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Isolation and Characterization of Surfactin-producing Bacillus amyloliquefaciens YJ07 from Spent Mushroom (Pleurotus eryngii) Substrates

새송이버섯 수확 후 배지로부터 surfactin 생성 Bacillus amyloliquefaciens YJ07의 분리 및 특성

  • Shin, Pyung Gyun (Division of Mushroom Research, International Institute of Horticultural & Herhal Science, RDA) ;
  • Yoo, Young Bok (Division of Mushroom Research, International Institute of Horticultural & Herhal Science, RDA) ;
  • Cho, Yong Un (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 신평균 (농촌진흥청 인삼특작부 버섯과) ;
  • 유영복 (농촌진흥청 인삼특작부 버섯과) ;
  • 조용운 (경남과학기술대학교 제약공학과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Received : 2011.11.27
  • Accepted : 2011.12.11
  • Published : 2011.12.31

Abstract

Spent mushroom substrates (SMS) is a by-product remaining after a crop of mushrooms. About four surfactin-producing strains were isolated from SMS (Pleurotus eryngii). Among of them, one isolate, which designated to YJ07, potentially showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium. The biochemical characteristics of the strain YJ07 was similar with Bacillus subtilis and Bacillus amyloliquefaciens by Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis of the strain YJ07 also showed that the strain YJ07 was most closely related to Bacillus amyloliquefaciens with sequence similarity of 99.5%. On the basis of their biochemical characteristics and phylogenetic distinctiveness, the strain YJ07 was classified within the genus Bacillus as Bacillus amyloliquefaciens YJ07. The antifungal compound from B. amyloliquefaciens YJ07 was similar to lipopeptide surfactin from Bacillus subtilis by TLC and HPLC analysis.

Keywords

Spent mushroom substrates;Antifungal activity;Surfactin;Bacillus amyloliquefaciens YJ07

Acknowledgement

Supported by : 농촌진흥청