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Molecular Characterization of Fusarium Graminearum Virus 2 Isolated from Fusarium graminearum Strain 98-8-60

  • Yu, Ji-Suk (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University) ;
  • Lee, Kyung-Mi (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University) ;
  • Son, Moon-Il (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University)
  • Received : 2011.03.11
  • Accepted : 2011.06.10
  • Published : 2011.09.01

Abstract

Fusarium graminearum virus 2 (FgV2) infects Fusarium graminearum strain 98-8-60 and has at least five segments of double-stranded RNAs (dsRNAs), denoted as dsRNA-1 to dsRNA-5. In this study, the genome of FgV2 was sequenced and its phylogenetic relationship with other mycoviruses was analyzed. The lengths of FgV2 dsRNAs 1-5 ranged from 2414 to 3580 base pairs (bp). The 5' and 3' untranslated regions (UTRs) are highly conserved, and each dsRNA segment had 78-105 and 84-306 bp of 5' and 3' UTRs, respectively. Each dsRNA segment contained a single open reading frame (ORF). Computer analysis of dsRNA-1 revealed a putative open reading frame (ORF) that shows high sequence identity with an RNA-dependent RNA polymerase (RdRp) containing eight conserved motifs. dsRNAs 2-5 also each contain one putative ORF coding for products of unknown function. The sequences of FgV2 dsRNA-2 and dsRNA-3 have significant sequence identity with Magnaporthe oryzae chrysovirus 1 (MoCV1) dsRNA-3 and -4, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis of the putative RdRp protein, FgV2 was found to form a distinct virus clade with Aspergillus mycovirus 1816 and MoCV1 in the family Chrysoviridae.

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