Alteration of Inflammatory Cytokines by Volatile Organic Compounds in T Lymphocytes

  • Lee, Ji-Sook (Department of Clinical Laboratory Science, Wonkwang Health Science University) ;
  • Kim, In-Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University and Eulji University Medical Sciences Research Center)
  • Received : 2011.03.15
  • Accepted : 2011.03.19
  • Published : 2011.03.31

Abstract

In the present study, we investigated whether volatile organic compounds induce inflammatory response in human T lymphocytes by evaluating the alteration of inflammatory cytokines. Volatile organic compounds such as formaldehyde, o-xylene, benzene, and hydroquinone have no cytotoxic effects on Jurkat T cells at a high concentration of 50 ${\mu}M$ for 48 h. IL-2, IL-4, IL-13, TNF-${\alpha}$ and IFN-${\gamma}$ were increased after the treatment with volatile organic compounds, although alteration of cytokines is different among volatile organic compounds. LPS as a positive control increased the secretion of IL-2, IL-4, IL-13, TNF-${\alpha}$ and IFN-${\gamma}$. MCP-1 and CCL17 (thymus and activation-regulated chemokine, TARC) were weakly increased after the treatment with volatile organic compounds but the amount of the increased cytokine was below 20 pg/ml. These results suggest that the measurement of cytokine in Jurkat T cells may be used as a useful method for evaluating the toxicity of volatile organic compounds in immune response.

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