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Assessment of Korean Paddy Soil Microbial Community Structure by Use of Quantitative Real-time PCR Assays

한국의 논 토양 미생물 다양성 분석을 위한 Quantitative Real-time PCR의 응용

  • Choe, Myeong-Eun (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University)
  • 최명은 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이인중 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 신재호 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2011.11.30
  • Accepted : 2011.12.12
  • Published : 2011.12.31

Abstract

BACKGROUND: In order to develop effective assessment method for Korean paddy soil microbial community structure, reliable genomic DNA extraction method from paddy soil and quantitative real-time PCR (qRT-PCR) method are needed to establish METHODS AND RESULTS: Out of six conventional soil genomic DNA extraction methods, anion exchange resin purification method was turn to be the most reliable. Various PCR primers for distinguishing five bacterial phylum (${\alpha}$-Proteobacteria, ${\beta}$-Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes), all bacteria, and all fungi were tested. Various qRT-PCR temperature conditions were also tested by repeating experiment. Finally, both genomic DNA extraction and qRT-PCR methods for paddy soil were well established. CONCLUSION: Quantitative real-time PCR (qRT-PCR) method to assess paddy soil microbial community was established.

Acknowledgement

Grant : Cooperative Research Program for Agricultural Science & Technology Development

Supported by : Rural Development Administration

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