Effects of Natural Substances and Polyphenols on Candida albicans, Neisseria gonorrhoeae and L. acidofillus in the Reproductive Organ

생식기의 Candida albicans, Neisseria gonorrhoeae 및 Lactobacillus acidofillus에 대한 천연물질과 Polyphenol의 효과

  • 한상진 (한림대학교 자연과학대학 생명과학과)
  • Received : 2011.11.17
  • Accepted : 2011.12.05
  • Published : 2011.12.31

Abstract

Antimicrobial studies on reproductive organs have been scarcely reported. Candida albicans and Neisseria gonorrhoea test to find out the natural substances of various concentrations in the antimicrobial experiments have been made. Antimicrobial effect of biopole as the natural compound biosynthesis matter that contain anti-inflammatory material, catechin polyphenols and lactic acid as a single natural substance on C. albicans were of great significance. Also the results of antimicrobial effects of biopole, catechin and lactic acid as a single natural substance on N. gonorrhoea, respectively, showed lower concentration than those of the antimicrobial effects on C. albicans. Through the survival of Lactobacillus acidofillus that acts for the protection of the genital tissue the importance of lactic acid was confirmed. Lactobacillus acidofillus protection and C. albicans firmly into disinfected to low concentrations of the natural mixture from biopole and catechin with lactic acid were found and the antimicrobial effects of this natural mixture on N. gonorrhoea were perfect. C. albicans and N. gonorrhoea to disinfect the optimal natural mixture from 2% concentration biopole, 0.2% concentration of catechin and 2% lactic acid were obtained. Through the survival of Lactobacillus acidofillus in the reproductive organs protectable effects were acquired to prevent the infections of reproductive tissue and the recurrence.

