Effects of Carbohydrate and Water Temperature on Nutrient and Energy Digestibility of Juvenile and Grower Rockfish, Sebastes schlegeli

  • Lee, Sang-Min (Department of Marine Bioscience and Technology, Gangneung-Wonju National University) ;
  • Pham, Minh-Anh (Department of Marine Bioscience and Technology, Gangneung-Wonju National University)
  • Received : 2011.07.05
  • Accepted : 2011.09.20
  • Published : 2011.11.01


A factorial ($4{\times}2{\times}2$) experimental design was employed to determine apparent digestibilities of dry matter (DM), protein, lipid, energy and nitrogen-free extract (NFE) of the test diets containing either ${\alpha}$-potato starch (A-PS), ${\beta}$-potato starch (B-PS), ${\beta}$-corn starch (B-CS) or dextrin (DEX) as dietary carbohydrate energy for juvenile (average weight 30 g) and grower (average weight 300 g) rockfish reared at 13$^{\circ}C$ and 20$^{\circ}C$. Chromic oxide was used as an inert marker. Feces were collected by fecal collectors attached to rearing tanks from triplicate groups of juvenile and grower rockfish. Digestibilities of DM, energy and NFE of the test diets were significantly affected by dietary carbohydrate and water temperature (p<0.01), but not by fish size. DM digestibility of the fish fed the A-PS diet was significantly higher than that of fish fed other treatments, except for the DEX diet at 20$^{\circ}C$. DM digestibility of rockfish fed the B-CS diet was significantly lower than that of other diets. A similar pattern was observed in apparent digestibility of energy. NFE digestibility of fish fed the test diets was significantly affected by carbohydrate and significantly correlated to DM (r = 0.97, p<0.01) and energy (r = 0.99, p<0.01) digestibilities, regardless of water temperature and fish size. NFE digestibility of the fish fed the ${\beta}$-starch diets was relatively lower compared to that of the ${\alpha}$-starch diets, and ranged from 35 to 43% and 20 to 27% for B-PS and B-CS, respectively. The present findings indicate that carbohydrate and water temperature significantly affected digestibilities of dry matter, energy and nitrogen-free extract of rockfish. Among dietary carbohydrates, ${\alpha}$-potato starch could be effectively used as dietary carbohydrate energy for rockfish at 13$^{\circ}C$ and 20$^{\circ}C$.


