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Antimutagenicity and Immuno Activity of Extracts from Epimedium koreanum Nakai Containing Different Icariin Quantity

Icariin 함량에 따른 삼지구엽초 추출물의 항돌연변이 및 면역활성

  • Park, Myoung-Su (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seo-Jin (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Forghani, Fereidoun (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Rahman, S.M.E. (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Eo, Ji-Hyun (Dept. of Food Science and Technology and Functional Food Research Center Chonnam National University) ;
  • Eun, Jong-Bang (Dept. of Food Science and Technology and Functional Food Research Center Chonnam National University) ;
  • Oh, Deog-Hwan (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University)
  • 박명수 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 김서진 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 페레이둔 포가니 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 라만 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 어지현 (전남대학교 식품공학.기능성식품연구센터) ;
  • 은종방 (전남대학교 식품공학.기능성식품연구센터) ;
  • 오덕환 (강원대학교 바이오산업공학부 식품생명공학과)
  • Received : 2011.06.29
  • Accepted : 2011.12.02
  • Published : 2011.12.30

Abstract

Epimedium koreanum Nakai is a wild herb commonly consumed in South Korea due to its beneficial health effects. In the present study, the antimutagenic and immunoactivities of extracts from E. koreanum Nakai containing different icariin quantities were investigated for food use. In the Ames test, both the water and ethanol extracts were found not to have a mutagenic effect on Salmonella typhimurium TA98 and TA100, respectively. The E. koreanum Nakai extracts showed over 80 and 90% antimutagenic effects on benzo(${\alpha}$)pyrene (B(a)P) in S. typhimurium TA98 and TA100, respectively. Moreover, all the extracts showed over 70% antimutagenicity on S. typhimurium TA98 and TA100 against 4-nitroquinoline-1-oxide (4NQO). The E. koreanum Nakai extract with ethanol showed strong antimutagenic activity, higher than that of the water extract and the sequenced KE9412, KE9408, and KE9405. In the immunomodulating activity test, the effect of E. koreanum Nakai on the B (Rhamos) and T (Jukat) cells were investigated. The immunoactivity results showed that the growth and viability of the B and T cells increased and were activated more in KE9405 (1.8 times), KE9408 (1.6 times), and KE9412 (1.32 times) in the water extracts, and least in KE9412 (1.74 times), KE9408 (1.52 times), and KE9405 (1.4 times) in the ethanol extracts. In the case of both the water and ethanol extracts ($500{\mu}g/mL$) from E. koreanum Nakai, the highest cell number of the human B (Rhamos) and T (Jukat) cells was observed on day 4 in KE9405 and KE9412, and on day 5 in KE9408. Based on the obtained results, the development of E. koreanum Nakai as a food material is recommended.

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