Antioxidative Activity of a Medicinal Herb Mixture Prepared through the Traditional Antidiabetic Prescription

당뇨처방에 근거한 생약재 복합물의 항산화 활성

  • Lee, Gee-Dong (Major of Nutrition Education, Graduate School of Education, Joongbu University)
  • 이기동 (중부대학교 교육대학원 영양교육전공)
  • Received : 2011.06.08
  • Accepted : 2011.10.28
  • Published : 2011.12.30


The antioxidative activity of a medicinal herb mixture combined with traditional natural herbal materials was investigated. The medicinal herb mixture yielded 35.00% water extracts and 25.33% 80% ethanol extracts. The ethyl acetate fraction yields were 0.64% in the water extracts and 3.76% in the 80% ethanol extracts. The total flavonoid contents of the water and 80% ethanol extracts were 2.34 and 2.42%, respectively, and their total phenolic contents were 5.04 and 4.56%. The total flavonoid and phenolic contents of the ethyl acetate fraction were the highest in the various solvent extracts. The extracts were rich in salicylic and ${\rho}$-coumaric acids. The electron-donating ability of the medicinal herb mixture was 43.32% in the water extracts and 41.32% in the 80% ethanol extracts, and the nitrite-scavenging ability was 9.68% in the water extracts and 8.94% in the 80% ethanol extracts.


