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Effects of Dietary Supplementation of Fermented Garlic Powder and Fluid on Growth Performance, Immune Responses, Blood Components, and Disease Resistance against Edwardsiella tarda and Streptococcus iniae in Olive Flounder Paralichthys olivaceus

사료 내 발효마늘분말과 발효마늘착즙액의 첨가가 넙치(Paralichthys olivaceus)의 성장, 면역반응, 혈액성분 및 어병세균(Edwardsiella tarda 및 Streptococcus iniae)에 대한 질병저항성에 미치는 영향

  • 김강웅 (국립수산과학원 사료연구센터) ;
  • 김성삼 (제주대 해양생명과학과) ;
  • 정준범 (제주대 해양생명과학과) ;
  • 전유진 (제주대 해양생명과학과) ;
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 안철민 (국립수산과학원 사료연구센터) ;
  • 이경준 (제주대 해양생명과학과)
  • Received : 2011.10.26
  • Accepted : 2011.12.07
  • Published : 2011.12.30

Abstract

Two consecutive studies were conducted to evaluate the effects of dietary supplementation with fermented garlic powder (FGP) or fermented garlic fluid (FGF) on growth performance, immune responses, and disease resistance of olive flounder Paralichthys olivaceus. In experiment I, olive flounder (BW: 65 g) were fed four experimental diets formulated to contain 0%, 0.5%, 1%, and 1.5% FGP (designated as FGP-0, FGP-0.5, FGP-1, and FGP-1.5, respectively). After the 10-weeks feeding trial, feed intake was significantly lower in fish fed the FGP-0.5 and FGP-1.0 diets, as compared to those fed the control diet. Fish fed the FGP-0 and FGP-0.5 diets showed significantly lower survival, as compared to the other treatments. Dietary supplementation with FGP resulted in higher non-specific immune responses than the FGP-0 group. Plasma cholesterol and triglyceride levels decreased as dietary FGP level increased. In experiment II, olive flounder (BW: 65 g) were fed four experimental diets for 10 weeks. The diets were prepared with a commercial expanded pellet to have 0%, 0.25%, 0.5%, and 1% FGF (designated as FGF-0, FGF-0.25, FGF-0.5, and FGF-1, respectively) by adsorption. At the end of the second feeding trial, feed intake was significantly lower in fish fed the FGF-0 diet, as compared to other treatments. Fish fed the FGF-0.25 and FGF-0.5 diets exhibited significantly lower cholesterol levels, as compared to other treatments. Lysozyme activity significantly increased with increases in dietary FGF. Cumulative mortality in a challenge test with Streptococcus iniae was significantly lower in the fish groups fed FGF-supplemented diets than in fish fed the control diet. The results of this study indicated that dietary supplementation with FGP or FGF can enhance the non-specific immune responses and disease resistance of olive flounder against S. iniae.

Acknowledgement

Grant : 고효율 배합사료 개발 및 실용화 연구, 친환경 유기양식 생산 매뉴얼 개발을 통한 양식넙치의 고품질화 및 가공기술 개발

Supported by : 국립수산과학원

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