A Rapid Method for Monitoring of Gram-positive Bacteria in Wastewater Treatment Systems

폐수처리시스템에서의 그람 양성 세균 모니터링 방법

  • Nam, Ji-Hyun (Department of Microbiology, Chungbuk National University) ;
  • Bae, Woo-Keun (Department of Civil and Environmental Engineering, Hanyang University) ;
  • Lee, Dong-Hun (Department of Microbiology, Chungbuk National University)
  • Received : 2011.03.14
  • Accepted : 2011.03.22
  • Published : 2011.03.31


A simple and rapid method was developed for monitoring of Gram-positive bacteria in the wastewater treatment system. Culture suspensions of 4 Gram-positive and 4 Gram-negative strains were filtrated and stained with a polyethersulfone membrane filter and Toluidine Blue-O. To establish quantitative color image analysis, the intensity value of RGB (red-green-blue) color of a scanned filter image was analyzed with a photographic program. Red and green color values of Gram-positive bacteria were higher than those of Gram-negative bacteria. This method was applied to the activated sludge mixed with the Gram-positive bacteria. Although evaluation was difficult due to the irregular size and shape of flocs, the population of Gram-positive bacteria in the activated sludge could be monitored with floc dispersion technique. The more amounts of Gram-positive bacteria in the activated sludge led to the increase of red and green color values. This method provides a rapid and quantitative measurement of Gram-positive bacteria within the wastewater treatment systems.


Supported by : 충북대학교


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