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Inhibitory Effect of Extracts of Platycodon grandiflorum (the Ballon Flower) on Oxidation and Nitric Oxide Production

도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과

  • Received : 2010.08.10
  • Accepted : 2010.12.17
  • Published : 2011.02.28

Abstract

We explored the effect of extracts of dried Platycodon grandiflorum on production of reactive oxygen species (ROS), glutathione (GSH) and nitric oxide (NO). To determine antioxidant activity in the presence of $H_2O_2$-induced oxidative stress, DCFH-DA (dichlorodihydrofluorescin diacetate) assay was employed. Acetone/methylene chloride (A+M) and methanolic (MeOH) extracts of P. grandiflorum reduced intracellular ROS levels. Of the various tested fractions, n-BuOH fraction showed the highest protective effect in terms of lipid peroxide production. Total GSH levels were measured after treatment of HT1080 cells with the A+M and MeOH extracts, and other solvent fractions, at various concentration. The A+M extacts and 85% (v/v) aqueous MeOH fraction significantly increased GSH levels (p<0.05). When lipopolysaccharide (LPS)-induced NO production was evaluated, all tested crude extracts, and fractions thereof, significantly reduced NO production (p<0.05), and the n-BuOH and 85% (v/v) aqueous MeOH fractions (at 0.05 mg/mL) showed the strongest inhibitory effects. The results showed that the n-BuOH fraction inhibited both cellular oxidation and NO production, and this fraction may thus contain valuable active compounds.

Keywords

platycodon grandiflorum;antioxidant;reactive oxygen species;glutathione;nitric oxide

References

  1. Lim KH (1971) A medicinal Phytology (The details). Dong Myoung Sa, Seoul, p 281
  2. Tada A, Kaneiwa Y, Shoji J, Shibata S (1975) Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D. Chem Pharm Bull, 23, 2965-2972 https://doi.org/10.1248/cpb.23.2965
  3. Akiyama T, Tanaka O, Shibata S (1972) Chemical studies on the oriental plant drugs. XXX. sapogenins of the roots of Platycodon grandiflorum A. De Candolle. (1). Isolation of the sapogenins and the stereochemistry of polygalacic acid. Chem Pharm Bull, 20, 1945-1951 https://doi.org/10.1248/cpb.20.1945
  4. Hong MW (1975) Statistical analyses of Platycodi radix prescriptions. Korean J Pharmacog, 19, 177-188
  5. Kim KS, Osamu E, Shinji I, Hiroshige I (1995) Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia. J Nutr Sci Vitaminol, 41, 485-491 https://doi.org/10.3177/jnsv.41.485
  6. Lee EB (1975) Pharmacological activities of crude platycodin. J Pharm Soc Korean, 19, 164-176
  7. Lee JY, Hwang WI, Lim ST (2004) Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol, 93, 409-415 https://doi.org/10.1016/j.jep.2004.04.017
  8. Lee SI (1981) Chinese parmaceutics. Soo Seo Won, Seoul, p 129
  9. Kim YS, Lee BE, Kim KJ, Lee YT, Cho KB, Chung YC (1998) Antitumor and immunomodulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji, 42, 382-387
  10. Shon MY, Seo JK, Kim HJ, Sung NJ (2001) Chemical compositions and physiological activities of Doraji (Platycodon grandiflorum). J Korean Soc Food Sci Nutr, 30, 717-720
  11. Kim KK, Sung BY, Jung HS, Choi SY, Moon SB (2000) A study on the thermal characteristics of the large low temperature vacuum dryer for biological drying. J Korean Soc Marine Engineers, 24, 427-434
  12. Biesalski HK (2002) Free radical theory of aging. Curr Opin Clin Nutr, 5, 5-10 https://doi.org/10.1097/00075197-200201000-00002
  13. Yu BP (1996) Aging and oxidative stress: Modulation by dietary restriction. Free Radical Bio Med, 21, 651-668 https://doi.org/10.1016/0891-5849(96)00162-1
  14. Block G, Langseth L (1994) Antioxidant vitamins and disease prevention. Food Technol, 48, 80-84
  15. Hatano T (1995) Constituents of natural medicines with scavenging effects on active oxiygen species-tannins and related polyphenols. Nat Med 49, 357-363
  16. Pryored WA (1984) Free radicals in biology. Academic Press. VI., p 371
  17. Nathan CF, Hibbs HB Jr (1991) Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr Opinion Immunol, 3, 65-70 https://doi.org/10.1016/0952-7915(91)90079-G
  18. Lee BH, Baik DS, Yun SU, Shin JM, Kim JH, Yun SY, Kim BH, Kim SB, Shin J E, Song IH (2007) Peripheral nitric oxide activity in patients with Liver cirrhosis. The Korean Journal of Medicine, 73, 251-257
  19. Zhang J, Dawson Nl., Dwson TM, Snyder SH (1994) Nitric oxide activation of poly (ADP-ribose) synthetase in Neurotoxicity. Sci, 263, 687-689 https://doi.org/10.1126/science.8080500
  20. Nguyen T, Brunson D, Crespi CL, Penman BW, Wishnok JS, Tannenbaum SR (1992) DNA damage and mutation in human cells exposed to nitric oxide in vitro. Proc Natl Acad Sci USA, 89, 3030-3034 https://doi.org/10.1073/pnas.89.7.3030
  21. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA (1990) Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA. 87, 1620-1624 https://doi.org/10.1073/pnas.87.4.1620
  22. LeBel CP, Ischiropoulos H, Bondy SC (1992) Evaluation of the probe 2', 7'-Dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem Res Toxicol, 5, 227-231 https://doi.org/10.1021/tx00026a012
  23. Poot M, Verkerk A, Koster JF, Jongkind JF (1986) De novo synthesis of glutathione in human fibroblasts during in vitro ageing and in some metabolic diseases as measured by a flow cytometric method. Biochem Biophys Acta, 883, 580-584 https://doi.org/10.1016/0304-4165(86)90300-4
  24. Green LC, Wagner DA, Logowski GJ, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Anal l Biochem, 126, 131-138 https://doi.org/10.1016/0003-2697(82)90118-X
  25. Lee KJ, Hwang SJ, Choi JH, Jeong HG (2008) Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF-$\kappa$B activation via ROS signal pathway. Cancer Letters, 268, 233-243 https://doi.org/10.1016/j.canlet.2008.03.058
  26. Jeong CH, Shim KH (2006) Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. J Korean Soc Food Sci Nutr, 35, 511-515 https://doi.org/10.3746/jkfn.2006.35.5.511
  27. Kang BY, Kim M, Bae SJ (2002) Enhancement of antioxidation effect of Platycodon grandiflorum with vitamin C on the DLPC liposomes. J Korean Soc Food Sci Nutr, 31, 506-510 https://doi.org/10.3746/jkfn.2002.31.3.506
  28. Wang C, Levis GBS, Lee EB, Levis WR, Lee DW, Kim BS, Park SY, Park E (2004) Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-$\alpha$ in activated RAW 264.7 cells. Int Immunopharmacol, 4, 1039-1049 https://doi.org/10.1016/j.intimp.2004.04.005
  29. Kim SH, Choi JJ, Oh, HT, Chung MJ, Cui CB, Ham SS (2008) Cytoprotective effect by antioxidant activity of Codonopsis lanceolata and Platycodon grandiflorum ethyl acetate fraction in human HepG2 cells. Korean J Food Sci Technol, 40, 696-701

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