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Analytical method of aflatoxins in edible oil and infant-children foods

식용유지와 영유아식품 중 아플라톡신 분석방법

  • Hu, Soo-Jung (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Park, Seung-Young (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Kim, Soon-Sun (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Lee, Joon-Goo (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Song, Ji-Young (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Kang, Eun-Gi (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Lee, Hyun-Sook (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Cho, Dae-Hyun (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration)
  • 허수정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 박승영 (경인지방식품의약품안전청 유해물질분석과) ;
  • 김순선 (경인지방식품의약품안전청 유해물질분석과) ;
  • 이준구 (경인지방식품의약품안전청 유해물질분석과) ;
  • 송지영 (경인지방식품의약품안전청 유해물질분석과) ;
  • 강은귀 (경인지방식품의약품안전청 유해물질분석과) ;
  • 이현숙 (경인지방식품의약품안전청 유해물질분석과) ;
  • 조대현 (경인지방식품의약품안전청 유해물질분석과)
  • Received : 2011.02.25
  • Accepted : 2011.03.21
  • Published : 2011.04.25

Abstract

Aflatoxins are secondary metabolites of the molds of Aspergillus flavus and Aspergillus parasiticus. They are highly carcinogenic compounds and can affect a wide range of vegetable commodities such as cereals (especially corn), nuts, peanuts, fruits and oil seeds, in the field and during storage. In fact, oilseeds are often stored for weeks in conditions that promote the mould growth, and the possible consequent presence of aflatoxins in oilseeds can lead to their transfer in oil. In addition, aflatoxins can be found as a natural contaminant in multi-cereals and beans making baby food for infants and young-children. The objective of this study was to validate the liquid extraction method or develop an analytical method for edible oil and infant-children foods. Therefore, this study developed condition of extract for aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) in edible oil using a high performance liquid chromatography with florescence detector (HPLC/FLD). Aflatoxins were extracted from edible oil samples by means of MSPD (Matrix solid phased dispersion), utilizing $C_{18}$ as dispersing material and purified by using immunoaffinity column. The gression line coefficients were above 0.999. The recoveries for aflatoxins ranged from 85.9 to 93.0%, and relative standard deviations were below 5.7%. The new developed method of aflatoxins effectively enhanced recoveries by using MSPD-Immunoaffinity column compared with liquid extraction. The analytical method for liquid extraction of aflatoxin was appropriate for infant-children food. Reviewing the current method, the recoveries of aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) were 89.5~92.3%.

Keywords

edible oil;infant-children food;aflatoxins;analysis

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