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Immuno-enhancing Effects of Lactobacillus salivarius JWS 58 and Lactobacillus plantarum JWS 1354 isolated from duck

오리로부터 분리한 Lactobacillus salivarius JWS 58과 Lactobacillus plantarum JWS 1354 균주의 면역활성효과

  • Choi, Hyun Jong (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Ji Ye (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Shin, Myeong Su (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Sang Myeong (Division of Biotechnology, College of Environmental & Bioresource Sciences, Chonbuk National University) ;
  • Lee, Wan Kyu (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 최현종 (충북대학교 수의과대학) ;
  • 김지예 (충북대학교 수의과대학) ;
  • 신명수 (충북대학교 수의과대학) ;
  • 이상명 (전북대학교 환경생명자원대학 생명공학부) ;
  • 이완규 (충북대학교 수의과대학)
  • Received : 2011.10.10
  • Accepted : 2011.12.02
  • Published : 2011.12.30

Abstract

Lactobacillus salivarius JWS 58 (JWS 58) and Lactobacillus plantarum JWS 1354 (JWS 1354) are isolated from duck intestine and have ability to produce bacteriocin. The objective of this study was to evaluate the immunomodulatory effects of JWS 58 and JWS 1354. The nitric oxide (NO) and cytokines (IL-$1{\beta}$ and TNF-${\alpha}$) were measured in C57BL/6 mouse peritoneal macrophages to determine immune enhancing effects of JWS 58 and JWS 1354. A Listeria (L.) monocytogenes challenge mice model was used to evaluate immune enhancement ability of JWS 58 and JWS 1354 in vivo. The results showed that JWS 58 and JWS 1354 increased the production of NO or cytokines by peritoneal macrophages and that oral administration of viable probiotic strains in mice elicited the immuno-modulatory effect upon L. monocytogenes challenge. JWS 1354 showed stronger immune enhancing effects than JWS 58. Collectively, this study demonstrated that Lactobacillus strain JWS 58 and JWS 1354 possess immune enhancing effect. Furthermore, two stains are expected to use feed supplement to prevent diseases by pathogenic bacteria through releasing bacteriocin and enhancing host immune responses in animal.

Acknowledgement

Supported by : 중소기업청

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