- Volume 24 Issue 2
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Concentrations of Calcium-binding Protein and Bone Gla-protein in Culture Medium and CaBP mRNA Expression in Osteoblasts of Broiler Chickens
- Guo, Xiaoyu (College of Animal Science, Inner Mongolia Agricultural University) ;
- Yan, Sumei (College of Animal Science, Inner Mongolia Agricultural University) ;
- Shi, Binlin (College of Animal Science, Inner Mongolia Agricultural University) ;
- Feng, Yongmiao (Huhhot Vocational College)
- Received : 2010.02.17
- Accepted : 2010.07.09
- Published : 2011.02.01
This study was conducted to determine the effects of excess vitamin A on alkaline phosphatase (ALP) activity, contents of calcium-binding protein (CaBP), bone gla-protein (BGP) in culture medium and CaBP mRNA expression in chicken osteoblasts in vitro. Osteoblastic cells in the tibia from 1-day-old Arbor Acre broiler chickens were isolated using enzyme digestion. The subconfluenced cells were divided into eight treatments with six replicates in each treatment and cultured in a medium containing either vehicle or different levels of vitamin A (0, 0.2, 0.6, 1.0, 2.0, 5.0, 10.0 and
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