Deacidification Effect of Campbell Early Must through Carbonic-Maceration Treatment: Isolation and Properties of the Bacteria Associated with Deacidification

Carbonic Maceration처리에 의한 Campbell Early 발효액의 감산 효과: 감산 관련 미생물의 분리 및 특성

  • Chang, Eun-Ha (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jeong, Seok-Tae (Fermentation & Food Processing Division, National Academy of Agricultural Science, RDA) ;
  • Jeong, Sung-Min (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lim, Byung-Sun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Noh, Jung-Ho (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Kyo-Sun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Seo-Jun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Jong-Uck (Department of Food Science & Technology, Kyungpook National University)
  • Received : 2011.05.17
  • Accepted : 2011.11.18
  • Published : 2011.12.30


The grape cultivar Campbell Early has high levels of malic acid as well as tartaric acid. The high concentration of total acid in the Campbell Early wine is a critical aspect of the wine's sensory characteristics. To prevent the deterioration of the wine's quality, which is caused by the strong sour taste derived from the raw material in wine making, the deacidification factor was investigated via carbonic maceration under different temperature conditions, especially in the presence or absence of malolactic bacteria. Based on the results of the presence test of the malolactic bacteria during carbonic-maceration treatment, Lactobacillus brevis, Lactobacillus plantarum, and Streptococcus thermophilus were characterized morphologically and were identified via biochemical tests and 16S-rRNA-gene-sequencing analysis. The isolated strains were found not to consume malic acid and to produce lactic acid. Moreover, these strains were consumed as soluble solids. The isolated strains are popularly known as lactic-acid bacteria and should have produced lactic acid from glucose. The Oenococcus oeni of the malolactic bacteria was not isolated. These results showed that the isolated strains are not deacidified during carbonic-maceration treatment.


  1. Beelman RB, Gallander JF (1979) Wine deacidification. Adv Food Res, 25, 1-53
  2. Ruffner HP (1982) Metabolism of tartaric and malic acids in Vitis: a review, Part A. Vitis, 21, 247-259
  3. Park WM, Park HG, Rhee SJ, Lee CH, Yoon KE (2002) Suitability of domestic grape, cultivar Campbell Early, for production of red wine. Korean J Food Sci Technol, 34, 590-596
  4. DeBolt S, Cook DR, Ford CM (2006) L-tartaric acid synthesis from vitamin C in higher plants. Proc Natl Acad Sci, USA 103, 5608-5613
  5. Graham HF (1993) Wine microbiology and biotechnology. Harwood Academic Publishers, Chur, Switzland
  6. Gao C, Fleet GH (1995) Degradation of malic and tartaric acids by high density cell suspensions of wine yeasts. Food Microbiol, 12, 65-71
  7. Fonseca A (1992) Utilization of tartaric acid and related compounds by yeasts: taxonomic implications. Canadian J Microbiol, 38, 1242-1251
  8. Boulton RB, Singleton VL, Bisson LF, Kunkee RE (1996) Principles and practices of winemaking. Aspen Publishers, Gaithersburg, Maryland, USA p.320-351
  9. Fonseca A, Fell JW, Kurtzman CP, Spencer-Martins I (2000) Candida tartarivorans sp. nov., an anamorphic ascomycetous yeast with the capacity to degrade L- and meso-tartaric acid. Int J Syst Evol Microbiol, 50, 389-394
  10. Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek, 73, 331-371
  11. Chang EH, Jeong ST, Roh JH, Jeong SM, Park SJ, Lee HC, Choi JU (2010) Enological characteristics of campbell early grape must studied using various carbonic maceration temperatures. Korean J Food Preserv, 17, 881-888
  12. Spranger MI, Climaco MC, Sun B, Eiriz N, Fortunato C, Nunes A, Leandro MC, Avelar ML, Belchior AP (2004) Differentiation of red winemaking technologies by phenolic and volatile composition. Analytica Chimica Acta, 513, 151-161
  13. Lee JK, Kim JS (2006) Study on the deacidification of wine made from Campbell Early. Korean J Food Sci Technol, 38, 408-413
  14. Chang EH, Jeong ST, Roh JH, Yun HK, Park KS, Choi JU (2008) Effect on wine quality of pre-treatment of grapes prior to alcohol fermentation. Korean J Food Preserv, 15, 824-831
  15. Lee SO, Pack MY (1980) Malo-lactic bacteria in korean winery environment and their potential use in wine making. Korean J Appl Microbiol Bioeng, 8, 193-198
  16. Kim JH, Kim JI (1999) Identification and fermentation characteristics of lactic acid bacteria isolated from dongchimi as starter for radish juice. Korean J Microbiol, 35, 307-314
  17. Park MH, Lyu DK, Ryu CH (2002) Characteristics of high acidity producing acetic acid bacteria isolated from industrial vinegar fermentation. J Korean Soc Food Sci Nutr, 31, 394-398
  18. Caspritz G, Radler F (1983) Malolactic enzyme of Lactobacillus plantarum. J Biologi Chemi, 258, 4907-4910
  19. Hernandez H, Estrella I, Perez-Gordo M, Alegria EG, Tenorio C, Ruiz-Larrea F, Moreno-Arribas MV (2007) Contribution of malolactic fermentation by Oenococcus oeni and Lactobacillus plantarum to the changes in the nonanthocyanin polyphenolic composition of red wine. J Agric Food Chem, 55, 5260-5266
  20. Pozo-Bayon MA, Alegria EC, Polo MC, Tenorio C, Martin-Alvarez PJ, Calvo de la Banda MT, Ruiz-Larrea F, Moreno-Arribas MV (2005) Wine volatile and amino acid composition after malolactic fermentation: Effect of Oenococcus oeni and Lactobacilluis plantarum starter cultures. J Agric Food Chem, 53, 8729-8735
  21. Yoo JY, Seon HM, Shin DH, Min BY (1984) Enological characteristics of korean grape and quality evaluation of their wine. Korean J Appl Microbiol Bioeng, 12, 185-190
  22. Kim JS, Kim SH, Han JS (1999) Effects of sugar and yeast addition on red wine fermentation using campbell early. Korean J Food Sci Technol, 31, 516-521

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