Effects of Yukmigeehwang-hwan Extracts on the Elastase Activity and DPPH and NO Scavenging Activities Original Articles

육미지황환(六味地黃丸)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향

  • Received : 2011.02.18
  • Accepted : 2011.02.28
  • Published : 2011.03.30


Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Yukmigeehwang-hwan extracts (YHE) on elastase activity and anti-oxidative effects of YHE. Methods : The inhibitory effects on elastase and DPPH and NO free radical scavenging activities of YHE were measured. Results : The elastase activity was significantly inhibited by YHE. YHE significantly scavenged DPPH and NO free radicals as well. Conclusion : YHE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that YHE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension.


  1. The Bangje-hak textbook committee. Bangjehak. Seoul : Young-Lim Press. 1999 : 298-300.
  2. Bensky D, Barolet R. Chinese Herbal Medicine Formulas & Strategies. Washington : Eastland Press. 1990 : 263-6.
  3. Bieth JG. The elastases. J Soc Biol. 2001 ; 195(2) : 173-9.
  4. Loukanov T, Geiger R, Agrawal R. Animal models related to congenital heart disease and clinical research in pulmonary hypertension. Cardiology. 2010 ; 116(1) : 18-25.
  5. Dewachter L, Dewachter C, Naeije R. New therapies for pulmonary arterial hypertension: an update on current bench to bedside translation. Expert Opin Investig Drugs. 2010 ; 19(4) : 469-88.
  6. Stenmark KR, Rabinovitch M. Emerging therapies for the treatment of pulmonary hypertension. Pediatr Crit Care Med. 2010 ; 11(2 Suppl) : S85-90.
  7. Huang JB, Liu YL, Sun PW, Lv XD, Bo K, Fan XM. Novel strategy for treatment of pulmonary arterial hypertension: enhancement of apoptosis. Lung. 2010 ; 188(3) : 179-89.
  8. Groneberg DA, Chung KF. Models of chronic obstructive pulmonary disease. Respir Res. 2004 ; 5 : 18.
  9. Mandl I, Darnule TV, Fierer JA, Keller S, Turino GM. Elastin degradation in human and experimental emphysema. Adv Exp Med Biol. 1977 ; 79 : 221-31.
  10. Dubaybo BA, Crowell LA, Thet LA. Changes in tissue fibronectin in elastase induced lung injury. Cell Biol Int Rep. 1991 ; 15(8) : 675-86.
  11. Lucey EC, Keane J, Kuang PP, Snider GL, Goldstein RH. Severity of elastase-induced emphysema is decreased in tumor necrosis factor-alpha and interleukin-1beta receptor-deficient mice. Lab Invest. 2002 ; 82(1) : 79-85.
  12. Hayes JA, Korthy A, Snider GL. The pathology of elastase-induced panacinar emphysema in hamsters. J Pathol. 1975 ; 117(1) : 1-14.
  13. Fischer B, Voynow J. Neutrophil elastase induces MUC5AC messenger RNA expression by an oxidant-dependent mechanism. Chest. 2000 ; 117(5 Suppl 1) : 317S-20S.
  14. Fischer BM, Voynow JA. Neutrophil elastase induces MUC5AC gene expression in airway epithelium via a pathway involving reactive oxygen species. Am J Respir Cell Mol Biol. 2002 ; 26(4) : 447-52.
  15. Kawai M, Harada N, Takeyama H, Okajima K. Neutrophil elastase contributes to the development of ischemia/reperfusion-induced liver injury by decreasing the production of insulinlike growth factor-I in rats. Transl Res. 2010 ; 155(6) : 294-304.
  16. Kraunsoe JA, Claridge TDW, Lowe, G. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry. 1996 ; 35 : 9090-6.
  17. Kim YH, Chung CB, Kim JG, Ko KI, Park SH, Kim JH, Eom SY, Kim YS, Hwang YI, Kim KH. Anti-Wrinkle Activity of Ziyuglycoside I Isolated from a Sanguisorba officinalis Root Extract and Its Application as a Cosmeceutical Ingredient. Biosci Biotechnol Biochem. 2008 ; 72(2) : 303-11.
  18. Shimada K, Fugikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxiation of soy gean oil in cyclodextrin emulsion. J Agric Food Chem. 1992 ; 40 : 945-8.
  19. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. Inhibition of nitrosamine formation by non-dialyzable melanoidins. Agric Biol Chem. 1987 ; 51(5) : 1333-8.
  20. Yang ZY, Guon GX, Lin ZF. Elastolytic activity from Flavobacterium odoratum. Microbial screening and cultivation, enzyme production and purification. Process Biochem. 1994 ; 29 : 427-36.
  21. Wiltried M, Klaus N, Lin ZF. Elastic fibre arrangement in the skin of the pig. Arch Derm Res. 1994 ; 270 : 390-401.
  22. Wiedow O, Schroder JM, Christophers E. An elastase-specific inhibitor of human skin. J Biol Chem. 1990 ; 265 : 14791-5.
  23. Lungarella G, Cavarra E, Lucattelli M, Martorana PA. The dual role of neutrophil elastase in lung destruction and repair. Int J Biochem Cell Biol. 2008 ; 40(6-7) : 1287-96.
  24. Reboud-Ravaux M. Elastase inhibitors. J Soc Biol. 2001 ; 195(2) : 143-50.
  25. Boyer L, Plantier L, Dagouassat M, Lanone S, Goven D, Caramelle P, Berrehar F, Kerbrat S, Dinh-Xuan AT, Crestani B, Le Gouvello S, Boczkowski J. Role of nitric oxide synthases in elastase-induced emphysema. Lab Invest. 2011 ; 91(3) : 353-62.