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Yam Extracts Increase Cell Proliferation and Bone Matrix Protein Collagen Synthesis of Murine Osteoblastic MC3T3-E1 Cells

  • Shin, Mee-Young (Department of Food Science and Nutrition, College of Human Ecology, Andong National University) ;
  • Alcantara, Ethel H. (Department of Food Science and Nutrition, College of Human Ecology, Andong National University) ;
  • Park, Youn-Moon (Department of Food Science and Biotechnology, Andong National University) ;
  • Kwon, Soon-Tae (Department of Horticulture and Breeding, College of Natural Sciences, Andong National University) ;
  • Kwun, In-Sook (Department of Food Science and Nutrition, College of Human Ecology, Andong National University)
  • Received : 2011.12.14
  • Accepted : 2011.12.16
  • Published : 2011.12.31

Abstract

Yam extracts (Dioscorea batatas) have been reported to possess a variety of functions. However, studies on its osteogenic properties are limited. In this study, we investigated the effect of ethanol and water extracts on osteoblast proliferation and bone matrix protein synthesis, type I collagen and alkaline phosphatase (ALP), using osteoblastic MC3T3-E1 cell model. MC3T3-E1 cells were cultured with yam ethanol and water extracts (0~30 mg/L) within 39 days of osteoblast differentiation period. Cell proliferation was measured by MTT assay. Bone matrix proteins were assessed by the accumulation of type I collagen and ALP activity by staining the cell layers for matrix staining. Also, the secreted (media) matrix protein concentration (type I collagen) and enzyme activity (ALP) were measured colorimetrically. Yam ethanol and water extracts stimulated cell proliferation within the range of 15~30 mg/L at 15 day treatment. The accumulation of type I collagen in the extracellular matrix, as well as secreted collagen in the media, increased with increasing doses of yam ethanol (3~15 mg/L) and water (3~30 mg/L) extracts. ALP activity was not affected by yam ethanol extracts. Our results demonstrated that yam extracts stimulated osteoblast proliferation and enhanced the accumulation of the collagenous bone matrix protein type I collagen in the extracellular matrix. These results suggest that yam extracts may be a potential activator for bone formation by increasing osteoblast proliferation and increasing bone matrix protein type I collagen. Before confirming the osteogenic action of yam, further studies for clarifying how and whereby yam extracts can stimulate this ostegenesis action are required.

