human carcinogen, but EtO is still widely used to sterilize heat-sensitive materials in hospitals. Employees working around sterilizers are exposed to EtO after sterilization. The aim of the present study was to assess the exposure of EtO level, coupled with occupationally induced micronuclei from hospital workers. The influence of genetic polymorphisms of detoxifying genes (GSTT1 and GSTM1) and DNA repair genes (XRCC1 and XRCC3) on the frequencies of micronuclei in relation to exposure of EtO was also investigated. Methods: The study population was composed of 35 occupationally exposed workers to EtO, 18 student controls and 44 unexposed hospital controls in Korea. Exposure to EtO is measured by passive personal samplers. We analyzed the frequencies of micronuclei by performing cytokinesis-block micronucleus assay (CBMN assay) and GSTM1, GSTT1, XRCC1, and XRCC3 were also genotyped by performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of micronuclei in EtO exposure group, student controls and hospital controls were
Ethylene oxide (EtO);Genetic polymorphism;Glutathione S-transferase (GST);Hospital workers;Micronuclei (MN);X-ray repair cross-complementing group (XRCC)
- Won JU. Health effects of chemicals used in hospitals among healthcare workers. J Korean Med Assoc. 2010; 53(6): 474-482. https://doi.org/10.5124/jkma.2010.53.6.474
- Lin YK, Lee LH, et al. An integrated occupational health consultation model for the medical supply manufacturing industry. Industrial Health. 2010; 48(6): 749-757. https://doi.org/10.2486/indhealth.MS1113
- Chang SH, Lee WJ, Kim CS, Hwang CH, Park JT, Kim DS, et al. Sister chromatid exchanges in workers occupationally exposed to ethylene oxide. Korean J Occup Environ Med. 1998; 10(4): 428- 437.
- Jeon HJ, Kim TH, Choi SY, Chung HW. Analysis of chromosome aberrations and micronuclei in peripheral lymphocytes of nurses in a hospital. Environmental Mutagens & Carcinogens. 2005; 25(4): 150-156.
- Segerback D. Reaction products in hemoglobin and DNA after in vitro treatment with ethylene oxide and N-(2-hydroxyethyl)-N-nitrosourea. Carcinogenesis. 1990; 11(2): 307-312. https://doi.org/10.1093/carcin/11.2.307
- Thier R and Bolt HM. Carcinogenicity and genotoxicity of ethylene oxide: new aspects and recent advances. Critical Reviews in Toxicology. 2000; 30(5): 595-608. https://doi.org/10.1080/10408440008951121
- Anonymous. Guide to occupational exposure values 2006. Cincinnati: ACGIH; 2006. p. 58.
- IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. IARC monographs on the evaluation of carcinogenic risks to humans. Volume 97. 1, 3-butadiene, ethylene oxide and vinyl halides (vinyl fluoride, vinyl chloride and vinyl bromide). IARC Monogr Eval Carcinog Risks Hum. 2008; 97: 3-471.
- Fenech M, Perepetskaya G, Mikhalevich L. A more comprehensive application of the micronucleus technique for biomonitoring of genetic damage rates in humanpopulations-experiences from the Chernobyl catastrophe. Environ. Mol. Mutagen. 1997; 30(2): 112-118. https://doi.org/10.1002/(SICI)1098-2280(1997)30:2<112::AID-EM3>3.0.CO;2-P
- Fenech M. The in vitro micronucleus technique. Mutat Res. 2000; 455(1-2): 81-95. https://doi.org/10.1016/S0027-5107(00)00065-8
- Mannervik B, Alin P, et al. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci U S A. 1985; 82(21): 7202-7206. https://doi.org/10.1073/pnas.82.21.7202
- Fennell TR, MacNeela JP, et al. Hemoglobin adducts from acrylonitrile and ethylene oxide in cigarette smokers: effects of glutathione S-transferase T1-null and M1-null genotypes. Cancer Epidemiol Biomarkers Prev. 2000; 9(7): 705-712.
