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Proteomic Characteristics of Calcium Enriched King Oyster Mushroom (Pleurotus eryngii)

칼슘함량이 강화된 새송이 버섯의 프로테옴 분석

  • Bae, Hee-Sun (Pohang Center for Evaluation of Biomaterials) ;
  • Kim, Dae-Hyun (School of Nano-Bioscience & Chemical Engineering, UNIST) ;
  • Choi, Ung-Kyu (School of Nano-Bioscience & Chemical Engineering, UNIST)
  • 배희선 ((재)포항테크노파크 바이오정보지원센터) ;
  • 김대현 (울산과학기술대학교 나노생명화학공학부) ;
  • 최웅규 (울산과학기술대학교 나노생명화학공학부)
  • Received : 2010.09.02
  • Accepted : 2010.12.02
  • Published : 2011.02.28

Abstract

This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.

Keywords

Ca-enriched king oyster mushroom;proteomic analysis;MALDI-TOF

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