Proteomic Characteristics of Calcium Enriched King Oyster Mushroom (Pleurotus eryngii)

칼슘함량이 강화된 새송이 버섯의 프로테옴 분석

  • Bae, Hee-Sun (Pohang Center for Evaluation of Biomaterials) ;
  • Kim, Dae-Hyun (School of Nano-Bioscience & Chemical Engineering, UNIST) ;
  • Choi, Ung-Kyu (School of Nano-Bioscience & Chemical Engineering, UNIST)
  • 배희선 ((재)포항테크노파크 바이오정보지원센터) ;
  • 김대현 (울산과학기술대학교 나노생명화학공학부) ;
  • 최웅규 (울산과학기술대학교 나노생명화학공학부)
  • Received : 2010.09.02
  • Accepted : 2010.12.02
  • Published : 2011.02.28


This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.


  1. Rajarathnam S, Bano Z. Pleurotus mushrooms. Part 1 A. Morphology, life cycle, taxonomy, breeding, and cultivation. CRC Crit. Rev. Food Sci. 26: 157-223 (1987)
  2. Jin SK, Kim IS, Kim Dh, Jeong KJ, Moon SS. Effect of Pleurotus eryngii and meat particle size on sausage quality. Korean J. Food Sci. Anim. Resour. 26: 343-348 (2006)
  3. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. Korean J. Food Sci. Nutr. 32: 217-222 (2003)
  4. Kang TS, Kang MS, Sung JM, Kang AS, Shon HR, Lee SY. Effect of Pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Korean J. Mycol. 29: 86-90 (2001)
  5. Kawai, H, Sugahara T, Matsuzawa M, Sumiyashiki K, Aoyagi Y, Hosogai Y. Mineral contents in edible mushrooms. J. Jpn. Soc. Food Sci. Technol. 33: 250-255 (1986)
  6. Kawai H, Sugahara T, Fujishiro S, Matsuzawa M, Aoyagi Y, Hosogai Y. Mineral contents of edible mushrooms growing on wood. Comparison with mineral contents of mushrooms growing in soil. J. Jpn. Soc. Food Sci. Technol. 37: 468-473 (1990)
  7. Sasaki H, Aoyagi Y, Dasuga A, Tanaka Y, Masuzawa M, Kawai H. Relationships between fruit body compositions and substrates in bunashimeji [Hypsizygus marmoreus (peck) bigelow], nameko [Pholiota nameko (J. Ito)] and enokitake [Flammulina velutipes (Curt: Fr.) Sing.] mushrooms cultivated on sawdust substrate beds. J. Jpn. Soc. Food Sci. Technol. 42: 471-477 (1995)
  8. Sugahara T, Matsuzawa M, Fujishiro S, Aoyagi Y, Hosogai Y. Mineral contents in edible mushrooms growing in soil. J. Jpn. Soc. Food Sci. Technol. 37: 540-546 (1990)
  9. Tham LX, Matsuhashi S, Kume T. Growth and fruit body formulation of Ganoderuma lucidum on media supplemented with vanadium, selenium and germanium. Mycoscience 40: 87-92 (1999)
  10. Tham LX, Matsuhashi S, Kume T. Responses of Ganoderma lucidum to heavy metals. Mycoscience 40: 209-213 (1999)
  11. Yasui A, Tsutsumi C, Takasaki M, Mori T. Absorption of elements from heavy metals containing culture media by oyster mushroom (Pleurotus ostreatus (Fr.) Quel.). J. Jpn. Soc. Food Sci. Technol. 35: 160-165 (1998)
  12. Tabata T, Ogura T. Absorption of calcium and magnesium by the fruiting body of the cultivated mushroom Hypsizygus marmoreus (peck) bigelow from sawdust culture media. J. Food Sci. 68: 76-79 (2003)
  13. Tabata T, Shinohara H. Absorption of calcium salts added culture media by hiratake (Pleurotus ostreatus (Fr.) Quel.) and Nameko (Pholiota nameko J. Ito). J. Jpn. Soc. Food Sci. Technol. 42: 682-686 (1995)
  14. Lee NH, Im MH, Choi UK. Calcium absorption by the fruit body of saesongi mushroom. Food Sci. Biotechnol. 15: 308-311 (2006)
  15. Choi UK, Bajpai VK, Lee NH. Influence of calcinated starfish powder on growth, yield, spawn run and primordial germination of king oyster mushroom (Pleurotus eryngii). Food Chem. Toxicol. 47: 2830-2833 (2009)
  16. Perkin-Elmer Corporation. Analytical Methods for Atomic Absorption Spectroscopy. Perkin-Elmer Corp, Norwalk, CT, USA (1968)
  17. Liang Y, Chen H, Tang M, Shen S. Proteome analysis of an ectomycorrhizal fungus Boletus edulis under salt shock. Mycol. Res. 111: 939-946 (2007)
  18. Shimizu M, Wariishi H. Development of a sample preparation method for fungal proteomics. FEMS Microbiol. Lett. 247: 17-22 (2005)
  19. Jensen ON, Wilm M, Shevchenko A, Mann M. Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels. Methods Mol. Biol. 112: 513-530 (1999)
  20. Pappin DJ. Peptide mass fingerprinting using MALDI-TOF mass spectrometry. Methods Mol Biol. 64: 165-173 (1997)
  21. Harper JF, Sussman MR, Schaller GE, Putnam-Evans C, Charbonneau H, Harmon AC. A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951-954 (1991)
  22. Jouaville LS, Pinton P, Bastianutto C, Rutter GA, Rizzuto R. Regulation of mitochondrial ATP synthesis by calcium: Evidence for a long-term metabolic priming. Proc. Natl. Acad. Sci. USA 96: 13807-13812 (1999)
  23. Galperin MY, Bairoch A, Koonin EV. A superfamily of metalloenzymes unifies phosphopentomutase and cofactor-independent phosphoglycerate mutase with alkaline phosphatases and sulfatases. Protein Sci. 7: 1829-1835 (1998)
  24. Fothergill-Gilmore LA, Watson HC. The phosphoglycerate mutases. Adv. Enzymol. RAMB 62: 227-313 (1989)
  25. Pohl M, Sprenger GA, Mller M. A new perspective on thiamine catalysis. Curr. Opin. Biotech. 15: 335-342 (2004)
  26. Machado CR, de Oliveira RL, Boiteux S, Praekelt UM, Meacock PA, Menck CF. Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair. Plant Mol. Biol. 31: 585-593 (1996)
  27. Ahn IP, Kim S, Lee YH. Vitamin B1 functions as an activator of plant disease resistance. Plant Physiol. 138: 1505-1515 (2005)