- Agurell E, Cederberg H, et al. Genotoxic effects of ethylene oxide and propylene oxide: a comparative study. Mutat Res. 1991; 250(1-2): 229-237. https://doi.org/10.1016/0027-5107(91)90180-V
- Goode EL, Ulrich CM, et al. Polymorphisms in DNA repair genes and associations with cancer risk. Cancer Epidemiol Biomarkers Prev. 2002; 11(12): 1513-1530.
- Hagmar L, Welinder H, et al. An epidemiological study of cancer risk among workers exposed to ethylene oxide using hemoglobin adducts to validate environmental exposure assessments. Int Arch Occup Environ Health. 1991; 63(4): 271-277. https://doi.org/10.1007/BF00386377
- Fenech M, Chang WP, et al. HUMN project:detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res. 2003; 534(1-2): 65-75. https://doi.org/10.1016/S1383-5718(02)00249-8
- Roodi N, Dupont WD, et al. Association of homozygous wild-type glutathione S-transferase M1 genotype with increased breast cancer risk. Cancer Research. 2004; 64(4): 1233-1236. https://doi.org/10.1158/0008-5472.CAN-03-2861
- Schlade-Bartusiak K, Rozik K, et al. Influence of GSTT1, mEH, CYP2E1 and RAD51 polymorphisms on diepoxybutane-induced SCE frequency in cultured human lymphocytes. Mutat Res. 2004; 558(1-2): 121-130. https://doi.org/10.1016/j.mrgentox.2003.11.007
- Shen MR, Jones IM, et al. Nonconservative amino acid substitution variants exist at polymorphic frequency in DNA repair genes in healthy humans. Cancer Research. 1998; 58(4): 604-608.
- Winsey SL, Haldar NA, et al. A variant within the DNA repair gene XRCC3 is associated with the development of melanoma skin cancer. Cancer Research. 2000; 60(20): 5612-5616.
- Guo SW and Thompson EA. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics. 1992; 48(2): 361-372. https://doi.org/10.2307/2532296
- Catalan J, Autio K, Kuosma E, and H Norppa. Age-dependent inclusion of sex chromosomes in lymphocyte micronuclei of man. Am J Hum Genet. 1998; 63(5): 1464-1472. https://doi.org/10.1086/302092
- Lim HS, Ahn YS. Occupational diseases among health care workers approved by Korea Labor Welfare Corporation. Korean J Occup Environ Med. 2003; 15(2): 196-204.
- Ribeiro LR, Salvadori DM, et al. Biological monitoring of workers occupationally exposed to ethylene oxide. Mutat Res. 1994; 313(1): 81-87. https://doi.org/10.1016/0165-1161(94)90035-3
- Tates AD, Grummt T, et al. Biological and chemical monitoring of occupational exposure to ethylene oxide. Mutat Res. 1991; 250(1-2): 483-497. https://doi.org/10.1016/0027-5107(91)90205-3
- Sarto F, Tomanin R, et al. The micronucleus assay in human exfoliated cells of the nose and mouth: application to occupational exposures to chromic acid and ethylene oxide. Mutat Res. 1990; 244(4): 345-351. https://doi.org/10.1016/0165-7992(90)90083-V
- Garte S, Gaspari L, et al. Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomarkers Prev. 2001; 10(12): 1239- 1248.
- Hallier E, Langhof T, et al. Polymorphism of glutathione conjugation of methyl bromide, ethylene oxide and dichloromethane in human blood: influence on the induction of sister chromatid exchanges (SCE) in lymphocytes. Archives of Toxicology. 1993; 67(3): 173-178. https://doi.org/10.1007/BF01973304
- Hayes JD and Pulford DJ The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995; 30(6): 445-600. https://doi.org/10.3109/10409239509083491
- Cho YH, Kim YJ, et al. Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers. Mutat Res. 2009; 680(1-2): 17-24 https://doi.org/10.1016/j.mrgentox.2009.08.007
- Godderis L, Aka P, et al. Dose-dependent influence of genetic polymorphisms on DNA damage induced by styrene oxide, ethylene oxide and gamma-radiation. Toxicology. 2006; 219(1-3): 220-229. https://doi.org/10.1016/j.tox.2005.11.021
- Genotoxicity of ethylene oxide: A review of micronucleus assay results in human population vol.770, 2016, https://doi.org/10.1016/j.mrrev.2016.05.002
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