Effect of Nigella sativa Oil on Melanogenesis

니겔라 사티바 오일의 미백 효능에 관한 연구

  • 이수연 ((주)바이오랜드 생명공학연구소) ;
  • 이새미 ((주)바이오랜드 생명공학연구소) ;
  • 허우범 ((주)바이오랜드 생명공학연구소) ;
  • 김진국 ((주)바이오랜드 생명공학연구소) ;
  • 김영희 ((주)바이오랜드 생명공학연구소)
  • Received : 2011.09.15
  • Accepted : 2011.09.22
  • Published : 2011.12.30


In order to investigate the potential of Nigella sativa (N. sativa) oil as an active ingredient for whitening cosmetics, we prepared N. sativa oil. We measured its inhibitory effects on mushroom tyrosinase activity, cellular tyrosinase activity, and melanin synthesis inhibitory activity in B16 melanoma cells. N. sativa oil and its components showed inhibitory activity against mushroom tyrosinase and melanin synthesis. In a melanin synthesis inhibition assay using mouse B16-F10 melanoma cell, it reduced melanin production up to 86 % at a concentration of 10 mg/mL without cytotoxicity. In the study on the melanogenic protein expressions by using RT-PCR and Western blot, N. sativa oil and its components inhibited expression of tyrosinase protein, which is a well-known key protein on melanogenesis, and tyrosinase expression was gradually decreased in a dose-dependent manner. Therefore, this result suggests that N. sativa oil could be used as an active ingredient for whitening cosmetics.


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