Effects of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) Treatment on the Monosodium Iodoacetate-induced Early Stage Osteoarthritis in Rats

가감소속명탕이 Monosodium Iodoacetate로 유발된 골관절염의 초기변화에 미치는 영향

  • Park, Dong-Soo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Jeong, Su-Hyeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Soon-Joong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Se-Myung University)
  • 박동수 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 정수현 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실)
  • Received : 2011.09.22
  • Accepted : 2011.10.11
  • Published : 2011.10.30

Abstract

Objectives: This study was performed to investigate the effects of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) on the monosodium iodoacetate(MIA) induced early state osteoarthritis in rats. Methods: Osteoarthritis was induced by injection of MIA(0.25 mg) into knee joints of rats. Osteoarthritis rats were divided into control(n=8) and treated=8 group respectively, Control group was taken distilled water and treated group was taken extracts of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) by orally for 20 days. Body weight was measured at 0, 5, 10, 15, 20 days after MIA injection. At the end of experiment, gross and histopathological examination on the articular cartilages of the knee joints were performed. Proteoglycan(PG) content of articular cartilages were analysed by safranine O staining method. The content of tumor necrosis $factor-{\alpha}(TNF-{\alpha})$, $interleukin-1{\beta}(IL-1{\beta})$ in synovial fluids were analysed by enzyme-inked inmunosorbent assay(ELISA) method. And also cycloxygenase-2(COX-2), matrix metalloproteinase 3(MMP-3), inducible nitric oxide synthase(iNOS), calpain immunochistochemical examination on the knee joints were performed. Results: PG content in articular cartilages of the treated group was significantly increased compared with control group. Histopathological osteoarthritic score of the treated group was significantly decreased compared with control group. $TNF-{\alpha}$ content in synovial fluids, expression of iNOS and calpain in synovial membrane of the treated group were significantly decreased compared with control group. But body weight, $1L-1{\beta}$ content in synovial fluids, expression of iNOS and MMP-3 of the treated group were not significantly changed compared with control group. Conclusions: On the basis of these results, we conclude that Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) has anti-arthritic effects on the MIA induced early stage osteoarthritis in rats.

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