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Protective effect of ethyl acetate extract of Ishige okamurae against carbon tetrachloride-induced acute liver injury in rats

  • Kang, Sohi (Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Yang, Wonjun (Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Oh, Hanseul (Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Bae, Yeonji (Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Ahn, Meejung (Department of Anatomy, College of Medicine, Jeju National University) ;
  • Kang, Min Chul (Jeju Technopark) ;
  • Ko, Ryeo Kyeong (Jeju Technopark) ;
  • Kim, Gi Ok (Jeju Technopark) ;
  • Lee, Jun Hwa (Department of Pediatrics, Samsung Changwon Hospital, School of Medicine, Sungkyunkwan University) ;
  • Hyun, Jin Won (Department of Biochemistry, College of Medicine, Jeju National University) ;
  • Moon, Changjong (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Taekyun (Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Received : 2011.08.17
  • Accepted : 2011.09.27
  • Published : 2011.12.30

Abstract

Several compounds and extracts isolated from a brown alga, Ishige (I.) okamurae, exhibit anti-oxidant and anti-inflammatory effects. The present study investigated whether the ethyl acetate (EtOAc) fraction of I. okamurae (EFIO) could ameliorate carbon tetrachloride ($CCl_{4}$)-induced hepatotoxicity in rats. Sprague-Dawley rats were intraperitoneally (i.p.) administered with EFIO at 10 or 50 mg/kg per day for 2 consecutive days before $CCl_{4}$ injection (3.3 mL/kg, i.p.). Twenty four hours later, the rats were anesthesized with diethyl ether and dissected. Pretreatment with EFIO significantly reduced the increased serum levels of alanine aminotransferase and aspartate aminotransferase in $CCl_{4}$-treated rats. Pretreatment with EFIO also significantly inhibited the reduced activities of superoxide dismutase and catalase in the $CCl_{4}$-injured liver. Histopathological evaluations showed that hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration induced by $CCl_{4}$ treatment were ameliorated by the administration of EFIO. Additionally, liver immunohistochemical analyses revealed the marked reduction in ED1-positive monocyte-like macrophages in EFIO-pretreated rats given $CCl_{4}$. These results suggest that EFIO ameliorates $CCl_{4}$-induced liver injury, possibly through the inhibition of oxidative stress.

References

  1. Ahn M, Moon C, Yang W, Ko EJ, Hyun JW, Joo HG, Jee Y, Lee NH, Park JW, Ko RK, Kim GO, Shin T. Diphlorethohydroxycarmalol, isolated from the brown algae Ishige okamurae, protects against radiation-induced cell damage in mice. Food Chem Toxicol 2011, 49, 864-870. https://doi.org/10.1016/j.fct.2010.12.008
  2. Carrillo MC, Kanai S, Nokubo M, Kitani K. (-) Deprenyl induces activities of both superoxide dismutase and catalase but not of glutathione peroxidase in the striatum of young male rats. Life Sci 1991, 48, 517-521. https://doi.org/10.1016/0024-3205(91)90466-O
  3. Edwards MJ, Keller BJ, Kauffman FC, Thurman RG. The involvement of Kupffer cells in carbon tetrachloride toxicity. Toxicol Appl Pharmacol 1993, 119, 275-279. https://doi.org/10.1006/taap.1993.1069
  4. elSisi AED, Earnest DL, Sipes IG. Vitamin A potentiation of carbon tetrachloride hepatotoxicity: role of liver macrophages and active oxygen species. Toxicol Appl Pharmacol 1993, 119, 295-301. https://doi.org/10.1006/taap.1993.1072
  5. Halliwell B, Gutteridge JMC. The importance of free radicals and catalytic metal ions in human diseases. Mol Aspects Med 1985, 8, 89-193. https://doi.org/10.1016/0098-2997(85)90001-9
  6. Hayashi S, Itoh A, Isoda K, Kondoh M, Kawase M, Yagi K. Fucoidan partly prevents $CCl_{4}$-induced liver fibrosis. Eur J Pharmacol 2008, 580, 380-384. https://doi.org/10.1016/j.ejphar.2007.11.015
  7. Heo SJ, Hwang JY, Choi JI, Han JS, Kim HJ, Jeon YJ. Diphlorethohydroxycarmalol isolated from Ishige okamurae, a brown algae, a potent $\alpha$-glucosidase and alpha-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. Eur J Pharmacol 2009, 615, 252-256. https://doi.org/10.1016/j.ejphar.2009.05.017
  8. Heo SJ, Kim JP, Jung WK, Lee NH, Kang HS, Jun EM, Park SH, Kang SM, Lee YJ, Park PJ, Jeon YJ. Identification of chemical structure and free radical scavenging activity of diphlorethohydroxycarmalol isolated from a brown alga, Ishige okamurae. J Microbiol Biotechnol 2008, 18, 676-681.
  9. Hsu YW, Tsai CF, Chuang WC, Chen WK, Ho YC, Lu FJ. Protective effects of silica hydride against carbon tetrachloride-induced hepatotoxicity in mice. Food Chem Toxicol 2010, 48, 1644-1653. https://doi.org/10.1016/j.fct.2010.03.039
  10. Kim KN, Heo SJ, Yoon WJ, Kang SM, Ahn G, Yi TH, Jeon YJ. Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-$\kappa$B and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages. Eur J Pharmacol 2010, 649, 369-375. https://doi.org/10.1016/j.ejphar.2010.09.032
  11. Kim MM, Rajapakse N, Kim SK. Anti-inflammatory effect of Ishige okamurae ethanolic extract via inhibition of NF-$\kappa$B transcription factor in RAW 264.7 cells. Phytother Res 2009, 23, 628-634. https://doi.org/10.1002/ptr.2674
  12. Lee IK, Kang JW. A check list of marine algae in Korea. Korean J Phycol 1986, 1, 311-325.
  13. McCay PB, Lai EK, Poyer JL, DuBose CM, Janzen EG. Oxygen- and carbon-centered free radical formation during carbon tetrachloride metabolism. Observation of lipid radicals in vivo and in vitro. J Biol Chem 1984, 259, 2135-2143.
  14. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972, 247, 3170-3175.
  15. Perez Tamayo R. Is cirrhosis of the liver experimentally produced by $CCl_{4}$ and adequate model of human cirrhosis? Hepatology 1983, 3, 112-120.
  16. Zou Y, Qian ZJ, Li Y, Kim MM, Lee SH, Kim SK. Antioxidant effects of phlorotannins isolated from Ishige okamurae in free radical mediated oxidative systems. J Agric Food Chem 2008, 56, 7001-7009. https://doi.org/10.1021/jf801133h