Rockfish;Sebastes schlegeli;Carbohydrate;Water Temperature;Digestibility


  1. Amirkolaie, A. K., J. A. J. Verreth and J. W. Schrama. 2006. Effect of gelatinization degree and inclusion level of dietary starch on the characteristics of digesta and faeces in Nile tilapia (Oreochromis niloticus (L.)). Aquaculture 260:194-205.
  2. Bergot, F. 1993. Digestibility of native starch of various botanical origins by rainbow trout (Oncorhynchus mykiss). In: Fish Nutrition in Practice, Proc. of the IV Int. Symp. on Nutrition and Feeding (Ed. S. J. Kaushik and P. Luquet). Les Colloques INRA, 61:857-865.
  3. Catacutan, M. R. and R. M. Coloso. 1998. Growth of juvenile Asian sea bass, Lates calcarifer, fed varying carbohydrate and lipid levels. Aquaculture 149:137-144.
  4. Cho, C. Y. and S. J. Slinger. 1979. Apparent digestibility measurement in feedstuffs for rainbow trout. In: Finfish Nutrition and Fishfeed Technology (Ed. J. E. Halver and K. Tiews). Heeneman, Berlin, 239-248.
  5. Cowey, C. B. 1988. The nutrition of fish: the developing scene. Nutr. Res. Rev. 1:255-280.
  6. Duncan, D. B. 1955. Multiple-range and multiple F test. Biometrics 11:1-42.
  7. Fange, A. and D. Grove. 1979. Digestion. In: Fish Physiology (Ed. W. S. Hoar, D. J. Randall and J. R. Bretts). Academic Press. New York. pp. 161-260.
  8. Furukuwa, A. and H. Tsukahara. 1966. On the acid digestion for the determination of chromic oxide as an index substance in the study of digestibility of fish feed. Bull. Jpn. Soc. Sci. Fish. 32:502-506.
  9. Gaylord, T. G. and D. M. Gatlin III. 1996. Determination of digestibility coefficients of various feedstuffs for red drum (Sciaenops ocellatus). Aquaculture 139:303-314.
  10. Grisdale-Helland, B. and S. J. Helland. 1997. Replacement of protein by fat and carbohydrate in diets for Atlantic salmon (Salmo salar) at the end of the freshwater stage. Aquaculture 152:167-180.
  11. Grisdale-Helland, B. and S. J. Helland. 1998. Macronutrient utilization by Atlantic halibut (Hippoglossus hippoglossus): diet digestibility and growth of 1 kg fish. Aquaculture 166:57-65.
  12. Guillaume, J., S. Kaushik, P. Bergot and R. Metailler. 2001. Carbohydrate nutrition: importance and limits of carbohydrate supplies. In: Nutrition and Feeding of Fish and Crustaceans (Ed. J. Guillaume, S. Kaushik, P. Bergot and R. Metailler). Springer, Chichester, UK. pp. 131-143.
  13. Hilton, J. W., J. I. Atkinson and S. J. Slinger. 1983. Effects of increased dietary fiber on the growth of rainbow trout (Salmo gairdneri). Can. J. Fish. Aqua. Sci. 40:81-85.
  14. Hutchins, C. G., S. D. Rawles and D. M. Gatlin III. 1998. Effects of dietary carbohydrate kind and level on growth, body composition and glycemic response of juvenile sunshine bass (Morone chrysops ${\times}$M. saxatilis ${\times}$). Aquaculture 161:187-199.
  15. Jobling, M. 2001. Feed composition and analysis. In: Food Intake in Fish (Ed. D. Houlihan, T. Boujard and M. Jobling). Blackwell Science. France.
  16. Kaushik, S. J. and F. Medale. 1994. Energy requirements, utilization and dietary supply to salmonids. Aquaculture 124: 81-97.
  17. Kirchgessner, M., H. Kurzinger and F. J. Schwarz. 1986. Digestibility of crude nutrients in the different feeds and estimation of their energy contents for carp (Cyprinus carpio L.). Aquaculture 58:185-194.
  18. Lee, S.-M. 2002. Apparent digestibility coefficients of various feed ingredients for juvenile and grower rockfish (Sebastes schlegeli). Aquaculture 207:79-95.
  19. Lee, S.-M., K.-D. Kim and S. P. Lall. 2003. Utilization of glucose, maltose, dextrin and cellulose by juvenile flounder (Paralichthys olivaceus). Aquaculture 221:427-438.
  20. Lovell, T. 1989. Digestion and metabolism. In: Nutrition and Feeding of Fish (Ed. T. Lovell). Van Nostrand Reinhold, New York.
  21. Lupatsch, I., G. W. M. Kissil, D. Sklan and E. Pfeffer. 1997. Apparent digestibility coefficients of feed ingredients and their predictability in compound diets for gilthead seabream, Sparus aurata L. Aqua. Nutr. 3:81-89.
  22. McGoogan, B. B. and R. C. Reigh. 1996. Apparent digestibility of selected ingredients in red drum (Sciaenops ocellantus) diets. Aquaculture 141:233-244.
  23. Medale, F., P. Aguirre and S. J. Kaushik. 1991. Utilization of dietary carbohydrates by rainbow trout at two water temperatures. In: Energy Metabolism of Farm Animals (Ed. C. Wenk and M. Boessinger). EAAP Publication, 58, Pudoc, Wageningen, pp. 392-395.
  24. Morris, P. C. 1997. Nutritional needs of bass and bream. Fish Farmer International File. November/December, 28-31.
  25. Munilla-Moran, R. and F. Saborido-Rey. 1996. Digestive enzymes in marine fish. II. Amylase activities in gut from seabream (Sparus aurata), turbot (Scophthalmus maximus) and redfish (Sebastes mentella). Comp. Biochem. Physiol. 113B:827-834.
  26. National Research Council. 1993. Nutritional requirement of fish. National Academic Press. Washington, DC, p. 114.
  27. Peragon, J., J. B. Barroso, L. Garcia-Salguero, M. de la Higuera and J. A. Lupianez. 1999. Carbohydrates affect proteinturnover rates, growth, and nucleic acid content in the white muscle of rainbow trout (Oncorhynchus mykiss). Aquaculture 179:425-437.
  28. Peres, H. and A. Oliva-Teles. 2002. Utilization of raw and gelatinized starch by European sea bass (Dicentrarchus labrax) juveniles. Aquaculture 205:287-299.
  29. Podoskina, T. A., A. G. Podoskin and E. N. Bekina. 1997. Efficiency of utilization of some potato starch modifications by rainbow trout (Oncorhynchus mykiss). Aquaculture 152:235-248.
  30. Rawles, S. D. and D. M. Gatlin III. 1998. Carbohydrate utilization in striped bass (Morone saxatilis) and sunshine bass (M. chrysops×M. saxatilis). Aquaculture 161:201-212.
  31. Shiau, S. Y. 1997. Utilization of carbohydrates in warmwater fishwith particular reference to tilapia, Oreochromis niloticus${\times}$O. aureus. Aquaculture 151:79-96.
  32. Stone, D. A. J., G. L. Allan and A. J. Anderson. 2003. Carbohydrate utilization by juvenile silver perch, Bidyanus bidyanus (Mitchell). II. Digestibility and utilization of starch and its breakdown products. Aqua. Res. 34:109-121.
  33. Storebakken, T. 2002. Atlantic Salmon, Salmon salar. In: Nutrient Requirement and Feeding of Finfish for Aquaculture (Ed. C. D. Webster and C. Lim). CAB International, 2000.
  34. Storebakken, T., K. D. Shearer, S. Reftie, S. Lagocki and J. McCool. 1998. Interactions between sanility, dietary carbohydrate source and carbohydrate concentration on the digestibility of macronutrients and energy in rainbow trout (Oncorhynchus mykiss). Aquaculture 163:347-359.
  35. Takeuchi, T. 1991. Digestion and nutrition. In: Fish Physiology (Ed. Y. M. Itazawa and I. Hanyu). Kouseisha-Kouseikaku, Tokyo, pp. 67-101.
  36. Takeuchi, T., S. Satoh and V. Kiron. 2002. Common carp, Cyprynus carpio. In: Nutrient Requirement and Feeding of Finfish for Aquaculture (Ed. C. D. Webster and C. Lim). CAB International.
  37. Vergara, J. M. and K. Jauncey. 1993. Studies on the use of dietary energy by gilthead seabream (Sparus aurata L.) juveniles. In: Fish Nutrition in Practices (Ed. S. J. Kaushik and P. Luquet). Proceeding of the 4th International Symposium on Fish Nutrition and Feeding. INRA Editions, Paris, France.

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