Effects of Supplementary Copper Sources (Cu Sulfate, Cu-Methionine, Cu-Soy Proteinate) on the Performance Broiler Chickens

구리 공급원들(Cu Sulfate, Cu-Methionine, Cu-Soy Proteinate)의 첨가가 육계의 생산성에 미치는 영향

  • Lee, Mun-Ku (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Chan-Ho (Department of Animal Science and Technology, Chung-Ang University) ;
  • Shin, Dong-Hun (Department of Animal Science and Technology, Chung-Ang University) ;
  • Jung, Byoung-Yun (Department of Poultry Science, The University of Georgia) ;
  • Paik, In-Kee (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2011.04.18
  • Accepted : 2011.06.03
  • Published : 2011.06.30


This study was conducted to investigate the effects of dietary supplementation of Cu-sulfate, Cu-methionine chelate (Cu-Met) and Cu-soy proteinate (Cu-SP) on the performance, blood parameters and mineral contents of muscle. It was conducted with a total of 1,000 one d old broilers chickens (Ross$^{(R)}$) which were assigned to four dietary treatments; Control, Cu sulfate (200 ppm Cu as $CuSO_4{\cdot}5H_2O$), Cu-Met (200 ppm Cu as Cu-methionine chelate), Cu-SP (200 ppm Cu as Cu-soy proteinate). There were significant differences (p<0.05) among treatments in weight gain. Weight gain of Cu treated groups were higher than the control during 3~5 wk. There were significant differences (p<0.05) among treatments in feed intake during 0~3 wk. Cu-Met was significantly (p<0.05) lower than the control but the differences among Cu treatments were not significant. There were significant differences (p<0.05) among treatments in feed conversion rate (FCR). Cu treated groups were lower than the control during the whole period. Production efficiency factor (PEF) was significantly higher (p<0.01) in Cu treated groups than the control. Nutrient availabilities of diets were not significantly different among the treatments. The count of white blood cell (WBC) and eosinophil (EO) were lower in Cu-SP treatment than in the control. Copper concentration in the liver was significantly (p<0.01) higher in Cu treated groups than the control. Zinc concentration in the breast and wing muscle was lower in Cu treated and that of leg muscle was higher in Cu-Met than the control. The result of this experiment showed that Cu supplementation at the level of 200 ppm as Cu sulfate, Cu-Met and Cu-SP improves weight gain (4~5 wk), FCR and PEF. Differences among Cu sources were not significant.


