The analysis of ethylene glycol and metabolites in biological specimens

생체시료에서 에틸렌 글리콜과 그 대사체 분석에 관한 연구

  • Park, Seh-Youn (Department of Chemical Analysis, National Forensic Service) ;
  • Kim, Yu-Na (Department of Chemical Analysis, National Forensic Service) ;
  • Kim, Nam-Yee (Department of Chemical Analysis, National Forensic Service)
  • 박세연 (국립과학수사연구원 법과학부 화학분석과) ;
  • 김유나 (국립과학수사연구원 법과학부 화학분석과) ;
  • 김남이 (국립과학수사연구원 법과학부 화학분석과)
  • Received : 2010.09.14
  • Accepted : 2011.03.16
  • Published : 2011.04.25


Ethylene glycol (EG) is produced commercially in large amounts and is widely used as antifreeze or deicing solution for cars, boats, and aircraft. EG poisoning occurs in suicide attempts and infrequently, either intentionally through misuse or accidental as EG has a sweet taste. EG has in itself a low toxicity, but is in vivo broken down to higher toxic organic acids which are responsible for extensive cellular damage in various tissues caused principally by the metabolites glycolic acid and oxalic acid. The most conclusive analytical method of diagnosing EG poisoning is determination of EG concentration. However, victims are sometimes admitted at a late stage to hospitals or died during emergency treatment like a gastric lavage or found rotten dead, when blood EG concentrations are low or not detected. Therefore, in this study, the identification of EG was not only performed by gas chromatograpyc-mass spectrometry (GC-MS) following derivatization but also further toxicological analyses of metabolites, glycolic acid (GA) and oxalic acid (OA), were performed by ion chromatography in various biological specimens. A ranges of blood concentrations (3 cases) was $10\sim2,400\;{\mu}g/mL$ for EG, $224\sim1,164\;{\mu}g/mL$ for GA and ND $\sim40\;{\mu}g/mL$ for OA, respectively, In other biological specimens (liver, kidney, bile and pleural fluid), a range of concentrations (3 cases) was ND $\sim55,000\;{\mu}g/mL$ for EG, ND $\sim1,124\;{\mu}g/mL$ for GA and ND $\sim60\;{\mu}g/mL$ for OA, respectively. Liver and kidney tissues were recommended specimens including blood because OA, a final metabolite of EG, was identified large amounts in these despite no detectable EG caused by some therapy.


  1. P. D. Daniel, J. B. Kenneth, S. H. Robert and S. R. Williams, J. Emergency Medicine, 15(5), 653-667(1997).
  2. P. Hantson, R. Yanbinst and P. Mahieu, Am. J. Forensic. Med. Pathol., 23(2), 159-161(2002).
  3. C. LeBlanc and N. Murphy, Canadian Family Physician, 55, 46-49(2009).
  4. C. LeBlanc and N. Murphy, Canadian Family Physician, 55, 46-49(2009).
  5. H. Wollersen, F. Erdmann, M. Risse and R. Dettmeyer, Legal Medicine, 11, S488-S490 (2009).
  6. C. G. Thomas and Jr., Christina E., Air. Medical. J, 18(3), 114-118(1999).
  7. P. D. Deon, J. Z. Peter and H. H. E. Mary, Pharmacotherapy. 22(3), 365-372(2002).
  8. N. M. Wu and I. M. Theodore, J. Anal. Toxicol., 11, 63-66(1987).
  9. M. Lovric , P. Granic, M. Cubrilo-Turek, Z. Lalic´ and J. Sertic., J. Forensic Sci. Intern., 170, 213-215(2007).
  10. H. H. Yao and W. H. Porter, Clin. Chem., 42, 292-297(1996).
  11. W. H. Porter, P. W. Ruter and H. H. Yao, J. Anal. Toxicol., 23, 591-597(1999).
  12. N. C. Jain and R. Forney, Jr., "Ethylene glycol in methodology for analytical toxicology", 165-166, I. Sunshine, Ed., CRC Press, Inc., West Palm Beach, FL, U.S.A., 1975.
  13. R. O. Bost and I. Sunshine, J. Anal. Toxicol. 4, 102-103(1980).
  14. H. W. Porter and A. Auansakul, Clin. Chem. 28(1), 75-78(1982).
  15. A. Dasgupta, W. Blackwell, J. Grie and S. Malik., J. Chromatography B, 666, 63-70(1995).
  16. A. F. Eder, C. M. McGath and Y. G. Dowdy, Clin. Chem. 44, 168-177(1998).
  17. E. W. McChesney, L. Goldberg and C. K. Parehk, et al., Food Cosmet Toxicol. 9, 21-38(1971).
  18. A. F Eder, C. M. McGrath and Y. G. Dowdy, Clin. Chem. 44, 168-177(1998).
  19. H. M. Qing, K. Adeli, G. A. Zello and H. P. William, J. Krahn, Clin. Chem. Acta, 411, 601-604(2010).
  20. P. M. Leth and M. Gregersen, J. Forensic Science International, 155, 179-184(2005)
  21. C. B. Randall and H. C. Robert, "Disposition of Toxic Drugs and Chemicals in Man", 3rd ed., 336-341, Year book medical publishers, Inc., U.S.A., 1989.
  22. C. Alvin, M. D. Bronstein, A. Daniel, P. H. D. Spyker, R. Louis, P. H. D. Cantilena, JR, L. Jody, P. H. D. Green, H. Barry, M. D. Rumack, L. Sandra and Giffin, Clinical Toxicology, 47, 911-1084(2009).

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