Hypoglycemic Effects of a Medicinal Herb Mixture Prepared through the Traditional Antidiabetic Prescription

당뇨 처방에 근거한 생약재 복합물의 혈당강하 효과

  • Kim, Jung-Ok (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • Lee, Gee-Dong (Major of Nutrition Education, Graduate School of Education, Joongbu University)
  • 김정옥 (대구경북한방산업진흥원) ;
  • 이기동 (중부대학교 교육대학원 영양교육전공)
  • Received : 2011.07.07
  • Accepted : 2011.12.09
  • Published : 2011.12.30


For the purpose of investigating the in vitro antidiabetic activity of a medicinal herb mixture prepared through traditional antidiabetic prescription, the study analyzed the existence of insulin-similar components and examined ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibition activity. As a result of arranging the medicinal herb mixture extracts over the 3T3-L1 fibroblast in the concentration of $10{\mu}g/mL$, which confirmed that it included much of insulin sensitizer components as 151.7% in the differentiation of 3T3-L1 fibroblast. The inhibition activity against ${\alpha}$-amylase of the medicinal herb mixture extracts as hypoglycemic agent were 38.4, 31.5 and 16.6% in the concentration of 10.0, 1.0 and 0.1 mg/mL, respectively. The inhibition activity against ${\alpha}$-glucosidase of the medicinal herb mixture extracts were 81.3, 35.8 and 26.7% in the concentration of 10.0, 1.0 and 0.1 mg/mL, respectively. The inhibition activity against ${\alpha}$-glucosidase in the ethyl acetate fractions of the water and 80% ethanol extracts were 66.9% and 55.1%, respectively, the highest levels in the various solvent extracts.


