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Uncertainty evaluation of the analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in hair by GC-NCI-MS/MS

GC-NCI-MS/MS를 이용한 모발 중 대마 대사체 분석의 측정불확도 평가

  • Kim, Jin-Young (Drug Analysis Laboratory, Supreme Prosecutors' Office) ;
  • Lee, Jae-Il (Drug Analysis Laboratory, Supreme Prosecutors' Office) ;
  • Cheong, Jae-Chul (Drug Analysis Laboratory, Supreme Prosecutors' Office) ;
  • Suh, Yong-Jun (Drug Analysis Laboratory, Supreme Prosecutors' Office) ;
  • In, Moon-Kyo (Drug Analysis Laboratory, Supreme Prosecutors' Office)
  • Received : 2010.08.17
  • Accepted : 2011.01.25
  • Published : 2011.02.25

Abstract

We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THC-COOH), the metabolite of ${\Delta}^9$-tetrahydrocannabinol, in hair samples by using the bead-assisted liquid-liquid extraction and gas chromatography-tandem mass spectrometric (GC-NCI-MS/MS) detection. Traceability of measurement was established through the use of reference materials, calibrated volumetric tubes, volume measuring devices, and measuring instruments. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day variation was performed by using statistical analysis of several indicative factors. Measurement uncertainty associated with the analyte in real forensic hair samples were estimated using QC data. The major factor of contribution to combined standard uncertainty was inter-day repeatability, while those associated with preparation of analytical standard and also sample of weight were insignificant considering the degree of contribution. Relative uncertainty of relative extended standard uncertainty divided into the measured concentration of the analyte was 17% in a hair sample. The uncertainty of result evaluation will be invaluable to improve quality of the analysis.

Acknowledgement

Supported by : 한국연구재단(KRF)

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