- Volume 24 Issue 11
DOI QR Code
Early Growth Response of Broilers to Dietary Lysine at Fixed Ratio to Crude Protein and Essential Amino Acids
Panda, A.K.;Rao, S.V. Rama;Raju, M.V.L.N.;Lavanya, G.;Reddy, E. Pradeep Kumar;Sunder, G. Shyam
- Received : 2011.03.26
- Accepted : 2011.06.22
- Published : 2011.11.01
The effects of dietary lysine (Lys) at a fixed ratio to crude protein (CP) and essential amino acids (EAA) on early growth response of broilers were studied. Four diets were formulated to contain similar metabolizable energy (ME, 2,950 kcal/kg) but contained graded levels of incremental Lys (1.1, 1.2, 1.3 and 1.4%) while also increasing the dietary CP and EAA (methionine, methionine+ cystine, threonine and tryptophan) to maintain a constant ratio with Lys. Each diet was fed at random to 10 replicates of 6 chicks each throughout the experimental period (1-21 d). At the lowest concentration of Lys of 1.1% (19.04% CP), body weight gain (BWG) was lowest and feed conversion ratio (FCR) was poorest. The BWG increased and FCR decreased linearly as dietary Lys increased upto 1.3% (22.5% CP). Lowest feed consumption was observed in the dietary group that contained 1.1% Lys (19.04% CP) in the diet. Increasing the concentration of Lys to 1.2% (20.77% CP), significantly increased the feed consumption. The concentrations of protein, calcium, phosphorus and cholesterol in serum were not influenced by the variation in Lys contents in the diet. The humoral immune response as measured by antibody titre in response to SRBC inoculation was significantly lower in the diets containing 1.1% Lys compared to 1.4%. It is concluded that the Lys requirement of broilers is 1.3% (22.5% CP) during 0 to 21 days of age for eliciting optimum performance when a fixed ratio of Lys to CP (1:17.31) and essential AA is maintained (1:0.47 Met; 1:0.56 Thr; 1:0.17 Try).
Lysine;Crude Protein;Essential Amino Acid;Early Growth Response;Broilers
- Abebe, S. and T. R. Morris. 1990. Note on the effect of protein concentration of responses to dietary lysine by chicks. Br. Poult. Sci. 31:255-260. https://doi.org/10.1080/00071669008417255
- Aftab, U., M. Ashraf and Z. Jiang. 2006. Low protein diets for broilers. World's Poult. Sci. J. 62:688.698. https://doi.org/10.1079/WPS2005121
- Aletor, V. A., I. I. Hamid, E. Nieb and E. Pfeffer. 2000. Lowprotein amino acid -supplemented diets in broiler chickens: Effects on performance, carcass characteristics, whole body composition and efficiencies of nutrient utilization. J. Sci. Food Agric. 80:547-554. https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<547::AID-JSFA531>3.0.CO;2-C
- Almquist, H. J. 1952. Amino acid requirements of chickens and turkey - a review. Poult. Sci. 31:969-981.
- AOAC. 1990. Official methods of analysis. 15th ed. Association of Official Analytical Chemists. Arlington, Virginia, USA.
- AOAC. 2006. Official methods of analysis. 18th ed. Association of Official Analytical Chemists Int., Washington, DC.
- Baker, D. H., A. B. Batal, T. M. Parr, N. R. Augspurger and C. M. Parsons. 2002. Ideal ratio (relative to lysine) of tryptophan, threonine, isoleucine and valine for chicks during the second and third weeks post hatch. Poult. Sci. 81:485-494. https://doi.org/10.1093/ps/81.4.485
- Bilgili, S. F., E. T. Moran Jr., and N. Acar. 1992. Strain cross response of heavy male broilers to dietary lysine in the finisher feed: Live performance and further processing yields. Polt. Sci. 71:850-858. https://doi.org/10.3382/ps.0710850
- Cook, M. E. 1991. Nutrition and the immune response of the domestic fowl. Cri. Rev. Poult. Biol. 3:167-189.
