DOI QR코드

DOI QR Code

Comparative Studies on the Acute Toxicities of Whole Solids and Solids Aqueous Extracts Based on the Inhibition of Bacterial Bioluminescence Production

  • Kong, In-Chul (Department of Environmental Engineering, Yeungnam University)
  • Received : 2010.05.13
  • Accepted : 2011.03.03
  • Published : 2011.03.30

Abstract

The aim of this investigation was to demonstrate a rapid bioluminescence bioassay for comparison of the toxicity of whole solids and the aqueous extracts of various environmental solid samples. With regard to the toxicities, those for the soil extracts were mostly found to be lower than those of whole soils, which may have been caused by un-extracted pollutants or dilution during the extraction process. Solid samples from dam-reservoir sediments and municipal refuses were also tested. The toxicities of the solid extracts (0-34%; refuses and sediments) were much lower than those of the whole solids (13-91%). The bioluminescence inhibition test indicated that the harmful effects of the contaminated solids samples were greater than those of the solid extracts.

References

  1. Eggen RI, Behra R, Burkhardt-Holm P, Escher BI, Schweigert N. Challenges in ecotoxicology. Environ. Sci. Technol. 2004;38:58A-64A. https://doi.org/10.1021/es040349c
  2. Leitgib L, Kálmán J, Gruiz K. Comparison of bioassays by testing whole soil and their water extract from contaminated sites. Chemosphere 2007;66:428-434. https://doi.org/10.1016/j.chemosphere.2006.06.024
  3. Ahtiainen J, Valo R, Järvinen M, Joutti A. Microbial toxicity tests and chemical analysis as monitoring parameters at composting of creosote-contaminated soil. Ecotoxicol. Environ. Saf. 2002;53:323-329. https://doi.org/10.1006/eesa.2002.2225
  4. Robidoux PY, Gong P, Sarrazin M, et al. Toxicity assessment of contaminated soils from an antitank firing range. Ecotoxicol. Environ. Saf. 2004;58:300-313. https://doi.org/10.1016/j.ecoenv.2003.11.004
  5. Choi SH, Gu MB. Toxicity biomonitoring of degradation byproducts using freeze-dried recombinant bioluminescent bacteria. Anal. Chim. Acta 2003;481:229-238. https://doi.org/10.1016/S0003-2670(03)00091-6
  6. Ren S, Frymier PD. Toxicity of metals and organic chemicals evaluated with bioluminescence assays. Chemosphere 2005;58:543-550. https://doi.org/10.1016/j.chemosphere.2004.07.005
  7. Eldridge ML, Sanseverino J, Layton AC, Easter JP, Schultz TW, Sayler GS. Saccharomyces cerevisiae BLYAS, a new bioluminescent bioreporter for detection of androgenic compounds. Appl. Environ. Microbiol. 2007;73:6012-6018. https://doi.org/10.1128/AEM.00589-07
  8. Gu MB, Gil GC. A multi-channel continuous toxicity monitoring system using recombinant bioluminescent bacteria for classification of toxicity. Biosens. Bioelectron. 2001;16:661-666. https://doi.org/10.1016/S0956-5663(01)00195-6
  9. Kong IC, Kim M, Bhandari A. Aqueous phase toxicity changes resulting from horseradish peroxidase-mediated polymerization of phenols and hydroxylated polynuclear aromatic contaminants. Bull. Environ. Contam. Toxicol. 2007;79:104-108. https://doi.org/10.1007/s00128-007-9146-2
  10. Scheers EM, Van der Wielen C, Dierickx PJ. Toxicological evaluation of waste-water samples to appropriately sensitized cultured fathead minnow cells compared with the microtox assay. Bull. Environ. Contam. Toxicol. 2002;68:253-260. https://doi.org/10.1007/s001280246
  11. Lajoie CA, Lin SC, Nguyen H, Kelly CJ. A toxicity testing protocol using a bioluminescent reporter bacterium from activated sludge. J. Microbiol. Methods 2002;50:273-282. https://doi.org/10.1016/S0167-7012(02)00042-8
  12. Ma LQ, Rao GN. Chemical fractionation of cadmium, copper, nickel, and zinc in contaminated soils. J. Environ. Qual. 1997;26:259-264.
  13. Bitton G, Garland E, Kong IC, Morel JL, Koopman B. A direct solid-phase assay specific for heavy metal toxicity. I. Methodology. Soil Sediment Contam. 1996;5:385-394. https://doi.org/10.1080/15320389609383536
  14. Dave G. Sediment toxicity in lakes along the River Kolbacksan, central Sweden. Hydrobiologia 1992;235-236:419-433. https://doi.org/10.1007/BF00026232
  15. Campbell M, Bitton G, Koopman B. Toxicity testing of sediment elutriates based on inhibition of alpha-glucosidase biosynthesis in Bacillus licheniformis. Arch. Environ. Contam. Toxicol. 1993;24:469-472. https://doi.org/10.1007/BF01146163
  16. Dutka BJ, Nyholm N, Petersen J. Comparison of several microbiological toxicity screening tests. Water Res. 1983;17:1363-1368. https://doi.org/10.1016/0043-1354(83)90265-8
  17. Castillo GC, Vila IC, Neild E. Ecotoxicity assessment of metals and wastewater using multitrophic assays. Environ. Toxicol. 2000;15:370-375. https://doi.org/10.1002/1522-7278(2000)15:5<370::AID-TOX3>3.0.CO;2-S