Acknowledgement

Supported by : 한림대학교

References

  1. 보건복지부 (2011) 65세 이상 노인 성병환자 연평균 12.7% 증가, 성병환자 현황.
  2. Workowski, K. A. and Levine, W. C. (2002) Sexually transmitted diseases treatment guidelines, Centers for Disease Control and Prevention. MMWR Recomm. Rep. 51(RR-6): 1- 78.
  3. Martins, H. P., da Silva, M. C., Cássia, L., Svidzinski, T. I. and Consolaro, M. E. (2011) Efficacy of fluconazole and nystatin in the treatment of vaginal Candida species, Vet. Immunol. Immunopathol. 9: 45-54.
  4. Issa, S. Y., Badran, E. F., Aqel, K. F. and Shehabi, A. A. (2011) Epidemiological characteristics of Candida species colonizing oral and rectal sites of Jordanian infants. BMC Pediatr. 11: 1471-1479.
  5. Norata, G. D., Marchesi, P., Passamonti, S., Pirollo, A., Violi, F. and Catapano, A. I. (2007) Anti-inflammatory and antiatherogenic effects of catechin, caffeic acid and trans-resveratrol in apolipoprotein deficient mice. Atherosclerosis 191(2): 265-271. https://doi.org/10.1016/j.atherosclerosis.2006.05.047
  6. Moore, R. J., Jackson, K. G. and Minihane, A. M. (2009) Green tea(Camella simensis) catechins and vascular function. Br. J. Nutr. 15: 1-13.
  7. Fenton, K. A., Ison, C., Johnson, A. P., Rudd, E., Soltani, M. and Martin, I. (2003) Ciprofloxacin resistance in Neisseria gonorrhoeae in England and Wales in 2002. Lancet. 361: 1867-1869. https://doi.org/10.1016/S0140-6736(03)13489-7
  8. Valcic, S., Muders, F., Jacobson Waschington, L. and Timmermann, B. N. (1999) Antioxicantic chemistry. Res. Toxicol. 12(4): 382-386. https://doi.org/10.1021/tx990003t
  9. Lippincott, W. and Amp, W. (2008) Antimicrobial effects of epigallocatechin-3-gallate (EGCG) against clinical isolates. J. Burn Care and Res. 29(2): 21.
  10. Yokoyama, M., Nogucchi, M., Nakao, Y., Ysunaga, M., Yamasaki, F. and Iwasaka, T. (2008) Antioriliferative effects of the major tea polyphenol, (-)-epigallocatechingallate and retinoic acid in cervical adenocarcinoma. Gynecol Oncol. 108(2): 326-331. https://doi.org/10.1016/j.ygyno.2007.10.013
  11. Wächtler, B., Wilson, D. and Hube, B. (2011) Candida albicans adhesion to and invasion and damage of vaginal epithelial cells: stage-specific inhibition by clotrimazole and bifonazole. Antimicrob. Agents Chemother. 55(9): 4436- 4439. https://doi.org/10.1128/AAC.00144-11
  12. Zhang, Q., Tang, X., Lu, Q., Zhang, Z., Rao, J. and Le, Ad. (2008) Green tea extract and (-)-epigallocatechingallate inhibit hypoxia-and serum-induced HIF-1alpha protein accumulation and VEGF expression in human cervical carcinoma at hepatina cells. Mol. cancer Ther. 5(5): 1227-1238.
  13. Choi, J. M., Han, J., Woon, BS., Chung, J. H., Shin, D. B. and Lee, S. K. (2006) Antioxidant properties of tannic acid and its inhibitory effects on paraquat-induced oxidative stress in mice. Food Sci. Biotechnol. 15(5): 728-734.
  14. Choi, S. I., Hong, Y., Lee, J. H. and Kim, G. H. (2008) Antioxidant and antimicrobial activities of the ethanol extract of Allium victoriallis L. var. platyphyllum. Food Sci. Biotechnol. 17(2): 313-318 .
  15. Rice, E. C., Miller, N. J. and Paraganda G. (1996) Stuructureantioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20: 933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  16. Manach, C., Morand, C., Crespy, V., Demigne, C., Texier, O., Regerat, F. and Remesy, C. (1988) Quercetin is recovered in human plasma as conjugated derivatives which retain antioxidant properties. FEBS Lett. 426: 331-336.
  17. Kim, Y. S. and Han, S. S. (1999) Antiherpetic activities of natural quercetin alone and in combinations with nucleoside antiherpstic agents. Kor. J. Pharmacogn. 30(2): 151-157.
  18. Kim, Y. G. (1995) Antivacteria activities of korean and foreign green tea extract. Kor. J. Env. Hlth. Soc. 21: 39-49.
  19. Williams, T. and Amp, W. (2008) Antimicrobial effects of flavonoids with pigallocatechin-3-gallate(EGCG) against clinical bacteria. J. burn care and Res. 29(2): 21.
  20. Cibley, L. J. (2010) Vulvovagivitis: Current approach to diagnosis and treatment. The female patients 42: 45-48, 56.
  21. Epstein, J. B., Emerton, S., Le, N. D. and Stevenson-Moore, P. (1996) A double-blind crossover trial of Oral Balance gel and Biotene toothpaste versus placebo in patients with xerostomia following radiation therapy. Oral Oncol. 35(2): 132- 137.
  22. Nakachi, K., Suga, K. and Imai, K. (1995) Preventive Effects of Drinking catechin on Cardiovascular Disease and Cancer, Kor. J. Foodscience 28(4): 11-16.
  23. Yokozawa, T., Cho, E. J., Hara, Y. and Kitani, K. (2000) Antioxidative activity of green tea treated with radical initiator 2, 2'-Azobis(2-amidinopropane) dihydrochloride, J. Agric Food Chem. 48(10): 5068-5073. https://doi.org/10.1021/jf000253b
  24. Moran, G. P, Coleman, D. C. and Sullivan, D. J. (2011) Candida albicans versus Candida dubliniensis: Why is C. albicans more pathogenic? Int. J. Microbiol. 18: 2012-2021.
  25. Jebashree, H. S., Kingsley, S. J., Sathish, E. S. and Devapriya, D. (2011) Antimicrobial activity of few medicinal plants against clinically isolated human cariogenic pathogens-an in vitro study. ISRN Dent. 2011(2011): 1-6.
  26. Cho, J. and Lee, D. G. (2011) Oxidative stress by antimicrobial peptide pleurocidin triggers apoptosis in Candida albicans. Biochemie 93(10): 1873-1879. https://doi.org/10.1016/j.biochi.2011.07.011
  27. Brahmachari, G., Mandal, N. C., Jash, S. K., Roy, R., Mandal, L. C., Mukhopadhyay, A., Behera, B., Majhi, S., Mondal, A. and Gangopadhyay, A. (2011) Evaluation of the antimicrobial potential of two flavonoids isolated from limnophila plants. Chem. Biodivers. 8(6): 1139-1151. https://doi.org/10.1002/cbdv.201000264
  28. Han, S. Z. (2009) Antimicrobial effects of polyphenol in green tea on Candida albicans and Nesseria gonorrhoeae, J. Kor. Tea Soc. 15(3): 77-83.
  29. Jang, S. Y., Ryu, S. Y. and Kim, S. D. (2003) Antifungal activity of plant extracts aginst Pityrosporum ovale and Candida albicans, Kor. J. Pharmacogn. 34(4): 303-307.
  30. Yoo, S. C. and Suh, J. S. (1974) Antifungal actions of crude drug water extracts on Candida albicans, Kor. J. Pharmacog. 5(3): 147-154.
  31. Arruda, A .L., Vieira, C. J., Sousa, D. G., Oliveira, R. F. and Castilho, R. O. (2011) Jacaranda cuspidifolia Mart. (Bignoniaceae) as antibacterial agent. Med. Food 8(3): 332-339.