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Determination of superdrol and its metabolites in human urine by LC/TOF-MS and GC/TOF-MS

LC/TOF-MS와 GC/TOF-MS를 이용한 인체 내 요시료 중 Superdrol과 그 대사체의 분석

  • Choi, Hae-Min (Clean Energy Center, Korea Institute of Science and Technology) ;
  • Yum, Tae-Woo (Clean Energy Center, Korea Institute of Science and Technology) ;
  • Paeng, Gi-Jeong (Department of chemistry, Yonsei University) ;
  • Kim, Yun-Je (Clean Energy Center, Korea Institute of Science and Technology)
  • 최해민 (한국과학기술연구원, 청정에너지센터) ;
  • 염태우 (한국과학기술연구원, 청정에너지센터) ;
  • 팽기정 (연세대학교, 화학과) ;
  • 김연제 (한국과학기술연구원, 청정에너지센터)
  • Received : 2011.04.28
  • Accepted : 2011.05.31
  • Published : 2011.06.25

Abstract

This study was done for the determination and excretion profile of superdrol and its metabolites in human urine using both liquid chromatography with electrospray ionization mass spectrometry and gas chromatography with mass spectrometry after trimethylsilylation. Superdrol and its two metabolites were detected in human urine after administration of superdrol to healthy volunteers. The intra-day recovery ranged 89.7-113.2%, accuracy ranged 91.8-113.8% and reproducibility ranged 0.2-6.8% and inter-day recovery ranged 89.3-104.1%, accuracy ranged 95.2-103.0%, reproducibility ranged 0.7-7.8%. We found that superdrol M1 was a hydration at C-3 and superdrol M2 was a hydroxylation at D-ring. Superdrol and two metabolites were excreted as their glucuronided fractions. The glucuro-/sulfa-conjugated ratio of superdrol, superdrol M1 and superdrol M2 were 0.02, 0.02, 0.01, respectively. The excretion studies showed that superdrol and two metabolites were reached 4.3 h after oral administration and superdrol and superdrol M1 were detected until 48 h in human urine.

Keywords

superdrol;metabolite;LC/MS;GC/MS

Acknowledgement

Supported by : KIST

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