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Mucosal Immunoadjuvant Activity of Korean Mistletoe Lectin-C

한국산 겨우살이 렉틴의 경구투여에 의한 항원 특이적 점막면역 증진 효과

  • Kim, Jin-Chul (Institute for Biomedical Research, Handong University) ;
  • Yoon, Taek-Joon (Department of Food & Nutrition, Yuhan University) ;
  • Song, Tae-Jun (Institute for Biomedical Research, Handong University) ;
  • Kim, Young-Hoon (Institute for Biomedical Research, Handong University) ;
  • An, Hyo-Sun (Institute for Biomedical Research, Handong University) ;
  • Kim, Jong-Bae (Institute for Biomedical Research, Handong University)
  • 김진철 (한동대학교 생의학연구소) ;
  • 윤택준 (유한대학 식품영양과) ;
  • 송태준 (한동대학교 생의학연구소) ;
  • 김영훈 (한동대학교 생의학연구소) ;
  • 안효선 (한동대학교 생의학연구소) ;
  • 김종배 (한동대학교 생의학연구소)
  • Received : 2010.10.08
  • Accepted : 2010.11.15
  • Published : 2011.02.28

Abstract

The adjuvant effects of Korean mistletoe lectin-C (KML-C) were investigated following the oral administration of KML-C with ovalbumin (OVA) as an antigen. Mice were orally immunized with OVA alone or admixed with various doses of KML-C or cholera toxin (CT), and the titer of OVA-specific antibody in the serum and mucosal secretions were determined. OVA+KML-C-treated mice showed high titers of IgA specific to CT in mucosal secretions. The antibody titers in the serum of OVA+KML-C-treated mice were comparable to those in the serum of OVA+CT-treated mice. When mice were immunized with OVA+KML-C or with CT alone and subsequently injected with OVA on the footpads after the primary immunization, they showed a more significant increase in delayed-type hypersensitivity reactions than when they were administered CT alone. These results suggest that KML-C is a potent immunoadjuvant that enhances both humoral and cellular immunity by the mucosal immune system.

Keywords

mistletoe lectin-c;mucosal immune response;immunoadjuvant;oral administration;IgA

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