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Biological Activities of Water and Ethanol Extracts from Two Varieties of Rubus coreanus Miquel Fruits

  • Yin, Yu (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
  • Received : 2010.11.01
  • Accepted : 2011.02.10
  • Published : 2011.03.31

Abstract

The potential biological activities of water and ethanol extracts from two varieties of Rubus coreanus Miquel fruits produced in the Gochang-gun (GR) and Hoengseong-gun (HR) regions of Korea were examined. The hydroxyl radical ($\cdot$OH) scavenging activity, reducing power, lipid peroxidation inhibitory activity, and antiproliferative activity on cancer cells by these extracts were examined, and $\alpha$-amylase and $\alpha$-glucosidase inhibition assays were also performed. The EtOH extract from GR showed high hydroxyl radical scavenging activity ($EC_{50}=119.47{\pm}5.13\;{\mu}g/mL$), lipid peroxidation inhibitory activity ($EC_{50}=213.45{\pm}3.14\;{\mu}g/mL$) and a concentration dependence, with OD values ranging from 0.15 to 0.56 (50 to 200 ${\mu}g/mL$), for reducing power. The EtOH extract from GR has the highest antiproliferative activities on MDA-MB-231 and HepG2 cancer cells among four extracts. Meanwhile, all extracts showed certain $\alpha$-amylase and $\alpha$-glucosidase inhibition activities. These results indicate that extracts from two varieties of R. coreanus fruits have significant antioxidant, anti-diabetic and anti-tumorigenic activities, which suggest these extracts could be a potential source for pharmaceutical.

References

  1. Liu JK, Head E, Gharib AM, Yuan WJ, Ingersell RT, Hagen TM, Cotman CW, Ames BN. 2002. Memory loss in old rats is associated with brain mitochondrial decay and RNA/DNA oxidation: Partial reversal by feeding acetyl- L-carnitine and /or R-$\alpha$-lipoic acid. Proc Natl Acad Sci USA 99: 2356-2361. https://doi.org/10.1073/pnas.261709299
  2. Choi JM, Han J, Yoon BS, Chung JH, Shin DB, Lee SK, Hwang JK, Ryang R. 2006. Antioxidant properties of tannic acid and its inhibitory effects on paraquat-induced oxidative stress in mice. Food Sci Biotechnol 15: 728-734.
  3. Athukorala Y, Kim KN, Jeon YJ. 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 44: 1065-1074. https://doi.org/10.1016/j.fct.2006.01.011
  4. Headig D, Tarnow L, Kuhn J, Kleesiek K, Götting C. 2008. Identification of a xylosyltransferase II gene haplotype marker for diabetic nephropathy in type 1 diabetes. Clin Chim Acta 398: 90-94. https://doi.org/10.1016/j.cca.2008.08.019
  5. Jain S, Saraf S. 2008. Type 2 diabetes mellitus-its global prevalence and therapeutic strategies. Diab Met Syndr Clin Res Rev 79: 14-27.
  6. Holman RR, Turner RC, Cull CA. 1999. A randomized double-blind trial of acarbose in type 2 diabetes shows improved glycemic control over 3 years (UK Prospective diabetes study 44). Emerg Treat Tech 22: 960-964.
  7. Ells LJ, Seal CJ, Kettliz B, Bal W, Mathers C. 2005. Postprandial glycaemic, lipaemic and haemostatic responses to ingestion of rapidly and slowly digested starches in healthy young women. Br J Nutr 94: 948-955. https://doi.org/10.1079/BJN20051554
  8. Wallace AJ, Monro JA, Brown RC, Frampton CM. 2008. A glucose reference curve is the optimum method to determine the glycemic glucose equivalent values of foods in humans. Nutr Res 28: 753-759. https://doi.org/10.1016/j.nutres.2008.09.005
  9. Ju HK, Cho EJ, Jang MH, Lee YY, Hong SS, Park JH, Kwon SW. 2009. Characterization of increased phenolic compounds from fermented Bokbunja (Rubus coreanus Miq.) and related antioxidant activity. J Pharm Biomed Anal 49: 820-827. https://doi.org/10.1016/j.jpba.2008.12.024
  10. Park YS, Chang HG. 2003. Latic acid fermentation and biological activities of Rubus coreanus. Korean J Soc Agric Chem Biotechnol 46: 367-375.
  11. Yin Y, Heo SI, Wang MH. 2008. Antioxidant and anticancer activities of methanol and water extracts from leaves of Cirsium japonicum. J Appl Biol Chem 51: 160-164. https://doi.org/10.3839/jabc.2008.030
  12. Masao H, Yang XW, Miyashiro H, Nabma T. 1993. Inhibitory effects of monomeric and dimeric phenylpropanoids from mice on lipid peroxidation in vivo and in vitro. Phytother Res 7: 395-401. https://doi.org/10.1002/ptr.2650070603
  13. Oyaizu M. 1986. Studies on products of browning reaction prepared from glucosamine. Japanese J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  14. Maeda K, Kakabayashi S, Matsubara H. 1985. Complete amino acid sequence of an $\alpha$-amylase inhibitor in wheat kernel (0.19-inhibitor). Biochi Biophys Acta 828: 213-221. https://doi.org/10.1016/0167-4838(85)90299-7
  15. Kim YM, Jeong YK. 2005. Inhibitory effect of pine extract on $\alpha$-glucosidase activity and postprandial hyperglycemia. Nutrition 21: 756-761. https://doi.org/10.1016/j.nut.2004.10.014
  16. Popiolkiewicz J, Polkowski K, Skierski JS, Mazurek AP. 2005. In vitro toxicity evaluation in the development of new anticancer drugs-genistein glycosides. Cancer Lett 229: 67-75. https://doi.org/10.1016/j.canlet.2005.01.014
  17. Ding T, Rahman SME, Purev U, Oh DH. 2010. Modeling of Escherichia coli O157:H7 growth at various storage temperatures on beef treated with electrolyzed oxidizing water. J Food Process Eng 97: 497-503. https://doi.org/10.1016/j.jfoodeng.2009.11.007
  18. Siriwardhana SSKW, Shahidi F. 2002. Antiradical activity of extracts of almond and its by-products. JAOSC 79: 903-908. https://doi.org/10.1007/s11746-002-0577-4
  19. Amin A, Yazdanparast R. 2007. Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food Chem 104: 21-29. https://doi.org/10.1016/j.foodchem.2006.10.066
  20. Prasad NK, Divakar S, Shivamurthy GR, Aradhya SM. 2005. Isolation of a free radical scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). J Sci Food Agric 85: 1461-1468. https://doi.org/10.1002/jsfa.2125
  21. Duh PD. 1998. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free radical and active oxygen. JAOCS 75: 455-461. https://doi.org/10.1007/s11746-998-0248-8
  22. Gordon MH. 1990. The mechanism of antioxidant action in vitro. In Food Antioxidants. Hudson BJF, ed. Elsevier, Applied Science, London, UK. p 1-18.
  23. Puls W, Keup U, Krause HP, Thomas G, Hoffmeister F. 1977. Glucosidase inhibition. A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia. Naturwissenschaften 64: 536-537. https://doi.org/10.1007/BF00483562
  24. Lee CL, Yang XT, Wan JMF. 2006. The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor. Enzyme Microb Technol 38: 14-21. https://doi.org/10.1016/j.enzmictec.2004.10.009
  25. Yin Y, Heo SI, Roh KS, Wang MH. 2009. Biological activities of fractions from methanolic extract of Picrasma quassioides. J Plant Biol 52: 325-331. https://doi.org/10.1007/s12374-009-9042-x

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