DOI QR코드

DOI QR Code

Synchronization of Cell Cycle in Korean Hydrogen Producing Cyanobacterial Strains

한국산 수소생산 남세균 종주들의 세포주기 동조화

  • Park, Jong-Woo (Department of Oceanography, Kunsan National University) ;
  • Ahn, Se-Hee (Department of Oceanography, Kunsan National University) ;
  • Kim, Hyung-Seop (Research Institute of Fisheries Technology) ;
  • Yih, Won-Ho (Department of Oceanography, Kunsan National University)
  • Received : 2011.09.29
  • Accepted : 2011.10.21
  • Published : 2011.10.30

Abstract

Under a daily photoperiod of 14h light and 10h dark synchronization of cell cycle in Korean Cyanothece spp. strains and $Synechococcus$ sp. strain Miami BG043511 was analyzed as to be applicable to enhanced hydrogen production. For all strains peaks of double cell were observed during the light period of a daily cycle. Peaks of maximal cell size measured by a coulter counter appeared at the peak of double cells observed under light microscope reconfirming the synchronization of daily cell cycle. The cell cycle synchronization became weakened within two days when treated with continuous illumination. Rapid detection of the peak time of double cell percentage by coulter counters may contribute to quasi-realtime feedback control for efficient production of photobiological hydrogen by unicellular cyanobacterial strains.

Acknowledgement

Grant : 해양독성생물자원 기탁등록보존기관

Supported by : 국토해양부

References

  1. 김재만 : "한국 연안산 질소고정 남세균 종주들의 세포주기 동조화와 광생물학적 수소생산능력", 군산대학교 대학원, 석사학위논문, 2008, p. 52.
  2. 박종우 : "한국 연안산 남세균 종주들의 광생물학적 수소생산능력", 군산대학교 대학원, 석사학위논문, 2007, p. 53
  3. 박종우, 이원호 : "국내 연안산 질소고정 남세균 종주의 세포농도별 광생물학적 수소생산력", 2009 한국수소 및 신에너지학회 추계 학술대회 논문집, 2009, pp. 80-83.
  4. S. Golden, M. Ishiura, C. Johnson, T. Kondo : "Cyanobacterial circadian rhythms", Annual Review of Plant Physiology and Plant Molecular Biology. 48, 1997, pp. 327-354. https://doi.org/10.1146/annurev.arplant.48.1.327
  5. R. Guillard : "Methods for microflagellates and nanoplankton", In, Handbook of phycological methods, 1973, pp. 69-85.
  6. H. Iwazaki, T. Kondo : "The current state and problems of circadian clock studies in cyanobacteria", Plant cell Physiology, 41(9), 2000. 99. 1013-1020. https://doi.org/10.1093/pcp/pcd024
  7. Y. Luo, S. Kumazawa, L. Brand : "Effect of exogenous substrates on hydrogen photoproduction by a mirine cyanobacterium, Synechococcus sp. MiamiBG043511", Biohydrogen. 1998, pp. 219-225.
  8. A. Mitsui, S. Cao, A. Takahashi, T. Arai : "Growt h synchrony and cellular parameters of the unicellular nitrogen-fixing marine cyanobacterium, Synechococcus sp. strain Miami BG 043511 under continuous illumination", Physiologia Plantarum 69, 1987, pp. 1-8. https://doi.org/10.1111/j.1399-3054.1987.tb01938.x
  9. A. Mitsui, S. Kumazawa : "Hydrogen production by marine photosynthetic organisms as a potential energy resource", Biological solar energy conversion, 1977, pp. 23-51.
  10. A. Mitsui, S. Kumazawa : "Nitrogen fixation by synchronously grown unicellular aerobic nitrogenfixing cyanobacteria", In: Methods in enzymology, edited by Packer, L. and A. N. Glazer, Academic Press, Vol. 167, 1988, pp. 484-490.
  11. J. Park, J. Kim, N. Ha, W. Yih : "Synchronization of cell cycle in a cultured marine unicellular $N_{2}$-fixing cyanobacterium, Cyanothece sp. KNU CB-MAL031", The Yellow Sea, 10, 2009, pp. 19-23.
  12. S. Suda, A. Mitsui : "Alternative and cyclic appearance of $H_{2}$ and $O_{2}$ photoproduction activities under non-growing $H_{2}$ production conditions in an aerobic nitrogen-fixing cyanobacterium Synechococcus sp.", Current Microbiology, 30, 1995, pp. 1-6. https://doi.org/10.1007/BF00294515