  1. AOAC 1990 Official Method of Analysis. 15th Ed. Association of Official Chemist. Washington DC, USA.
  2. Baker DH, Odle J, Funk MA, Wieland TM 1991 Bioavailability of copper in cupric oxide, cuprous, and in a copper-lysine complex. Poult Sci 70(1):171-179.
  3. Burnell TW, Cromwell GL, Stahly TS 1988 Cited by J. Gohl in Bottom Line of Nutrition. Feedstuff. June 13. pp 16-18.
  4. Chiou PWS, Chen KL, Yu B 1997 Toxicity, tissue accumulation and residue in egg and excreta of copper in laying hens. Anim. Feed Sci Technol 67(1):49-60.
  5. Cromwell GL, Stahly TS, Mongue HJ 1989 Effect of source and level of copper on performance and liver copper stores in weaning pigs. J Anim Sci 67(11):2996-3002.
  6. Fisher C 1973 Use of copper sulfate as a growth promotor for broilers. Feedstuffs July 16:24-25.
  7. Hatfield PG, Swenson CK, Kott RW, Ansotegui RP, Roth NJ, Robinson BL 2001 Znic and copper status in ewes supplemented with sulfate- and amino acid- complexed forms of zinc and copper. J Anim Sci 79(1):261-266.
  8. Hawbaker JA, Speer VC, Hays VW, Hawbaker JH, Catron DV 1961 Effects of copper sulfate and other chemotherapeutics in growing swine rations. J Anim Sci 20(1):163-167.
  9. Kim G-B, Seo YM, Shin KS, Rhee AR, Han J, Paik IK 2011 Effects of supplemental copper-methionine chelate and coppersoy proteinate on the performance, blood parameters, liver mineral content, and intestinal microflora of broiler chickens. J Appl Poult Res 20:21-32.
  10. Klasing CK 1998 Minerals. pp 234-276 in Comparative Avian Nutrition. CAB International. New York, USA.
  11. Kornegay ET, van Heugten PHG, Lindermann MD, Blodgett DJ 1989 Effects of biotin and high copper levels on performance and immune response of weanling pigs. J Anim Sci 67(6):1471-1477.
  12. Maurice DV, Jones JE, Bherer NJ 1981 Interraction of Roxarsone and copper in broiler chicks. Bri Poult Sci 28:529
  13. NRC 1994 Nutrient Requirements of Poultry. National Academy Press, Washington DC, USA.
  14. Paik IK 2001a Application of chelated minerals in animal production. Asian-Aust J Anim Sci 14:Special Issue:191-198.
  15. Paik IK 2001b Management of excretion of phosphorus, nitrogen and pharmacological level minerals to reduce environmental pollution from animal production. Asian-Aust J Anim Sci 14(3):384-394.
  16. Paik IK, Seo SH, Um JS, Chang MB, Lee BH 1999 Effects of supplementary copper-chelate on the performance and cholesterol level in plasma and breast muscle of broiler chickens. Asian-Aust J Anim Sci 12(5):794-798.
  17. Roof MD, Mahan DC 1982 Effect of carbadox and various dietary copper level for weaning swine. J Anim Sci 55:1109.
  18. $ROSS^{\circledR}$ Broiler Manual Aviagen 2009 pp. 101.
  19. SAS Institute 1996 $SAS/STAT^{\circledR}$ User's Guide Release. 6.12 Edition. SAS Institute Inc. Cary NC, USA.
  20. Shurson GC, Ku PK, Wacier GL, Yokoyama MT, Miller ER 1990 Physiological relationships between microbiological status and dietary copper levels in the pig. J Anim Sci 68(4): 1061-1071.
  21. Stanly TS, Cromwell GL, Monegue HJ 1980 Effects of the dietary inclusion of copper and antibiotics in the performance of weaning pigs. J Anim Sci 51(6):1347-1351.
  22. Steel RGD, Torrie JH 1980 Principles and Procedures of Statistics. 2nd Ed: McGraw-Hill Publishing Co., NY.
  23. Zhou W, Kornegay ET, Lindemann MD, Swinkels JWGM, Welton MK, Wong EA 1994 Stimulation of growth by intravenous injection of copper in weanling pigs. J Anim Sci 72(9):2395-2403.
  24. 김윤기 백인기 1993 구리 공급원과 첨가 수준이 육계의 생 산성에 미치는 영향. 한국축산학회지 35(1):52-59.
  25. 김윤기 최규형 백인기 1993 황산동의 종류와 지방의 상호작 용이 육계의 생산성에 미치는 영향. 한국영양사료학회지 17(1):7-14.
  26. 민승기 남궁환 백인기 1993 사료첨가제로서 구리복합제가 육계의 생산성에 미치는 영향. 한국영양사료학회지 17(5): 247-257.
  27. 민승기 엄재상 백인기 1994 메치오닌-구리 및 단백질-구리 복합제가 육계와 쥐의 성장과 광물질 대사 및 장내 균총 에 미치는 영향. 한국영양사료학회지 18(2):103-113.
  28. 백인기남궁환최영진1991 황산동과지방의상호작용이육계 의 생산성에 미치는 영향. 한국영양사료학회지 15(6):281- 286.
  29. 이한규 2005 Fe-soy proteinate를 이용한 철분강화계육 및 계 란 생산에 관한 연구. 제103회 중앙대학교 석사학위논문.
  30. 최영진 백인기 1989 유산동의 첨가가 육계의 생산성에 미치 는 영향. 한국영양학회지 13(4):193-200.
  31. 홍성진 임희석 백인기 2002 사료내 Cu 및 Zn-Methionine Chelates 첨가가 육계의 생산성에 미치는 영향. 한국동물자원 과학회지 44(4):399-406.

Cited by

  1. Effects of Supplementary Cu-Soy Proteinate (Cu-SP) and Herbal Mixture (HBM) on the Growth Performance, Intestinal Microflroa, Immune Response in Broilers vol.41, pp.3, 2014,
  2. Growth Performance and Carcass Yield of Heavy Female Broiler at Different Stocking Densities vol.40, pp.1, 2013,
  3. Performance and Carcass Ratio of Large-type Female Broiler at Different Stocking Densities vol.39, pp.4, 2012,