Effect of Antioxidants on Physio-biochemical and Hematological Parameters in Broiler Chicken at High Altitude

  • Biswas, A. (Defence Institute of High Altitude Research (DIHAR)) ;
  • Ahmed, M. (Defence Institute of High Altitude Research (DIHAR)) ;
  • Bharti, V.K. (Defence Institute of High Altitude Research (DIHAR)) ;
  • Singh, S.B. (Defence Institute of High Altitude Research (DIHAR))
  • Received : 2010.02.17
  • Accepted : 2010.07.09
  • Published : 2011.02.01


The present study was carried out on broilers to study the effect of oral administration of vitamin E and selenium (E-care Se) on growth performance, haematological and biochemical parameters for a period of 42 days (6 weeks). A total of 90 oneday-old broiler chicks were divided into three equal groups: $T_1$, $T_2$ and $T_3$. Group T1 was maintained as control and was fed only with the basal diet throughout the experimental period. Two experimental diets, $T_2$ and $T_3$, were formulated to contain an additional 100 g (150 IU vitamin E/kg+0.5 mg Se/kg) and 200 g (300 IU vitamin E/kg+1.0 mg Se/kg) of E-care Se which was the source of vitamin E and selenium. Body weight was significantly (p<0.05) higher in antioxidant-treated groups compared to the control group. There were no significant differences in feed conversion ratio (FCR). Blood samples were collected from the jugular vein for haematological (TEC, Hb, PCV and ESR) and biochemical (GOT and GPT) study. Body weight was increased significantly in both treated groups compared with the control group and highest body weights were recorded in group $T_2$. TEC, PCV and Hb content increased significantly (p<0.01) in the treated groups as compared to the control group, but ESR, GOT and GPT values decreased significantly (p<0.01) in both treated groups as compared to the control group. The result reveals that use of antioxidants (vitamin E and selenium) is an effective way of getting the best result in terms of body weight gain and haemato-biochemical profiles in broiler birds at high altitude.


Antioxidants;Vitamin E;Selenium;High Altitude;Broiler Chicken


  1. Biswas, A., J. Mohan, K. V. H. Sastry and J. S. Tyagi. 2008. Effect of higher levels of dietary vitamin E on performance and immune response in growing Japanese quail. J. Appl. Anim. Res. 33:61-64.
  2. Duncan, B. D. 1955. Multiple range and multiple F tests. Biometrics 11:1-12.
  3. Dukes, H. H. 1955. The physiology of domestic animals. 7th edition Bailers Tindal and Co. London.
  4. Guo, Y. N., Q. Tang, J. M. Yuan and Z. R. Jiang. 2001. Effect of supplementation of vitamin E on the performance and tissue peroxidation of broiler chicks and the stability of thigh meat oxidative deterioration. Anim. Feed Sci. Technol. 89:165-173.
  5. Hill, E. W., M. L. Scott, L. C. Norris and G. F. Henser. 1961. Reinvestigation of the vitamin A and D requirements of laying and breeding hens and their progeny. Poult. Sci. 40:1245-1247.
  6. Kumar, J. S. and P. Rawat. 1976. Effect of vitamin on growth performance and biochemical parameters. Indian J. Anim. Sci. 45:135-138.
  7. Lin, Y. F., S. J. Chang and A. L. Hsu. 2004. Effects of supplemental vitamin E during the laying period on the reproductive performance of Taiwan native chickens. Br. Poult. Sci. 45(6):807-814.
  8. NRC. b. Nutrient Requirements of Poultry, 8th ed., Washington, USA: National Research Council, National Academy of Science.
  9. Pravbhakaran, V., S. Chithravel, K. Prabbha and C. S. Saravarna. 1996. Hematological and biochemical profile of white leghorn chickens. Indian J. Anim. Health 35:11-15.
  10. Reitman, S. and S. Frankel. 1957. A calorimetric method for determination of glutamic oxaloacetic and glutamic pyruvic transaminase activities. J. Dairy Sci. 48:1684-1687.
  11. Sahin, N., K. Sahin and O. Kucuk. 2001. Effect of vitamin E and vitamin A supplementation on performance, thyroid status and serum concentrations of some metabolites and minerals in broilers reared under heat stress (32${\circ}$C). Vet. Med. 46(11- 12):286-292.
  12. Shastry, G. A. 1983. Veterinary clinical pathology. 2nd edn., CBS Publishers and Distributors, New Delhi.
  13. Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods, 8th edn. Oxford and IBH Publishing Co., Kolkata, India.
  14. Swain, B. K. 1996. Influence of certain nutrients and their interaction on the performance and immune response of broilers. Ph.D. Thesis, IVRI, Deemed University, Izatnagar, India.
  15. Swain, B. K. and T. S. Johri. 2000. Effects of supplementation of combinations of different levels of selenium and vitamin E on relative weight of some organs and serum enzymes level in broilers. Indian J. Poult. Sci. 35(1):66-69.
  16. Villar-Patino, G., A. Diaz-Cruz, E. Avila-Gonzalez, R. Guinzberg, J. L. Pablos and E. Pina. 2002. Effects of dietary Supplementation with vitamin C or vitamin E on cardiac lipid peroxidation and growth performance in broilers. Am. J. Vet. Res. 63(5):673-676.

Cited by

  1. The effect of dietary bacterial organic selenium on growth performance, antioxidant capacity, and Selenoproteins gene expression in broiler chickens vol.13, pp.1, 2017,
  2. Influences of dietary supplementation of peanut skin powder (Arachis Hypogaea) on growth performance, carcass traits, blood chemistry, antioxidant activity and meat quality of broilers vol.58, pp.5, 2018,
  3. Wheat germ oil enrichment in broiler feed with α-lipoic acid to enhance the antioxidant potential and lipid stability of meat vol.12, pp.1, 2013,