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The Roles of Phytohormones and AtEXPA3 Gene in Gravitropic Response of Arabidopsis thaliana

애기장대 굴중성 반응에 있어서 식물호르몬과 AtEXPA3 유전자의 역할

  • Yun, Hye-Sup (Department of Life Science, Chung-Ang University) ;
  • Lee, Yew (Department of Biological Science and Technology, Yonsei University) ;
  • Kim, Seong-Ki (Department of Life Science, Chung-Ang University)
  • 윤혜섭 (중앙대학교 생명과학과) ;
  • 이유 (연세대학교 생명과학기술학부) ;
  • 김성기 (중앙대학교 생명과학과)
  • Received : 2011.02.07
  • Accepted : 2011.06.24
  • Published : 2011.07.30

Abstract

We focused on relationship between phytohormones and AtEXPA3 gene in gravitropic response of A. thaliana. RT-PCR analysis shows that AtEXPA3 was highly expressed in actively developing tissues such as leaf, rosette, root and flower tissues. AtEXPA3 gene expression was enhanced by gravistimulation, BR and IAA. Furthermore, decreased gravitropism was observed when treatment of AVG, an ethylene biosynthetic inhibitor, suggesting that ethylene has a gravistimulating effect itself as well as BRs and IAA. Inhibition of gravitropism in AtEXPA3 RNAi mutant suggests that BR, auxin and ethylene are playing roles as regulators of AtEXPA3. In addition, altered gravitropism in BRs signaling mutant (decreased in bri1-301, bak1, and increased BRI-GFP) indicated that BRs signaling mediated the gravitropism. In conclusion, gravitropic responses of Arabidopsis root resulting from root growth were mediated by increased expression of AtEXPA3 gene, which is stimulated by phytohormones.

Acknowledgement

Supported by : 중앙대학교

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