Antibacterial Effects of Extracts of Thuja Orientalis cv Aurea Nana Cones against Food-spoilage and Food-borne Pathogens



Yang, Xiao Nan;Hwang, Cher-Won;Kwon, Gi-Seok;Kang, Sun-Chul

  • 투고 : 2011.10.18
  • 심사 : 2011.12.24
  • 발행 : 2011.12.31


BACKGROUND: Nowadays, Chemical antiseptics have become great problems for health and environmental, so that developing of new substitutes for chemical antiseptics is more and more important. Natural product is a kind of environment-friendly additive that could be used as antiseptic in food industry. Thuja orientalis cv Aurea Nana is a gymnospermous plant of the family Cupressaceae, native to northwestern China and widely naturalised elsewhere in Korea and Japan. This study was aimed to investigate the antibacterial potential of various organic extracts from T. orientalis cones against some food-borne and food-spoilage bacteria. METHODS AND RESULTS: Hexane extract (HE), chloroform extract (CE), ethyl acetate extract (EAE) and methanol extract (ME) were obtained from female cones of T. orientalis. The antibacterial activities of various extracts were tested by standard agar diffusion and minimum inhibitory concentrations (MICs) against five gram-positive and six gram-negative bacteria. Cell viability and morphology change of L. monocytogenes ATCC 10943 treated with hexane extract were also observed. The various extracts displayed remarkable antibacterial effects against all the gram-positive bacteria but did not show any effect against the gram-negative bacteria. Hexane extract has the highest inhibitory effect on cell viability of L. monocytogenes ATCC 10943. SEM observation also demonstrated the damaging effect of the hexane extract on the morphology of L. monocytogenes ATCC 10943 at the minimum inhibitory concentration. CONCLUSION(s): The tested gram-positive bacteria were significantly inhibited by organic extracts of T. orientalis cone. Hexane extract was the most potent against Listeria monocytogenes ATCC 10943, as evidenced by the lowest MIC level and the complete inhibition of cell viability within shortest exposure time, along with SEM observation.


Antibacterial activity;Food-borne and Food-spoilage pathogens;Thuja orientalis cv Aurea Nana


  1. Asili, J., Lambert, M., Ziegler, H.L., Staerk, D., Sairafianpour, M., Witt, M., Asghari, G., Ibrahimi, I.S., and Jaroszewski, J.W., 2004. Labdanes and isopimaranes from Platycladus orientalis and their effects on erythrocyte membrane and on Plasmodium falciparum growth in the erythrocyte host cells,. J. Nat. Prod . 67, 631-637.
  2. Chandrasekaran, M., and Venkatesalu, V., 2004. Antibacterial and antifungal activity of Syzygium jambolanum seeds,. J. Ethnopharmacol. 91, 105-108.
  3. Deba, F., Xuan, T.D., Yasuda, M., and Tawata, S., 2008. Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. var. Radiata., Food Control. 19, 346-352.
  4. Jones, F.A., 1996. Herbs-useful plants. Their role in history and today,. Eur. J. Gastroenterol. Hepatol. 8, 1227-1231.
  5. Guleria, S., Kumar, A., and Tiku, A.K., 2008. Chemical composition and fungitoxic activity of essential oil of Thuja orientalis L. grown in the north-western Himalaya. Z. Naturforsch. , 63c, 211-214.
  6. Hammer, K.A., Carsonl, C.F., and Riley, T.V., 1999. Antimicrobial activity of essential oils and other plant extracts,. J. Appl. Microbiol. 86, 985-990.
  7. Hassanzadeh, M.K., Rahiimizadeh, M., Fazly Bazzaz, B.S., Emami, S.A., and Assili, J., 2001. Chemical and antimicrobial studies of Platycladus orientalis essential oils,. Pharm. Biol. 39, 388-390.
  8. Helander, I.M., Alakomi, H.L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E.J., Gorris, L.G.M., and Wright, A.von., 1998. Characterization of the action of selected essential oil components on gram-negative bacteria,. J. Agric. Food Chem. 46, 3590-3595.
  9. Hoffman, D.L., 1987. The Herb User's Guide. Wellingb., UK: Thorsons Publishing Group.
  10. Kim, J.Y., Kim, H.J., Kim, S.M., Park, K.R., Jang, H.J., Lee, E.H., Jung, S.H., and Ahn, K.S., 2010. Methylene chloride fraction of the leaves of Thuja orientalis inhibits in vitro inflammatory biomarkers by blocking NF-κB and p38 MAPK signaling and protects mice from lethal endotoxemia,. J. Ethnopharmacol. 133, 687-695.
  11. Kockro, R.A., Hampl, J.A., Jansen, B., Peters, G., Scheihing, M., and Giacomelli, R, 2000. Use of scanning electron microscophy to investigate the prophylactic efficacy of rifampin-impregnated CSF shunt catheters,. J. Med. Microbiol. 49, 441-450.
  12. Lawless, J., 1995. The Illustrated Encyclopedia of Essential Oils. Shaftesbury, UK: Element Books Ltd.
  13. Lu, Y.H., Liu, Z.Y., Wang, Z.T., and Wei, D.Z., 2006. Quality evaluation of Platycladus orientalis (L.) Franco through simultaneous determination of four bioactive flavonoids by high-performance liquid chromatography,. J. Pharm. Biomed. Anal. 41, 1186-1190.
  14. Murray, P.P., Baron, E.J., Pfaller, M.A., Tenove, F.C., and Yolke, R.H., 1995. Manual of Clinical Microbiology, ASM, Washington.
  15. Nishiyama, N., Chu, P.J., and Saito, H., 1995. Beneficial effects of biota, a traditional Chinese herbal medicine on learning impairment induced by basal forebrain-lesion in mice,. Biol. Pharm. Bull. 18, 1513-1517.
  16. Nychas, G.J.E., 1995. Natural Antimicrobials from Plants, G.W. Gould, Editor, New methods of food preservation, Blackie Academic Professional, London.
  17. Sikkema, J., De Bont, J.A.M., and Poolman, B., 1994. Interactions of cyclic hydrocarbons with biological membranes,. J. Biol. Chem. 269 (11), 8022-8028.
  18. Singh, A., and Singh, V.K., 2009. Molluscicidal activity of Saraca asoca and Thuja orientalis against the fresh water snail Lymnaea acuminate ., Vet. Parasitol. 164, 206-210.
  19. Vaara, M., 1992. Agents that increase the permeability of the outer membrane,. Microbiol. Rev. 56(3), 395-441.
  20. WHO, 2002. Food Safety and Foodborne Illness. World Health Organization Fact sheet 237, Geneva.
  21. Zhu, J.X., Wang, Y., Kong, L.D., Yang, C., and Zhang, X., 2004. Effects of Biota orientalis extract and its flavonoid constituents, quercetin and rutin on serum uric acid levels in oxonate-induced mice and xanthine dehydrogenase and xanthine oxidase activities in mouse liver,. J. Ethnopharmacol. 93, 133-140.


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