Supported by : 지식경제부


  1. Kim TW, Kwon YB, Lee JH, Yang IS, Youm JK, Lee HS, Moon JY (1996) A study on the antidiabetic effect of mulberry fruits. Korean J Seric Sci, 38, 100-107
  2. Torsdottir I, Alpsten M, Andersson H, Einasson S (1989) Dietary guar gum effects on postprandial blood glucose, insulin and hydroxyproline in humans. J Nutr, 119, 1925-1931
  3. Blackburn NA, Redfern JS, Jaris H, Holgate AM, Hanning I, Scarpello JHB, Johnson IT, Read NW (1984) The mechanism of action of guar gum in improving glucose tolerance in man. Clin Sci, 66, 329-336
  4. Puls HP, Krause L, Muller H, Schutt R, Thomas G (1984) Inhibitors of the rate of carbohydrate and lipid absorption by the intestine. Int J Obesity, 8, 181-190
  5. Lee YR, Nam SH, Kang MY (2006) Hypoglycemic effect of the giant embryonic rice supplementation on streptozotocin-induced diabetic rats. Korean J Food Sci Technol, 38, 427-431
  6. Kim YY, Cho RW, Chung SH, Koo SJ (1999) Anti-hyperglycemic effect of Cortex Mori radicis in db/db mice. Korean J Food Sci Technol, 31, 1057-1064
  7. Ortiz-Andrade RR, Garcia-Jimenez S, Castillo-Espana P, Ramirez-Avila G, Villalobos-Molina R, Estrada-Soto S (2007) $\alpha$-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: an antihyperglycemic agent. J Ethnopharmacol, 109, 48-53
  8. Heo J (1994) Donguibogam. Haeseongsa, Seoul, Korea
  9. Cho YJ, Ju IS, Kim BC, Lee WS, Kim MJ, Lee BG, An BJ, Kim JH, Kwon OJ (2007) Biological activity of Omija (Schizandra chinensis Baillon) extracts. J Korean Soc Appl Biol Chem, 50, 198-203
  10. Kim JO, Lee GD, Kim JJ, Kim JH, Kim KS (2007) Antidiabetic effects of Schizantra chinensis Baillon water extract in streptozotocin-induced insulin-dependent diabetic rats. Lab Anim Res, 23, 5-8
  11. Ko BS, Park SK, Choi SB, Jun DW, Choi MK, Park SM (2004) A study of hypoglycemic effects of crude extracts of Schizandrae Fructus. J Korean Soc Appl Bio Chem, 47, 258-264
  12. Byun SH (1995) Immunohistochemical study for the effects of Yukmizihwangtang and Yukmizihwangtang-Deer Antler on the diabetic rats. The Journal of Jeahan Oriental Medical Academy, 1, 1-16
  13. Lee YG, Kim SD (2000) The antidiabetic effect of Gamiyookmijihwangtang on diabetes-prone BB rats. Korean J Food Sci Technol, 32, 1206-1212
  14. Choi HY, Jung TY, Seo BI, Kim JD, Park DI (2003) The effect of several herbal medicines which appear frequently as the effects of hypoglycemia in streptozotocininduced diabetic rats. Kor J Herbology, 18, 73-78
  15. Kim JS, Na CS (2004) Effect of Rehmanniae Radix and pear phenolic compound on the STZ-treated mice for induction of diabetes. J Korean Soc Food Sci Nutr, 33, 66-71
  16. Kim JS (2004) Effect of Rhemanniae Radix on the hyperglycemic mice induced with streptozotocin. J Korean Soc Food Sci Nutr, 33, 1133-1138
  17. Kim HC (2001) Herbal Pharmacology. Jipmoondang, Seoul, Korea, p 92
  18. Kang KA, Chae S, Koh YS, Kim JS, Lee JH, You HJ, Hyun JW (2005) Protective effect of Puerariae Radix on oxidative stress induced by hydrogen peroxide and streptozotocin-induced diabetic rats. Plata Med, 70, 113-116
  19. Chen WC, Hayakawa S, Yamamoto T, Su HC, Liu IM, Cheng JT (2004) Mediation of beta-endorphin by the isoflavone puerarin to lower plasma glucose in streptozotocin-induced diabetic rats. Planta Medica, 70, 113-116
  20. Lee YJ, Won DH (1982) Pharmacognostical studies on Polygonatum sibiricum Redoute. Bull Sk Pharma Sci, 31, 185-198
  21. Kim JK, Lee YJ (1980) Pharmacognostical studies on the rhizome of Polygonatum robustum Nakai. Kor J Pharmacog, 11, 69-74
  22. Chizuko H, Kanji Y, Toshihide Y (2004) Efficacy of Bofu-tsusho-san, an oriental herbal medicine, in obese Japanese women with impaired glucose tolerance. Clin Exp Pharmacol Physiol, 31, 614-619
  23. Aida K, Tawata M, Shindo H, Onaya T, Sasaki T, Yamaguchi T, Clin T, Mitsuhashi H (1990) Isoliquiritigenin: A new aldose reductase inhibitor from Glycyrrhizae radix. Planta Med, 56, 254-258
  24. The Korean Pharmaceutical Association & Herb Medicine Policy Committee (2001) (WeonSaeg) HanYagDoGam. Academy Press, Seoul, Korea
