Inhibition of Adipogenesis in 3T3-Ll Adipocytes with Water and Ethanol Extracts of Cudrania tricuspidata Leaves

꾸지뽕잎(Cudrania tricuspidata) 추출물의 3TS-L1 세포 분화 억제

  • Received : 2010.11.12
  • Accepted : 2011.03.25
  • Published : 2011.04.30


The inhibitory effects of adipogenesis in 3T3-L1 adipocytes with water and ethanol extracts of Curdrania tricuspidata leaves were investigated. The lipid accumulation of ethanol extract from Curdrania tricuspidata leaves at a concentration of 5 mg/mL was reduced by 50% compared with control cells, whereas water extracts reduced by 86%. The survival rate of cell viability test showed 80% in the growth of cells at concentrations of 0.5-5 mg/mL. The contents of DNJ(1-deoxynojirimycin) and rutin were higher in leaves than those of stem. Moreover, DNJ contents were detected in large amounts in ethanol extract ($3921.3{\mu}g/g$) compared with water extract ($2906.3{\mu}g/g$). On the contrary, the rutin contents was detected in large amounts in water extracts compared with ethanol extract. These results indicate that Curdrania tricuspidata leaves extract is potential sources of natural antiobesity agent.


3T3-L1;1-deoxynojirimycin;adipocyte;cell viability;Cudrania tricuspidata


  1. Kopelman PG (2000) Obesity as a medical problem. Nature, 404, 635-643
  2. Green H and Kehinde O (1974) Sublines of mouse 3T3 cells that accumlate lipid. Cell, 1, 113-116
  3. Otto TC and Lane MD (2005) Adipose development from stem cell to adipocyte. Crit Rev Biochem Mol Biol, 40, 229-242
  4. Kim SH, Kim NJ, Chon JS and Park JC (1993) Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau. J Korean Soc Food Sci Nutr, 22, 68-72
  5. Otlersen T, Vance B, Doorenbos NJ, Chang BL and EI-Feraly FS (1977) The crystal structure of cudranone, 2,6,3'-trihydroxy-4-methoxy-2'-(3-methoxy-2-buternyl)-I, a new antimicrobial agent from Cudrania chochinchinensis. Acta Chem Scand, 31, 434-436
  6. Chang CH, Lin CC, Hattori M and Namba T (1994) Effects of anti-lipid peroxidation of Cudrania cochinchinensis var. gerontogea. J Ethnopharomacol, 44, 179-185.
  7. Joo HY and Lim KT (2009) Protective effect of glycoprotein isolated from Cudrania tricuspidata on liver in CCl4-treated A/J mice. Korean J Food Sci Technol, 41, 93-99
  8. Kim SH, Kim NJ, Choi JS and Park JC (1993). Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata bureau. J Korean Soc Food Nutr, 22, 68-72
  9. Niwa T, Inouye T, Koaze TY and Niida T (1970) Nojirimycin as a potent inhibitor of glucosidase. Agr Biol Chem, 34, 966-971
  10. Cho YS, Park SP, Lee JY, Kang KD and Hwang KY (2008) Hypoglycemic effect of culture broth of Bacillus subtilis S10 producing 1-deoxynojirimycin. J Korean Soc Food Sci Nutr, 37, 1401-1407
  11. Yoo SK, Kim MJ, Kim JW and Rhee SJ (2002) Effects of YK-209 mulberry leaves on disaccharidase activites of small intestine and blood glucose- lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 31, 1071-1077
  12. Kim HB and Kim SL (2004) Quantification and varietal variation of rutin in mulberry fruits. Korean J Seric Sci, 46, 1-5
  13. Lee JS, Son SS, Maeng YS and Ju IS (1994) Effects of buckwheat on organ weight glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J Nutr, 27, 819-827
  14. Lee EH and Kim CJ (2008) Nutritional changes of buckwheat during germination. Korean J Food Cultu, 23, 121-129
  15. Chin HS, Park KJ, Pack SH and Kim JK (2009) The effects of herbal extract mixture on anti-obesity. J Korean Soc Food Sci Nutr, 38, 32-38
  16. Cha SY, Jang JY, Lee YH, Lee G, Lee HJ, Hwang KT, Kim Y, Jun W and Lee J (2010) Lipolytic effect of methanol extracts from Luffa sylindrica in mature 3T3-L1 adipocytes. J Korean Soc Food Sci Nutr, 39, 813-819
  17. Green H and Meuth M (1974) An established pre-adipose cell line and its differentiation in culture. Cell 3, 127-133.
  18. Cho Y, Lee SM, Kim Y, Jeon G, Sung J, Jeong HS and Lee J (2010) Defatted grape seed extracts suppress adipogenesis in 3T3-L1 preadipocytes. J Korean Soc Food Sci Nutr, 39, 927-931
  19. Hwang EY, Hong JH, Choi JH, Lee EJ and Lee IS (2009) Study on anti-obesity and hypoglycemic effects of Lycium chinense mill extracts. J Korean Soc Food Sci Nutr, 38, 1528-1534
  20. Ko BS, Jun DW, Jang JS, Kim JH and Park S (2006) Effect of sasa borealis and white lotus roots and leaves on insulin action and secretion in vitro. Korean J Food Sci Technol, 38, 114-120

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