References

  1. Wang D, Christensen K, Chawla K, Xiao G, Krebsbach PH, Franceschi RT. 1999. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14: 893-903. https://doi.org/10.1359/jbmr.1999.14.6.893
  2. Rodan GA, Martin TJ. 2000. Therapeutic approaches to bone diseases. Science 289: 1508-1514. https://doi.org/10.1126/science.289.5484.1508
  3. Putnam SE, Scutt AM, Bicknell K, Priestley CM, Williamson EM. 2007. Natural products as alternative treatments for metabolic bone disorders and for maintenance of bone health. Phytother Res 21: 99-112. https://doi.org/10.1002/ptr.2030
  4. Bharti AC, Takada Y, Aggarwal BB. 2004. Curcumin (diferuloylmethane) inhibits receptor activator of NF-kappa B ligand-induced NF-kappa B activation in osteoclast precursors and suppresses osteoclastogenesis. J Immunol 172: 5940-5947. https://doi.org/10.4049/jimmunol.172.10.5940
  5. Yin J, Han N, Liu Z, Xu X, Zhang B, Kadota S. 2008. The in vitro anti-osteoporotic activity of some diarylheptanoids and lignans from the rhizomes of Dioscorea spongiosa. Planta Med 74: 1451-1453. https://doi.org/10.1055/s-2008-1081340
  6. Yin J, Han N, Liu Z, Song S, Kadota S. 2010. The in vitro antiosteoporotic activity of some glycosides in Dioscorea spongiosa. Biol Pharm Bull 33: 316-320. https://doi.org/10.1248/bpb.33.316
  7. Yen ML, Su JL, Chien CL, Tseng KW, Yang CY, Chen WF, Chang CC, Kuo ML. 2005. Diosgenin induces hypoxia- inducible factor-1 activation and angiogenesis through estrogen receptor-related phosphatidylinositol 3-kinase/Akt and p38 mitogen-activated protein kinase pathways in osteoblasts. Mol Pharmacol 68: 1061-1073. https://doi.org/10.1124/mol.104.010082
  8. Higdon K, Scott A, Tucci M, Benghuzzi H, Tsao A, Puckett A, Cason Z, Hughes J. 2001. The use of estrogen, DHEA, and diosgenin in a sustained delivery setting as a novel treatment approach for osteoporosis in the ovariectomized adult rat model. Biomed Sci Instrum 37: 281-286.
  9. Alcantara EH, Shin MY, Sohn HY, Park YM, Kim T, Lim JH, Jeong HJ, Kwon ST, Kwun IS. 2011. Diosgenin stimulates osteogenic activity by increasing bone matrix protein synthesis and bone-specific transcription factor Runx2 in osteoblastic MC3T3-E1 cells. J Nutr Biochem 22: 1055-1063. https://doi.org/10.1016/j.jnutbio.2010.09.003
  10. Cho YE, Alcantara E, Kumaran S, Son KH, Sohn HY, Lee JH, Choi CS, Ha TY, Kwun IS. 2010. Red yeast rice stimulates osteoblast proliferation and increases alkaline phosphatase activity in MC3T3-E1 cells. Nutr Res 30: 501-510. https://doi.org/10.1016/j.nutres.2010.06.011
  11. Kim MH, Shim KS, Lee SU, Kim YS, Min YK, Kim SH. 2010. Stimulatory effect of undecylenic acid on mouse osteoblast differentiation. Phytother Res 24: 559-564.
  12. Maeda T, Matsunuma A, Kawane T, Horiuchi N. 2001. Simvastatin promotes osteoblast differentiation and mineralization in MC3T3-E1 cells. Biochem Biophys Res Commun 280: 874-877. https://doi.org/10.1006/bbrc.2000.4232
  13. Shen CL, Yeh JK, Cao JJ, Wang JS. 2009. Green tea and bone metabolism. Nutr Res 29: 437-456. https://doi.org/10.1016/j.nutres.2009.06.008
  14. Sethi G, Aggarwal BB. 2007. Mending the bones with natural products. Chem Biol 14: 738-740. https://doi.org/10.1016/j.chembiol.2007.07.002
  15. Peng KY, Horng LY, Sung HC, Huang HC, Wu RT. 2011. Antiosteoporotic activity of Dioscorea alata L. cv. Phyto through driving mesenchymal stem cells differentiation for bone formation. Evid Based Complement Alternat Med 29: 712892.
  16. Chiang SS, Chang SP, Pan TM. 2011. Osteoprotective effect of Monascus-fermented dioscorea in ovariectomized rat model of postmenopausal osteoporosis. J Agric Food Chem 59: 9150-9157. https://doi.org/10.1021/jf201640j
  17. Xing GS, Lou JS, Wang ZB, Yu SL, Wang Y, Sheng L. 2007. Effects of Dioscorea septemloba on bone metabolism in ovariectomized rats. Zhongguo Zhong Yao Za Zhi 32: 1909-1913.
  18. Hsu KH, Chang CC, Tsai HD, Tsai FJ, Hsieh YY. 2008. Effects of yam and diosgenin on calpain systems in skeletal muscle of ovariectomized rats. Taiwan J Obstet Gynecol 47: 180-186. https://doi.org/10.1016/S1028-4559(08)60077-7
  19. Komesaroff PA, Black CV, Cable V, Sudhir K. 2001. Effects of wild yam extract on menopausal symptoms, lipids and sex hormones in healthy menopausal women. Climacteric 4: 144-150. https://doi.org/10.1080/713605087
  20. Lian JB, Stein GS. 1992. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 3: 269-305. https://doi.org/10.1177/10454411920030030501
  21. Lynch MP, Stein JL, Stein GS, Lian JB. 1995. The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. Exp Cell Res 216: 35-45. https://doi.org/10.1006/excr.1995.1005
  22. Wu X, Itoh N, Taniguchi T, Hirano J, Nakanishi T, Tanaka K. 2004. Stimulation of differentiation in sodium-dependent vitamin C transporter 2 overexpressing MC3T3-E1 osteoblasts. Biochem Biophys Res Commun 317: 1159-1164. https://doi.org/10.1016/j.bbrc.2004.03.158
  23. Balcerzak M, Hamade E, Zhang L, Pikula S, Azzar G, Radisson J, Bandorowicz-Pikula J, Buchet R. 2003. The roles of annexins and alkaline phosphatase in mineralization process. Acta Biochim Pol 50: 1019-1038.
  24. Murshed M, Harmey D, Millan JL, McKee MD, Karsenty G. 2005. Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone. Genes Dev 19: 1093-1104. https://doi.org/10.1101/gad.1276205

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