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A Multicellular Spheroid Formation and Extraction Chip Using Removable Cell Trapping Barriers

한시적 세포포집 구조물을 이용한 다세포 스페로이드 형성 및 추출칩

  • Jin, Hye-Jin (Dept. of Bio and Brain Engineering, KAIST) ;
  • Kim, Tae-Yoon (Dept. of Bio and Brain Engineering, KAIST) ;
  • Cho, Young-Ho (Dept. of Bio and Brain Engineering, KAIST) ;
  • Gu, Jin-Mo (Cancer Research Center, Samsung Medical Center) ;
  • Kim, Jhin-Gook (Cancer Research Center, Samsung Medical Center) ;
  • Oh, Yong-Soo (Central R&D Institute, Samsung Electromechanics Co.)
  • 진혜진 (한국과학기술원 바이오및뇌공학과) ;
  • 김태윤 (한국과학기술원 바이오및뇌공학과) ;
  • 조영호 (한국과학기술원 바이오및뇌공학과) ;
  • 구진모 (삼성서울병원 암연구센터) ;
  • 김진국 (삼성서울병원 암연구센터) ;
  • 오용수 (삼성전기 중앙연구소)
  • Received : 2010.04.07
  • Accepted : 2010.11.25
  • Published : 2011.02.01

Abstract

We propose a spheroid chip that uses removable cell trapping barriers and that is capable of forming and extracting multicellular spheroids. By using a conventional well plate and flask, it is difficult to form small-sized spheroids, which resemble avascular 3D cell-cell interaction. It was difficult to extract spheroids using conventional microchips and fixed cell trapping barriers. The proposed chip, however, facilitates both formation and extraction of spheroids by using removable cell trapping barriers formed by membrane deflection. The cell trapping barriers, formed at the membrane pressure of 50 kPa, hold the cells in the trapping region at a cell inlet pressure of 145.155 Pa. After incubation for 24 h, the trapped cells form uniform spheroids. We successfully extract the spheroids at a cell inlet pressure of 5 kPa after removing the membrane pressure. The extracted spheroids have a diameter of $197.2{\pm}11.7Bm$ with a viability of $80.3{\pm}7.7%$. Using the proposed chip, uniform spheroids can be formed and these spheroids can be safely extracted for carrying out the post-processing of spheroids.

Keywords

Multicellular Spheroid;Cell Trapping;Spheroid Formation;Spheroid Extraction

Acknowledgement

Supported by : KAIST 세포벤치연구센터

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