- Volume 37 Issue 4
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Effect of Nigella sativa Oil on Melanogenesis
니겔라 사티바 오일의 미백 효능에 관한 연구
- Lee, Su-Yeon (R&D Center, Bioland) ;
- Lee, Sae-Mi (R&D Center, Bioland) ;
- Heo, Woo-Beom (R&D Center, Bioland) ;
- Kim, Jin-Guk (R&D Center, Bioland) ;
- Kim, Young-Heui (R&D Center, Bioland)
- 이수연 ((주)바이오랜드 생명공학연구소) ;
- 이새미 ((주)바이오랜드 생명공학연구소) ;
- 허우범 ((주)바이오랜드 생명공학연구소) ;
- 김진국 ((주)바이오랜드 생명공학연구소) ;
- 김영희 ((주)바이오랜드 생명공학연구소)
- Received : 2011.09.15
- Accepted : 2011.09.22
- Published : 2011.12.30
In order to investigate the potential of Nigella sativa (N. sativa) oil as an active ingredient for whitening cosmetics, we prepared N. sativa oil. We measured its inhibitory effects on mushroom tyrosinase activity, cellular tyrosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. N. sativa oil and its components showed inhibitory activity against mushroom tyrosinase and melanin synthesis. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it reduced melanin production up to 86 % at a concentration of 10 mg/mL without cytotoxicity. In the study on the melanogenic protein expressions by using RT-PCR and Western blot, N. sativa oil and its components inhibited expression of tyrosinase protein, which is a well-known key protein on melanogenesis, and tyrosinase expression was gradually decreased in a dose-dependent manner. Therefore, this result suggests that N. sativa oil could be used as an active ingredient for whitening cosmetics.
- G. J. Fisher, H. S, Talwar, J. Lin, and J. J Voorhees, Molecular mechanism of photoaging in human skin in vivo and their prevention by all-trans retinoic acid, Blackwell Publishing Ltd., (1999).
- H. Z. hill, W. Li, P. Xin, and D. L. Michell, Melanin : a two edged sword, Pigment Cell Res., 10, 158 (1997). https://doi.org/10.1111/j.1600-0749.1997.tb00478.x
- B. W. Choi, B. H. Lee, K. J. Kang, E. S. Lee, and N. H. Lee, Screening of the tyrosinase inhibitors from marine algae and medicinal plants, Kor. J. pharmacogn., 29(3), 237 (1998).
- V. J. Hearing and M. Jimenez, Mammalian tyrosinase-the critical regulatory control point in melanocyte pigment., Int. J. Biochem., 19, 1141 (1987). https://doi.org/10.1016/0020-711X(87)90095-4
- H. M. Aoki, Identifiacation of a new microphthalmia(Mi)-interacting protein, rKr2, involved in the regulation of melanogenesis, IFSCC (1998).
- M. Seibers, C. Paine, E. Sharlow, P. Andrade Gordon, M. costanzo, M. Eisinger, and S. S. Shapiro, The protease-activated receptor 2 regulates pigmentation via keratinocyte-melanocyte interation, Experimental Cell Res., 254, 25 (2000). https://doi.org/10.1006/excr.1999.4692
T. Motogawa, Inhibition of
$\alpha$-MSH induced melanogenesis by Sophorae radix extracts, Frangrance J., 9, 38 (2000).
- G. Negroiu, N. Branza-Nichita, A. J. Petrescu, R. A. Dwek, and S. M. Petrescu, Protein specific N-glycosylation of tyrosinase and tyrosinase-related protein-1 in B16 mouse melanoma cells, Biochem. J., 344, 659 (1999). https://doi.org/10.1042/0264-6021:3440659
- D. R. Worthen, O. A. Ghosheh, and P. A. Crooks, The in vitro anti-tumor activity of some crude and purified components of blackseed, L. Anticancer Res., 18(3A), 1527 (1998).
- B. H. Alil and G. Blunden, Pharmacological and toxicological properties of Nigella sativa, Phytotherapy Res., 17(4), 299 (2003). https://doi.org/10.1002/ptr.1309
- T. M. El-Hadiyah, M. Raza, O. Y. Mohammed, and A. A. Abdallah, Evaluation of Nigella sativa seed constituents for their in vivo toxicity in mice., Natural Products Sci., 9(1), 22 (2003).
- Y. Asakawa, R. Matsuda, M. Tori, and M. Sono, Efficient preparetion of some biologically active substances from natural and nonnatural aromatic compounds by m-chloroperbenzoic acid oxidation, J. Org., Chem., 53(23), 5453 (1988). https://doi.org/10.1021/jo00258a010