Microbiological Quality of Fresh-Cut Produce and Organic Vegetables

신선편의 샐러드와 유기농 채소류의 미생물학적 품질 및 식중독 미생물 오염도

  • Jo, Mi-Jin (Food Safety Research Division, Korea Food Research Institute) ;
  • Jeong, A-Ram (Food Safety Research Division, Korea Food Research Institute) ;
  • Kim, Hyun-Jung (Food Safety Research Division, Korea Food Research Institute) ;
  • Lee, Na-Ri (Food Safety Research Division, Korea Food Research Institute) ;
  • Oh, Se-Wook (Department of Food Science and Human Nutrition, Kookmin University) ;
  • Kim, Yun-Ji (Food Safety Research Division, Korea Food Research Institute) ;
  • Chun, Hyang-Sook (Food Safety Research Division, Korea Food Research Institute) ;
  • Koo, Min-Seon (Food Safety Research Division, Korea Food Research Institute)
  • 조미진 (한국식품연구원 안전성 연구단) ;
  • 정아람 (한국식품연구원 안전성 연구단) ;
  • 김현정 (한국식품연구원 안전성 연구단) ;
  • 이나리 (한국식품연구원 안전성 연구단) ;
  • 오세욱 (국민대학교 식품영양학과) ;
  • 김윤지 (한국식품연구원 안전성 연구단) ;
  • 전향숙 (한국식품연구원 안전성 연구단) ;
  • 구민선 (한국식품연구원 안전성 연구단)
  • Received : 2010.05.18
  • Accepted : 2010.10.18
  • Published : 2011.02.28


This study was performed to assess the microbiological quality and potential health risk of fresh-cut produce and organic vegetables sampled from supermarkets and department stores in Korea. A total of 96 samples comprised three types of fresh-cut produce (sprouts, mixed-vegetables, fruit) and three types of organic vegetables (lettuce, perilla leaf, green pepper). The samples were analyzed for total viable cell counts, coliforms, Enterobacteriaceae, Escherichia coli, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus, and Staphylococcus aureus. The microbiological counts of fruit were very low. Sprouts were highly contaminated by total viable cell counts ($8.3{\pm}0.57$ log CFU/g), Enterobacteriaceae ($7.1{\pm}0.76$ log CFU/g), and coliforms ($4.9{\pm}0.40$ log MPN/g), and showed a high incidence level of B. cereus ($2.9{\pm}0.48$ log CFU/g). Of the fresh-cut produce analyzed, six (13.6%) mixed-vegetable salads were E. coli positive. S. aureus was detected in only one sprout sample and one mixed-vegetable salad, and its contamination levels were under 2 log CFU/g, which is appropriate for Korean standards (<3 log CFU/g) of fresh-cut produce. Of the organic vegetables, lettuces were highly contaminated by total viable cell counts ($6.4{\pm}0.74$ log CFU/g), Enterobacteriaceae ($5.7{\pm}0.98$ log CFU/g), and coliforms ($3.7{\pm}1.72$ log MPN/g). Two (13.6%) organic lettuce and one (7.1%) perillar leaf sample were E. coli positive, and S. aureus was detected in one lettuce and two perilla leaf samples. Salmonella spp., Vibrio parahaemolyticus, and Listeria monocytogenes were not detected in any of the fresh-cut produce or organic vegetables analyzed.


microbiological quality;fresh-cut produce;organic vegetable;Enterobacteriaceae;Bacillus cereus