- Doumas, B. T., W. A. Watson and H. G. Biggs. 1971. Albumin standard and the measurement of serum albumin with bromocresol green. Clin. Chem. Acta 31:87. https://doi.org/10.1016/0009-8981(71)90365-2
- Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics 11:1-42. https://doi.org/10.2307/3001478
- Fiske, H. and Y. Subba Row. 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66:375-400.
- Grau, C. R. 1948. Effect of protein level of the lysine requirement of the chick. J. Nutr. 36:99-108.
- Gous, R. M. and T. R. Morris. 1985. Evaluation of a diet dilution technique for measuring the response of broiler chickens to increasing concentrations of lysine, Br. Poult. Sci. 26:147-161. https://doi.org/10.1080/00071668508416799
- Jiang, Q., P. W. waldroup and C. A. Fritts. 2005. Improving utilization of diets low in crude protein fro beoiler chicks, 1. Evaluation of specific amino acid supplementation to diets low in crude protein. Int. J. Poult. Sci. 4:115-122. https://doi.org/10.3923/ijps.2005.115.122
- Katanbaf, M. N., E. A. Dunnington and P. B. Siegel. 1988. Allomorphic relationships from hatching to 56 days in parental lines and F1 crosses of chickens selected 27 generations for high or low body weight. Growth Dev. Aging 52:11-22.
- Kidd, M. T. 2004. Nutritional modulation of immune function in broilers. Poult. Sci. 83:650-657. https://doi.org/10.1093/ps/83.4.650
- Kidd, M. T., A. Corzo, D. Hoehler, E. R. Miller and W. A. Dozier III. 2005. Broiler responsiveness (Ross X 708) to diet varying in amino acid density. Poult. Sci. 84:1389-1396. https://doi.org/10.1093/ps/84.9.1389
- Kidd, M. T. and B. I. Fancher. 2001. Lysine needs of starting chicks and subsequent effects during the growth period. J. Appl. Poult. Res. 10:385-393. https://doi.org/10.1093/japr/10.4.385
- Klasing, K. C. and D. M. Barnes. 1988. Decreased amino acid requirements of growing chick due to immunological stress. J. Nutr. 118:1158-1164.
- Latshaw, J. D. 1991. Nutrition-mechanisms of immunosuppression. Vet. Immunol. Immunopathol. 30:111-112. https://doi.org/10.1016/0165-2427(91)90012-2
- Lemme, A. 2003. The "ideal" protein concept in broiler nutrion 2. Experimental data on varying dietary ideal protein levels. Pages 7-14 in Amino News volume 4(2). Degussa AG, Hanau, Germany.
- Maatman, R., W. B. Gross, E. A. Dunnington, A. S. Larsen and P. B. Siegel. 1993. Growth, immune response and behavior of broiler and Leghorn cockerels fed different methionine levels. Arch. Geflugel. 57:249-256.
- Moran, E. T. Jr., R. D. Bushong and S. F. Bilgili. 1992. Reducing dietary crude protein of broilers while satisfying amino acid requirements by least cost formulation: live performance, litter composition and yield. Poult. Sci. 71:1687-1694. https://doi.org/10.3382/ps.0711687
- Morris, T. R., K. Alazzawi, R. M. Gous and G. L. Simson. 1987. Effect of protein concentration of responses to dietary lysine by chicks. Br. Poult. Sci. 28:185-195. https://doi.org/10.1080/00071668708416952
- Morris, T. R., R. M. Gous and C. Fisher. 1999. An analyses of the hypothesis that amino acid requirements for chicks should be stated as a proportion of dietary protein. World's Poult. Sci. J. 55:7-22. https://doi.org/10.1079/WPS19990002
- National Research Council. 1984. Nutrient requirement of poultry. 8th rev. ed. National Academic Press, Washington, DC.
- National Research Council. 1994. Nutrient requirements of poultry. 9th rev. ed. National Academic Press, Washington, DC.