  25. Shin MK (1997) Clinical Traditional Herbalogy (5th ed). Younglimsa, Seoul, Korea
  26. Lee GD (2006) Development of microencapsulated new materials for blood glucose and blood pressure control. Final Report of Ministry of Knowledge, 10018069
  27. Lee OH, Lee HB, Son JY (2004) Antimicrobial activities and nitrite-scavenging ability of olive leaf fractions. Korean J Soc Food Cookery Sci, 20, 204-210
  28. Kameda K, Chikaki M, Morimoto C, Jiang M, Okuda H (1996) Insulin like action of trans-10-hydroxy-2-decanoic acid and its related substance. J Traditional Med, 13, 456-457
  29. Dennis JE, Caplan AI (1996) Differentiation potential of conditionally immortalized mesenchymal progenitor cells from adult marrow of H-2Kb-tsA58 transgenic mouse. J Cell Physiol, 167, 523-538<523::AID-JCP16>3.0.CO;2-4
  30. Hansawasdi C, Kawabata J, Kasai T (2000) $\alpha$-Amylase inhibitor from Roselle (Hibiscus sabdariffa Linn.) tea. Biosci Biotechnol Biochem, 64, 1041-1043
  31. Angelov A, Putyrski M, Liebl W (2006) Molecular and biochemical characterization of alpha-glucosidase and alpha-mannosidase and their clustered genes from the themoacidophilic archaeon Picrophilus torridus. J Bacteriol, 188, 7123-7131
  32. Huo H, Guo X, Hong S, Jiang M, Liu X, Liao K (2003) Lipid rafts/caveolae are essential for insulin-like growth factor-1 receptor signaling during 3T3-L1 preadipocyte differentiation induction. J Biol Chem, 278, 11561- 11569
  33. Sekiya K, Zheng Y (1997) Effect of medical plant on preadipocyte differentiation. J Traditional Med, 14, 356-357
  34. Broadhust CL, Polansky MM, Anderson RA (2000) Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem, 48, 849-852
  35. Ju YS, Ko BS (2002) Screening of insulin-like substances from traditional herbs if diabetes prescription in Donguibogam. J Korean Soc Agric Chem Biotechnol, 45, 47-52
  36. Ko BS, Kim HK, Park SM (2003) Insulin sensitizing and insulin-like effects of water extracts from Kalopanax pictus Nakai in 3T3-L1 adipocyte. J Korean Soc Agric Chem Biotechnol, 45, 42-46
  37. Defronzo RA (1981) The effect of insulin on renal sodium metabolism. Diabetologia, 21, 165-171
  38. Steiner G, Haynes F, Yoshino G (1984) Hyperinsulinemia and in vivo very-low-density lipoprotein triglyceride kinetics. Am J Physio, 246, 187-192
  39. Young IR, Stout RW (1987) Effects of insulin and glucose on the cells of the arterial wall: Interaction of insulin with dibutyryl cyclic AMP and low density lipoprotein in arterial cells. Diabetes Metab, 13, 301-306
  40. Yu HJ, Song OG (1985) Dietary therapy for diabetes mellitus. Diabetes Mellitus, 9, 21-25
  41. Heo GB (1985) Exercise therapy for diabetes mellitus. Diabetes Mellitus, 9, 5-10
  42. Koivisto VA (1993) Insulin therapy in type II diabetes. Diabetes Care, 16, 29-39
  43. Jenkins DJ, Wolever TM, Jenkins AL (1988) Starchy foods and glycemic index. Diabetes Care, 11, 149-159
  44. Haller H (1998) The clinical importance of postprandial glucose. Diabetes Res Clin Pract, 40, S43-S49
  45. Lebovitz HE (1998) Postprandial hyperglycemic state: importance and consequences. Diabetes Res Clin Pract, 40, S27-S28
  46. Bailey CJ (1999) Insulin resistance and antidiabetic drugs. Biochem Phaumacol, 58, 1511-1520
  47. Zhang BB, Moller DE (2000) New approaches in the treatment of type 2 diabetes. Curr Opin Chem Biol, 4, 461-467
  48. Tattersall R (1993) $\alpha$-glucosidase inhibition as an adjunct to the treatment of type I diabetes mellitus. Diabet Med, 10, 658-662
  49. Hanefeld M (1998) The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J Diabetes Complications, 12, 228-237
  50. Xu ML, Wang L, Xu GF, Wang MH (2011) Antidiabetes and angiotensin converting enzyme inhibitory activity of Sonchusasper (L) Hillextract. Kor J Pharmacogn, 42, 61-67
  51. Cha MR, Park JH, Choi YH, Choi CW, Hong KS, Choi SU, Kim YS, Kim YK, Kim YH, Ryu SY (2009) Alpha-glucosidase inhibitors from the branches extract of Cotinus coggygria. Kor J Pharmacogn, 40, 229-232

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