Supported by : 지식경제부


  1. Kim JG, Kang YM, Eum GS, Ko YM, Kim TY (2003) Antioxidative activity and antimicrobial activity of extracts from medicinal plants. J Agr Life Sci, 37, 69-75
  2. Pari L, Umamaheswari J (2000) Antihyperglycemic activity of Musa sapientum flowers: effect on lipid peroxidation in alloxan diabetic rats. Phytother Res, 14, 1-3<1::AID-PTR580>3.0.CO;2-O
  3. Matsui T, Yoshimoto C, Osajima K, Oki T, Osajima Y, Oki T, Osajima Y (1996) In vitro survey of $\alpha$-glucosidase inhibitory food components. Biosci Biotechnol Biochem, 60, 2019-2022
  4. Bansky D, Barolet R (1990) Chinese Herbal Medicine Formulas and Strategies. Eastland Press, Seattle, USA, p 7-8
  5. Goleberg I (1994) Functional Foods. Champman & Hall Press, New York, USA, p 350-550
  6. Sadaki O (1996) The development of functional foods and material. Bio-industry, 13, 44-50
  7. Bansky D, Barolet R (1990) Chinese Herbal Medicine Formulas and Strategies. Eastland Press, Seattle, p 7-8
  8. Korea Food & Drug Administration (2009) Accessed May. 25
  9. Lee YJ, Won DH (1982) Pharmacognostical studies on Polygonatum sibiricum Redoute. Bull Sk Pharma Sci, 31, 185-198
  10. Kim JK, Lee YJ (1980) Pharmacognostical studies on the rhizome of Polygonatum robustum Nakai. Kor J Pharmacog, 11, 69-74
  11. Byun SH (1995) Immunohistochemical study for the effects of Yukmizihwangtang and Yukmizihwangtang- Deer Antler on the diabetic rats. The Journal of Jeahan Oriental Medical Academy, 1, 1-16
  12. Lee YG, Kim SD (2000) The antidiabetic effect of Gamiyookmijihwangtang on diabetes-prone BB rats. Korean J Food Sci Technol, 32, 1206-1212
  13. Choi HY, Jung TY, Seo BI, Kim JD, Park DI (2003) The effect of several herbal medicines which appear frequently as the effects of hypoglycemia in streptozotocininduced diabetic rats. Kor J Herbology, 18, 73-78
  14. Kim JS, Na CS (2004) Effect of Rehmanniae Radix and pear phenolic compound on the STZ-treated mice for induction of diabetes. J Korean Soc Food Sci Nutr, 33, 66-71
  15. Kim JS (2004) Effect of Rhemanniae Radix on the hyperglycemic mice induced with streptozotocin. J Korean Soc Food Sci Nutr, 33, 1133-1138
  16. Kim HC (2001) Herbal Pharmacology. Jipmoondang, Seoul, Korea, p 92
  17. Kang KA, Chae S, Koh YS, Kim JS, Lee JH, You HJ, Hyun JW (2005) Protective effect of Puerariae Radix on oxidative stress induced by hydrogen peroxide and streptozotocin-induced diabetic rats. Plata Med, 70, 113-116
  18. Chen WC, Hayakawa S, Yamamoto T, Su HC, Liu IM, Cheng JT (2004) Mediation of beta-endorphin by the isoflavone puerarin to lower plasma glucose in streptozotocin-induced diabetic rats. Planta Medica, 70, 113-116
  19. Cho YJ, Ju IS, Kim BC, Lee WS, Kim MJ, Lee BG, An BJ, Kim JH, Kwon OJ (2007) Biological activity of Omija (Schizandra chinensis Baillon) extracts. J Korean Soc Appl Biol Chem, 50, 198-203
  20. Kim JO, Lee GD, Kim JJ, Kim JH, Kim KS (2007) Antidiabetic effects of Schizantra chinensis Baillon water extract in streptozotocin-induced insulin-dependent diabetic rats. Lab Anim Res, 23, 5-8
  21. Ko BS, Park SK, Choi SB, Jun DW, Choi MK, Park SM (2004) A study of hypoglycemic effects of crude extracts of Schizandrae Fructus. J Korean Soc Appl Bio Chem, 47, 258-264
  22. Chizuko H, Kanji Y, Toshihide Y (2004) Efficacy of Bofu-tsusho-san, an oriental herbal medicine, in obese Japanese women with impaired glucose tolerance. Clin Exp Pharmacol Physiol, 31, 614-619
  23. Aida K, Tawata M, Shindo H, Onaya T, Sasaki T, Yamaguchi T, Clin T, Mitsuhashi H (1990) Isoliquiritigenin: A new aldose reductase inhibitor from Glycyrrhizae radix. Planta Med, 56, 254-258
  24. Lee GD (2006) Development of microencapsulated new materials for blood glucose and blood pressure control. Final Report of Ministry of Knowledge, 10018069
  25. Heo J (1994) Donguibogam. Haeseongsa, Seoul, Korea
  26. The Korean Pharmaceutical Association & Herb Medicine Policy Committee (2001) (WeonSaeg) Hantagdogam. Academy Press, Seoul, Korea
  27. Shin MK (1997) Clinical Traditional Herbalogy (5th ed). Younglimsa, Seoul, Korea
  28. Amerinem MA, Ough CS (1958) Method for Analysis of Musts and Win. Wiley & Sons, New York, USA, p 176-180
  29. Lee JM, Son ES, Oh SS, Han DS (2001) Contents of total flavonoid and biological activities of edible plants. Korean J Dietary Culture, 16, 504-514
  30. Liang Y, Ma W, Lu J, Wu Y (2001) Comparison of chemical compositions of Ilex latifolia Thumb and Camellia sinensis L. Food Chem, 75, 339-343
  31. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200
  32. Kato H, Lee IE, Chyuen NV, Kim SB, Hayase F (1987) Inhibitory of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem, 51, 1333-1338
  33. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH (1987) Degradation of carcinogenic nitrosamine formation factor by natural food components-1. Nitrite-scavenging effects of vegetable extracts. Bull Korean Fish Soc, 20, 463-468
  34. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS (2000) Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower. J Korean Soc Food Sci Nutr, 29, 1127-1132
  35. Whang HJ, Han WS, Yoon KR (2001) Quantitative analysis of total phenolic content in apple. Anal Sci Technol, 14, 377-383
  36. Yokozawa T, Nakagawa T, Kitani K (2002) Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem, 50, 3549-3552
  37. Kwon TD, Ryu SP, Jang EC, Lee SC (2002) Effect of green tea polyphenol ingestion on blood lipid, MDA and SOD in rats. Korean J Exercise Nutr, 6, 85-88
  38. Choi HJ, Park JH, Han HS, Son JH, Son GM, Bae JH, Choi C (2004) Effect of polyphenol compound from Korean pear on lipid metabolism. J Korean Soc Food Sci Nutr, 33, 299-304
  39. Hyun SH, Jung SK, Jwa MK, Song CK, Kim JH, Lim SB (2007) Screening of antioxidants and cosmeceuticals from natural plant resources in Jeju Island. Korean J Food Sci Technol, 39, 200-208
  40. Choi YM, Kim MH, Shin JJ, Park JM, Lee JS (2003) The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr, 32, 723-727
  41. Choi JH, Oh SK (1985) Studies on the anti-aging action of Korean Ginseng. Korean J Food Sci Technol, 17, 506-515
  42. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J Food Sci Technol, 37, 233-240
  43. Chung HJ (1999) Antioxidative effect of ethanolic extracts of some tea materials on red pepper seed oil. J Korean Soc Food Sci Nutr, 28, 1316-1320
  44. Ju IO, Jung GT, Ryu J, Choi JS, Choi YG (2005) Chemical components and physiological activities of bamboo (Phyllostachys bambusoides Starf) extracts prepared with different methods. Korean J Food Sci Technol, 37, 542-548
  45. Min SH, Lee BR (2007) Antioxidant activity of medicinal plant extracts cultivated in Jecheon. Korean J Food Culture, 22, 336-341
  46. Hotchkiss JH (1998) A review of current literature on N-nitroso compounds in foods. Adv Food Res, 31, 54-115
  47. Macrae R, Robinson RK, Sadler MJ (1993) Encyclopedia of Food Science Food Technology and Nutrition. Academic press, New York, USA, p 3240-3249

Cited by

  1. Enhancement of Anti-wrinkle Activities of Abeliophyllum distichum Nakai through Low Temperature Extraction Process vol.23, pp.3, 2015,