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Hydrogen Production from Hyperthermophilic Archaebacteria Thermococcus onnurineus NA1

초고온성 고세균 Thermococcus onnurineus NA1에 의한 수소생산

  • Kim, Ok-Sun (Wastes Energy Research Center, Korea Institute of Energy Research) ;
  • Na, Jeong-Geol (Wastes Energy Research Center, Korea Institute of Energy Research) ;
  • Kim, Hae-Jin (Ensoltek) ;
  • Rhee, Young-Woo (Graduate School of Green Energy Technology, Chungnam National University) ;
  • Kim, Mi-Sun (Wastes Energy Research Center, Korea Institute of Energy Research)
  • 김옥선 (한국에너지기술연구원 폐자원에너지센터) ;
  • 나정걸 (한국에너지기술연구원 폐자원에너지센터) ;
  • 김해진 ((주)엔솔테크) ;
  • 이영우 (녹색에너지기술전문대학원) ;
  • 김미선 (한국에너지기술연구원 폐자원에너지센터)
  • Received : 2011.02.08
  • Accepted : 2011.10.21
  • Published : 2011.10.30

Abstract

A hyperthermophilic archaeon, $Thermococcus$ $onnurineus$ NA1 was studied to investigate its fermentation characteristics using various carbon sources including formate, maltose and carbon monoxide during the anaerobic batch cultivation at $80^{\circ}C$. Formate was the best carbon source for cell growth and hydrogen production among others. In the batch culture on formate, it was found that the cell concentration increased exponentially by 12 hrs of culture, after which the cell growth and formate consumption was retarded. Hydrogen production was continued more than 24 hrs although the cell growth was ceased at 18 hrs. Hydrogen production rate was directly correlated with the cell growth and formate degradation up to 18 hrs, and the average hydrogen production yield was 1.05 mole-$H_2$/mole-formate. Cell growth and hydrogen production were optimized at the initial pH 6-7, while inhibited at the initial pH lower than 5 and higher than 9.

Acknowledgement

Grant : 해양 고세균이용바이오 수소생산기술개발

Supported by : 국토해양부

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