Supported by : 한국식품연구원


  1. Abadias M, Usall J, Anguera M, Solsona C, Vinas I. Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. Int. J. Food Microbiol. 123: 121-129 (2008)
  2. Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim GS, Ha SD. Microbial contamination levels of fresh vegetables distributed in markets. J. Fd Hyg. Safety 20: 43-47 (2005)
  3. Chia-Min L, Fernando SY, Cheng-I W. Occurrence of Listeria monocytogenes, Salmonella spp., Escherichia coli and E. coil O157:H7 in vegetable salads. Food Control 7: 13-140 (1996)
  4. Lee YS, Kim S, Kim DH. Current status of fresh-cut market in Korea and stimulus measures. Korea Rural Economic Institute Report 602: 16-19 (2009)
  5. Kim DM, Hong SI. Current status and prospect of fresh-cut produce in Korea. Food Preserv. Proc. Ind. 4: 41-61 (2004)
  6. Park HO, Kim CM, Woo GJ, Park SH, Lee DH, Chang EJ, Park KH. Monitoring and trend analysis of food poisoning outbreaks occurred in recent years in Korea. J. Fd Hyg. Safety 16: 280-294 (2001)
  7. Garcia-Gimeno RM, Zurera-Cosano G, Amaro-Lopez M. Incidence, survival and growth of Listeria monocytogenes in readyto- use mixed vegetable salads in Spain. J. Food Safety 16: 75-86 (1996)
  8. Beuchat LR, Harris LR, Linda J, Ward TE, Kajs TM. Development of a proposed standard method for assessing the efficacy of fresh produce sanitizer. J. Food Protect. 64: 1103-1109 (2001)
  9. Harris LJ, Beuchat LR, Kajs TM, Ward TE, Taylor CJ. Efficacy and reproducibility of a produce wash in killing Salmonella on the surface of tomatoes assessed with a proposed standard method for produce sanitizers. J. Food Protect. 64: 1477-1482 (2001)
  10. Itoh Y, Sugita-Konishi Y, Kasuage F, Iwaki M, HaraHudo Y, Saito N, Noguchi Y, Konuma H, Kumagai S. Enterohomorrhagic Esherichia coli O157:H7 present in radish sprouts. Appl. Environ. Microb. 64: 1352-1535 (1998)
  11. Hedberg CW, Angulo FG, White KE, Langkop CW, Schell WL, Stobierski MG, Schuchat A, Besser JM, Dietrich S, Helsel L, Griffin PM, McFarland JW, Osterhorm MT. Outbreaks of Salmonellosis associated with eating uncooked tomatoes. Implications for Public Health. The Investigation Team. Epidemiol. Infect. 122: 385-393 (1999)
  12. Seo JE, Lee JK, Oh SW, Koo MS, Kim YH, Kim YJ. Changes of microorganisms during fresh-cut cabbage processing: Focusing on the changes of air-borne microorganisms. J. Fd Hyg. Safety 22: 288-293 (2007)
  13. Kim JS, Bang OK, Ghang HC. Examination of microbiological contamination of ready-to-eat vegetable salad. J. Fd Hyg. Safety 19: 60-65 (2004)
  14. KFDA, Food Code. 10-3-1-43. Korea Food and Drug Administration. Seoul, Korea (2009)
  15. Lammerding AL. An overview of microbial food safety risk assessment. J. Food Protect. 60: 1420-1425 (1997)
  16. Won YJ, Yoon CY, Seo IW, Nam HS, Lee DM, Park DH, Lee HM, Kim SS, Lee KY. The study for the occurrence of food poisoning bacteria in organic vegetables. Annu. Rep. KFDA, Seoul, Korea 6: 521 (2002)
  17. ISO. ISO 21528-2: 2004, Microbiology of food and animal feeding stuffs-Horizontal methods for detection and enumeration of Enterobacteriaceae. International Organization for Standardization (2004)
  18. Solberg M, Buchalew JJ, Chen CM, Schaffner DW, O'Neill K, McDowell J, Post LS, Boderck M. Microbial safety assurance system for foodservice facilities. Food Technol., 44: 68-72 (1990)
  19. Health Protection Agency. Identification of Enterobacteriaceae. National Standard Method BSOPID 16, Issue 2. Accessed Feb. 10, 2010.
  20. European Union Regulation. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on Microbiological criteria for foodstuffs. Off. J. Eur. Union L338, 22: 1-26 (2005)
  21. Choi JW, Kim YJ, Lee JK, Kim YH, Kwon KS, Hwang IG, Oh SW. Multiple confirmation and RAPD-genotyping of Enterobacter sakazakii isolated from Sunsik. Korean J. Food Sci. Technol. 40: 101-105 (2008)
  22. Jung DS, Shin DH, Jung DH, Kim CM, Lee IS. Food Hygienics. Jungmoonkak, Seoul, Korea. pp. 22-23 (2002)
  23. Kim HJ, Hwang YI, Lee SC. Inhibitory effect of hydrogen peroxide on the growth of Escherichia coli. J. Basic Sci. 19: 113-117 (2004)
  24. Seo KY, Lee MJ, Yeon JH, Kim IJ, Ha JH, Ha SD. Microbiological contamination levels of in salad and side dishes distributed in markets. J. Fd Hyg. Safety 21: 263-268 (2006)
  25. Jung MS. Study on the risk management for risk reduction of Stapylococcus aureus in ready-to-eat foods. Annu. Rep. KFDA, Korea 11: 525-527 (2007)
  26. Shim SB, Ham SN, Kwoun PS, Lee SO, Kim SH, Lee GW, Bang OK. Monitoring contamination of vegetables salad and study in reduction for food poisoning. Annu. Rep. KFDA, Seoul, Korea 7: 364-365 (2003)
  27. Walls I, Scatt VN. Use of predictive microbiology in microbial food safety risk assessment. Int. J. Food Microbiol. 36: 97-102 (1997)
  28. Wher MH. Listeria monocytogenes-A current dilemma. J. Assoc. Off. Anal. Chem. 70: 769 (1987)
  29. Donnelly CW, Briggs EH. Psychrotropic growth and thermal inactivation of Listeria monocytogenes as a function of milk composition. J. Food Protect. 49: 994-998 (1986)
  30. Koo M. Bacillus cereus-An ambusher of food safety. Bull. Food Technol. 22: 587-600 (2009)
  31. EFSA. Opinion of the scientific panel on biological hazards on Bacillus cereus and other Bacillus spp. In foodstuffs. EFSA J. 175: 1-48 (2005)
  32. Park SY, Choi JW, Yeon JH, Lee MJ, Lee DH, Kim KS, Park KH, Ha SD. Assessment of contamination levels of foodborne pathogens isolated in major RTE foods marketed in convenience stores. Korean J. Food Sci. Technol. 37: 274-278 (2005)
  33. Gilbert RJ, Humphrey TJ. Food-borne bacterial gastroenteritis. In: Hausler WJ, Sussman M (eds), Topley and Wilson's Microbiology and microbial infection 9th edn. Vol. 3. Bacterial Infection. pp. 539-565. Hodder Arnold Pub., London, UK (1998)

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