- Panda, A. K., M. V. L. N. Raju, S. V. Rama Rao, G. Lavanya, E. Pradeep Kumar Reddy and G. Shyam Sunder. 2010. Replacement of normal maize with quality protein maize on performance, immune response and carcass characteristics of broiler chickens. Asian-Aust. J. Anim. Sci. 23:1626-1631. https://doi.org/10.5713/ajas.2010.10036
- Pinchasov, Y., C. X. Mendonca and L. S. Jensen. 1990. Broiler chick response to low protein diets supplemented with synthetic amino acid. Poult. Sci. 69:1950-1955. https://doi.org/10.3382/ps.0691950
- Plumstead, P. W., N. D. Romero-Sanchez, N. D. Paton, J. W. Spears and J. Brake. 2007. Effects of dietary metabolizable energy and protein on early growth responses of broilers to dietary lysine. Poult. Sci. 86:2639-2648. https://doi.org/10.3382/ps.2007-00168
- Praharaj, N. K., M. R. Reddy, A. K. Panda, S. V. Rama Rao and R. P. Sharma. 2002. Genotype by dietary interaction for growth and response to sheep red blood cells and Escherchia coli inoculation in commercial broiler chicks. Asian-Aust. J. Anim. Sci. 15:1170-1177. https://doi.org/10.5713/ajas.2002.1170
- Quentin, M., I. Bouvarel and M. Picard. 2005. Effects of the starter diet, light intensity, and essential amino acids level of growth and carcass composition of broilers. J. Appl. Poult. Res. 14:64-76.
- Si, J., C. A. Fritts, D. J. Burnham and P. W. Waldroup. 2004. Extent to which crude protein may be reduced in corn-soybean meal broiler diets through amino acid supplementation. Int. J. Poult. Sci. 31:46-50.
- Siegel, P. B., W. B. Gross and J. A. Cherry. 1982. Correlated response of chicken to selection for production of antibody to sheep erythrocytes. Animal Blood Groups. Biochem. Gen. 13:291-297.
- Snedecor, G. W. and W. G. Cochran. 1989. Statistical methods. Oxford and IBH Publishing Company, New Delhi.
- Surisdiarto, J, and D. J. Farrell. 1991. The relationship between dietary CP and dietary lysine requirement by broiler chicks on diets with and with out the "ideal" amino acid balance. Poult. Sci. 70:830-836. https://doi.org/10.3382/ps.0700830
- Velu, J. G., D. H. Baker and H. M. Scott. 1971. Protein and energy utilization by chicks fed graded levels of a balanced mixture of crystalline amino acids. J. Nutr. 101:1249-1256.
- Waguspeak, A. M., S. Powell, T. D. Bidner, R. L. Payne and L. L. Southern. 2009. Effect of incremental levels of L-lysine and determination of the limiting amino acids in low crude protein corn-soybean meal diets for broilers. Poult. Sci. 88:1216-1226. https://doi.org/10.3382/ps.2008-00452
- Wegmann, T. G. and O. Smithies. 1966. A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion 6: 67-73. https://doi.org/10.1111/j.1537-2995.1966.tb04696.x
- Zlatkis, A., B. Zak and A. J. Boyle. 1953. A new method for the direct determination of serum cholesterol. J. Lab. Clin. Med. 41:486-492.
- Effect of nutrient density on production performance, egg quality and humoral immune response of brown laying (Dahlem Red) hens in the tropics vol.44, pp.2, 2012, https://doi.org/10.1007/s11250-011-0017-9
- Validation of the Lysine Requirement as Reference Amino Acid for Ideal In-Feed Amino Acid Ratios in Modern Fast Growing Meat-Type Chickens vol.06, pp.03, 2016, https://doi.org/10.4236/ojas.2016.63024
- Effects of Nutrient Density and exogenous enzymesin Starter Diet on Performance, Intestinal Microflora, Gut Morphology and Immune Response of Broiler Chickens vol.19, pp.3, 2017, https://doi.org/10.1590/1806-9061-2017-0501
- Effects of dietary lysine supplementation on performance, egg quality, and development of reproductive system in egg-laying ducks pp.0974-1844, 2017, https://doi.org/10.1080/09712119.2017.1308868
- Effect of Dietary Lysine Regimens on Growth Performance and Meat Composition in Aseel Chicken vol.20, pp.2, 2018, https://doi.org/10.1590/1806-9061-2017-0584